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Title
Japanese: 
English:Critical Amino Acid Residues for the Specific Binding of the Ti-RecognizingRecombinant Ferritin with Oxide Surfaces of Titanium and Silicon 
Author
Japanese: 林 智広, 佐野健一, 芝清隆, 岩堀健治, 山下 一郎, 原 正彦.  
English: Tomohiro Hayashi, Ken-Ichi Sano, Kiyotaka Shiba, Kenji Iwahori, Ichiro Yamashita, Masahiko Hara.  
Language English 
Journal/Book name
Japanese: 
English:Langmuir 
Volume, Number, Page Vol. 25    No. 18    p. 10901-10906
Published date July 2009 
Publisher
Japanese: 
English:American Chemical Society 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1021/la901242q
Abstract The interactions of ferritins fused with a Ti-recognizing peptide (RKLPDA) and their mutants with titanium oxide substrates were explored with an atomic force microscope (AFM). The amino acid sequence of the peptide was systematically modified to elucidate the role of each amino acid residue in the specific interaction. Force measurements revealed a clear correlation among the sequences in the N-terminal domain of ferritin, surface potentials, and long-range electrostatic interactions. Measurements of adhesion forces clearly revealed that hydrogen bonds take part in the specific binding as well as the electrostatic interaction between charged residues and surface charges of Ti oxides. Moreover, our results indicated that not only the charged and polar residues but also a neutral residue (proline) govern the strength of the specific binding, with the order of the residues also being significant. These results demonstrate that the local structure of the peptide governs the special arrangement of charged residues and strongly affects the strength of the bindings.

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