Separation stress of adhered surfaces between an elastic body and a rigid body is expressed as a function of the elastic modulus when surface roughness and defects exist on the surface of the rigid body. The change of the separation stress against the elastic modulus is important because the elastic modulus may vary with the temperature or other effects after the bodies are adhered together. Therefore, the condition of the increase and decrease of the separation stress is clarified considering the relation among the elastic modulus, the amplitude of the surface roughness and the defect size.