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Title
Japanese: 
English:Mechanical stability of a microscope setup working at a few kelvins for single-molecule localization 
Author
Japanese: 日野原拓也, 濱田裕紀, 中村一平, 松下道雄, 藤芳 暁.  
English: Takuya Hinohara, yuuki hamada, Ippei Nakamura, Michio Matsushita, SATORU FUJIYOSHI.  
Language English 
Journal/Book name
Japanese: 
English:Chemical Physics 
Volume, Number, Page Vol. 419        p. 246-249
Published date June 20, 2013 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1016/j.chemphys.2013.02.024
Abstract A great advantage of single-molecule fluorescence imaging is the localization precision of molecule beyond the diffraction limit. Although longer signal-acquisition yields higher precision, acquisition time at room temperature is normally limited by photobleaching, thermal diffusion, and so on. At low temperature of a few kelvins, much longer acquisition is possible and will improve precision if the sample and the objective are held stably enough. The present work examined holding stability of the sample and objective at 1.5 K in superfluid helium in the helium bath. The stability was evaluated by localization precision of a point scattering source of a polymer bead. Scattered light was collected by the objective, and imaged by a home-built rigid imaging unit. The standard deviation of the centroid position determined for 800 images taken continuously in 17 min was 0.5 nm in the horizontal and 0.9 nm in the vertical directions.

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