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Title
Japanese:Thioredoxin pathway in anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C. 
English:Thioredoxin pathway in anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C. 
Author
Japanese: 若林憲一.  
English: Ken-Ichi Wakabayashi.  
Language English 
Journal/Book name
Japanese:Journal of biochemistry 
English:Journal of biochemistry 
Volume, Number, Page        
Published date Feb. 2021 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1093/jb/mvab014
 
DOI https://doi.org/10.1093/jb/mvab014
Abstract To understand the physiological role of NADPH-thioredoxin reductase C (NTRC) in cyanobacteria, we investigated an NTRC-deficient mutant strain of Anabaena sp., PCC 7120, cultivated under different regimes of nitrogen supplementation and light exposure. The deletion of ntrC did not induce a change in the cell structure and metabolic pathways. However, time-dependent changes in the abundance of specific proteins and metabolites were observed. A decrease in chlorophyll a was correlated with a decrease in chlorophyll a biosynthesis enzymes and PSI subunits. The deletion of ntrC led to a deregulation of nitrogen metabolism, including the NtcA accumulation and heterocyst-specific proteins while nitrate ions were available in the culture medium. Interestingly, this deletion resulted in a redox imbalance, indicated by higher peroxide levels, higher catalase activity, and the induction of chaperones such as MsrA. Surprisingly, the antioxidant protein 2-Cys Prx was down-regulated. The deficiency in ntrC also resulted in the accumulation of metabolites such as 6-phosphogluconate, ADP, and ATP. Higher levels of NADP+ and NADPH partly correlated with higher G6PDH activity. Rather than impacting protein expression levels, NTRC appears to be involved in the direct regulation of enzymes, especially during the dark to light transition period.

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