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Title
Japanese:Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation. 
English:Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation. 
Author
Japanese: Yuhei Chadani, Sugata N, 丹羽達也, Ito Y, Shintaro Iwasaki, Hideki Taguchi.  
English: Yuhei Chadani, Sugata N, Tatsuya Niwa, Ito Y, Shintaro Iwasaki, Hideki Taguchi.  
Language English 
Journal/Book name
Japanese:The EMBO journal 
English:The EMBO journal 
Volume, Number, Page        
Published date Oct. 20, 2021 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://europepmc.org/articles/PMC8634131
 
DOI https://doi.org/10.15252/embj.2021108299
Abstract Continuous translation elongation, irrespective of amino acid sequences, is a prerequisite for living organisms to produce their proteomes. However, nascent polypeptide products bear an inherent risk of elongation abortion. For example, negatively charged sequences with occasional intermittent prolines, termed intrinsic ribosome destabilization (IRD) sequences, weaken the translating ribosomal complex, causing certain nascent chain sequences to prematurely terminate translation. Here, we show that most potential IRD sequences in the middle of open reading frames remain cryptic and do not interrupt translation, due to two features of the nascent polypeptide. Firstly, the nascent polypeptide itself spans the exit tunnel, and secondly, its bulky amino acid residues occupy the tunnel entrance region, thereby serving as a bridge and protecting the large and small ribosomal subunits from dissociation. Thus, nascent polypeptide products have an inbuilt ability to ensure elongation continuity.

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