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Title
Japanese:Thioredoxin pathway in Anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C. 
English:Thioredoxin pathway in Anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C. 
Author
Japanese: Deschoenmaeker F, Mihara S, 丹羽達也, Taguchi H, Ken-ichi Wakabayashi, Toyoshima M, Shimizu H, Hisabori T.  
English: Deschoenmaeker F, Mihara S, Tatsuya Niwa, Taguchi H, Ken-ichi Wakabayashi, Toyoshima M, Shimizu H, Hisabori T.  
Language English 
Journal/Book name
Japanese:Journal of biochemistry 
English:Journal of biochemistry 
Volume, Number, Page        
Published date Sept. 1, 2021 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1093/jb/mvab014
 
DOI https://doi.org/10.1093/jb/mvab014
Abstract To understand the physiological role of NADPH-thioredoxin reductase C (NTRC) in cyanobacteria, we investigated an NTRC-deficient mutant strain of Anabaena sp., PCC 7120, cultivated under different regimes of nitrogen supplementation and light exposure. The deletion of ntrC did not induce a change in the cell structure and metabolic pathways. However, time-dependent changes in the abundance of specific proteins and metabolites were observed. A decrease in chlorophyll a was correlated with a decrease in chlorophyll a biosynthesis enzymes and photosystem I subunits. The deletion of ntrC led to a deregulation of nitrogen metabolism, including the NtcA accumulation and heterocyst-specific proteins while nitrate ions were available in the culture medium. Interestingly, this deletion resulted in a redox imbalance, indicated by higher peroxide levels, higher catalase activity and the induction of chaperones such as MsrA. Surprisingly, the antioxidant protein 2-CysPrx was downregulated. The deficiency in ntrC also resulted in the accumulation of metabolites such as 6-phosphogluconate, ADP and ATP. Higher levels of NADP+ and NADPH partly correlated with higher G6PDH activity. Rather than impacting protein expression levels, NTRC appears to be involved in the direct regulation of enzymes, especially during the dark-to-light transition period.

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