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タイトル
和文: 
英文:Novel pyrene-based two-photon active fluorescent dye efficiently excited and emitting in the ‘tissue optical window (650–1100 nm)’ 
著者
和文: 小西玄一, 仁子陽輔.  
英文: Gen-ichi Konishi, Yosuke Niko.  
言語 English 
掲載誌/書名
和文: 
英文:Journal of Materials Chemistry B 
巻, 号, ページ Vol. 3    No. 2    pp. 184–190
出版年月 2015年1月15日 
出版者
和文: 
英文:Royal Society of Chemistry (RSC) 
会議名称
和文: 
英文: 
開催地
和文: 
英文: 
ファイル
公式リンク http://pubs.rsc.org/en/content/articlelanding/2014/tb/c4tb01404a#!divAbstract
 
DOI http://dx.doi.org/10.1039/C4TB01404A
アブストラクト The development of two-photon (TP) active fluorophores remains an important issue. Dyes that can be excited and fluoresce efficiently in the ‘tissue optical window’ (650–1100 nm) are especially in demand to maximize the underlying performance of two-photon fluorescence microscopy (TPFM) as an advanced optical technique. Ideally, such dyes would be compatible with the 1050 nm femtosecond fibre laser, which has recently been developed as an inexpensive excitation source to make the TPFM technique universal. In this work, we designed and synthesized a novel pyrene-based acceptor–π–acceptor (A–π–A) dye, PY, which exhibited outstanding properties such as bright fluorescence (λem = 650 nm and ΦFL = 0.80) and a large two-photon absorption cross-section (1100 GM (1 GM = 10−50 cm4 per photon per molecule) at 950 nm and 380 GM at 1050 nm) in the tissue optical window. In living mitochondria, PY provided more sensitive microscopic images than current dyes and showed great potential to be a building block of TP active fluorescent probes for the 1050 nm fibre laser. We believe that the exceptional properties of PY will be extended to other fluorescent probes through further chemical modification.

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