Reactive species have been widely studied to cause various disorders and diseases by reacting inside and outside of the body, and some of them have harmful effects on the human body. We can generate various reactive species in atmospheric low temperature plasma using discharges and by using atmospheric low temperature plasma, we can irradiate reactive species to living organisms and cells, which can be applied to the study of the effects of reactive species on living organisms.
We have developed an atmospheric pressure low-temperature plasma jet devices that can generate various gas plasmas. The plasma device is small enough to be held in the palm of the hand and injects plasma of about 30 to 60℃ generated by electrode-to-electrode discharge at a gas flow rate of several L/min. It has been shown that the bactericidal effect against E. coli depends on the types of gas.
In this study, in order to verify whether the inactivation effect can also be obtained for viruses, virus inactivation experiments were conducted using atmospheric pressure low-temperature plasma, and the differences in the inactivation effect depending on the gas type and treatment time were evaluated. Virus inactivation experiments were performed by bubbling a plasma of several gases into 50 mL of virus suspension prepared to an infection titer of about 104-5 TCID50/mL. The virus solution was collected at each treatment time, and after stepwise dilution, each was dropped into cells and incubated in a 5% CO2 incubator at 37℃ for 4 to 6 days, and the TCID50 was calculated from the cell denaturation effect.
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