Optogenetic induction of hibernation-like state with modified human Opsin4 in mice
著者
和文:
Tohru M., Takahashi,
Arisa, Hirano,
Takeshi, Kanda,
Viviane M., Saito,
Hiroto, Ashitomi,
Kazumasa Z., Tanaka,
横式康史,
Kosaku, Masuda,
Masashi, Yanagisawa,
Kaspar E., Vogt,
Takashi, Tokuda,
Takeshi, Sakurai.
英文:
Tohru M., Takahashi,
Arisa, Hirano,
Takeshi, Kanda,
Viviane M., Saito,
Hiroto, Ashitomi,
Kazumasa Z., Tanaka,
Yasufumi Yokoshiki,
Kosaku, Masuda,
Masashi, Yanagisawa,
Kaspar E., Vogt,
Takashi, Tokuda,
Takeshi, Sakurai.
言語
English
掲載誌/書名
和文:
英文:
Cell Reports Methods
巻, 号, ページ
Vol. 2
No. 11
pp. 100336
出版年月
2022年10月
出版者
和文:
英文:
Cell Press
会議名称
和文:
英文:
開催地
和文:
英文:
アブストラクト
We recently determined that the excitatory manipulation of Qrfp-expressing neurons in the preoptic area of the hypothalamus (quiescence-inducing neurons [Q neurons]) induced a hibernation-like hypothermic/hypometabolic state (QIH) in mice. To control the QIH with a higher time resolution, we develop an optogenetic method using modified human opsin4 (OPN4; also known as melanopsin), a G protein-coupled-receptor-type blue-light photoreceptor. C-terminally truncated OPN4 (OPN4dC) stably and reproducibly induces QIH for at least 24 h by illumination with low-power light (3 μW, 473 nm laser) with high temporal resolution. The high sensitivity of OPN4dC allows us to transcranially stimulate Q neurons with blue-light-emitting diodes and non-invasively induce the QIH. OPN4dC-mediated QIH recapitulates the kinetics of the physiological changes observed in natural hibernation, revealing that Q neurons concurrently contribute to thermoregulation and cardiovascular function. This optogenetic method may facilitate identification of the neural mechanisms underlying long-term dormancy states such as sleep, daily torpor, and hibernation.
各種検索
サポート
ヘルプ