@book{CTT100775335, author = {Yuki Omuro}, title = {A novel protein-protein interaction assay based on the functional complementation of mutant firefly luciferases: Split structure versus divided reaction.}, publisher = {}, year = 2018, } @book{CTT100775340, author = {Yuki Omuro}, title = {Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases.}, publisher = {}, year = 2017, } @article{CTT100902331, author = {Yuki Ohmuro-Matsuyama and Hayato Matsui and Masaki Kanai and Tadaomi Furuta}, title = {Glow-type conversion and characterization of a minimal luciferase via mutational analyses}, journal = {FEBS J.}, year = 2023, } @article{CTT100887436, author = {Yuki Ohmuro-Matsuyama and Hayato Matsui and Masaki Kanai and Tadaomi Furuta}, title = {Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.0}, journal = {Biotechnol. J.}, year = 2023, } @article{CTT100885450, author = {Sung Bae Kim and Tadaomi Furuta and Yuki Ohmuro-Matsuyama and Nobuo Kitada and Ryo Nishihara and Shojiro A. Maki}, title = {Bioluminescent imaging systems boosting near-infrared signals in mammalian cells}, journal = {Photochem. Photobiol. Sci.}, year = 2023, } @article{CTT100867224, author = {Yuki Ohmuro-Matsuyama and Tadaomi Furuta and Hayato Matsui and Masaki Kanai and Hiroshi Ueda}, title = {Miniaturization of bright light-emitting luciferase ALuc: picALuc}, journal = {ACS Chem. Biol.}, year = 2022, } @article{CTT100862791, author = {Yuki Ohmuro-Matsuyama and Keiko Gomi and Takuya Shimoda and Hideki Yamaji and Hiroshi Ueda}, title = {Improving the stability of protein–protein interaction assay FlimPIA using a thermostabilized firefly luciferase}, journal = {Frontiers in Bioengineering and Biotechnology}, year = 2021, } @article{CTT100848715, author = {Riho Takahashi and Takanobu Yasuda and Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {BRET Q‐Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase−Dye−Antibody Fusion with Enhanced Response}, journal = {Analytical Chemistry}, year = 2021, } @article{CTT100847093, author = {Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Kimura and Hiroshi Ueda}, title = {Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies}, journal = {ACS Omega}, year = 2021, } @article{CTT100838310, author = {Yoshitaka HARADA and Yuki OHMURO-MATSUYAMA and Mika TSUNA and Hiroshi UEDA}, title = {An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens}, journal = {Analytical Sciences}, year = 2020, } @article{CTT100836504, author = {Akihito Inoue and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q‐body for Rapid Signal-On Detection of Small Hapten Methotrexate}, journal = {ACS Sensors}, year = 2020, } @article{CTT100821930, author = {Jinhua Dong and Yuya Oka and Hee-Jin Jeong and Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex}, journal = {Biotechnology and Bioengineering}, year = 2020, } @article{CTT100814312, author = {Kanai, Y. and Ohmuro-Matsuyama, Y. and Tanioku, M. and Ushiba, S. and Ono, T. and Inoue, K. and Kitaguchi, T. and Kimura, M. and Ueda, H. and Matsumoto, K.}, title = {Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens}, journal = {ACS Sensors}, year = 2020, } @article{CTT100814313, author = {Jiulong Su and Tetsuya Kitaguchi and Yuki Ohmuro-Matsuyama and Theresa Seah and Farid J. Ghadessy and Shawn Hoon and Hiroshi Ueda}, title = {Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens}, journal = {Scientific Reports}, year = 2019, } @article{CTT100836860, author = {Chan-I Chung and Yuko Sato and Yuki Ohmuro-Matsuyama and Shinichi Machida and Hitoshi Kurumizaka and Hiroshi Kimura and Hiroshi Ueda}, title = {Intrabody-based FRET probe to visualize endogenous histone acetylation}, journal = {Scientific Reports}, year = 2019, } @article{CTT100799987, author = {Jiulong Su and Cyrus Beh and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Shawn Hoon and Hiroshi Ueda}, title = {Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens}, journal = {Journal of Bioscience and Bioengineering}, year = 2019, } @article{CTT100829107, author = {Renee Dale and Yuki Ohmuro-Matsuyama and Hiroshi Ueda and Naohiro Kato}, title = {Non-steady state analysis of enzyme kinetics in real time elucidates substrate association and dissociation rates: demonstration with analysis of firefly luciferase mutants}, journal = {BIOCHEMISTRY-US}, year = 2019, } @article{CTT100814311, author = {Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {Protein-Protein Interaction Assays Using Split-NanoLuc}, journal = {Bioluminescence - Analytical Applications and Basic Biology}, year = 2019, } @article{CTT100775336, author = {Yuki Omuro}, title = {Effects of lithium on the secretory production of recombinant antibody from insect cells.}, journal = {In Vitro Cellular & Developmental Biology – Animal}, year = 2018, } @article{CTT100772438, author = {Yuki Ohmuro-Matsuyama and Takahiro Yamashita and Keiko Gomi and Hideki Yamaji and Hiroshi Ueda}, title = {Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage}, journal = {Analytcal Biochemistry}, year = 2018, } @article{CTT100775337, author = {Yuki Omuro}, title = {Modifications of a signal sequence for antibody secretion from insect cells.}, journal = {Cytotechnology}, year = 2018, } @article{CTT100761172, author = {Jiulong Su and Jinhua Dong and Tetsuya Kitaguchi and Yuki Omuro and Hiroshi Ueda}, title = {Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation}, journal = {Analyst}, year = 2018, } @article{CTT100759296, author = {Yuki Omuro and H. Ueda}, title = {Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation}, journal = {Analytical Chemistry}, year = 2018, } @article{CTT100753653, author = {Hee-Jin Jeong and Takuya Kawamura and Manami Iida and Yumi Kawahigashi and Mutsumi Takigawa and Yuki Ohmuro-Matsuyama and Chan-I Chung and Jinhua Dong and Masuo Kondoh and Hiroshi Ueda}, title = {Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin}, journal = {Analytical Chemistry}, year = 2017, } @article{CTT100745301, author = {Yuki Omuro and Takahiro Yamashita and Lin Huan and Hideki Yamaji and Hiroshi Ueda}, title = {Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity}, journal = {Luminescence}, year = 2017, } @article{CTT100775339, author = {Yuki Omuro}, title = {Efficient production of antibody Fab fragment by transient gene expression in insect cells.}, journal = {}, year = 2017, } @article{CTT100737665, author = {Y. Ohmuro and H. Ueda}, title = {Ultrasensitive Firefly luminescent intermediate-based Protein-protein Interaction Assay (FlimPIA) based on the functional complementation of mutant firefly luciferases}, journal = {Methods in Molecular Biology}, year = 2017, } @article{CTT100718390, author = {Chan-I Chung and Ryoji Makino and Yuki Ohmuro and Hiroshi Ueda}, title = {Development of a fluorescent protein-antibody FRET probe for the detection and imaging of osteocalcin}, journal = {J. BIOSCI. BIOENG.}, year = 2016, } @article{CTT100737451, author = {Y. Ohmuro and H. Ueda}, title = {A protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases}, journal = {Methods Mol. Biol.}, year = 2016, } @article{CTT100715953, author = {Yuki Omuro and Mori, K. and Hamada, H. and Hiroshi Ueda and Yamaji, H.}, title = {Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production.}, journal = {CYTOTECHNOLOGY}, year = 2016, } @article{CTT100711186, author = {Renee Dale and Yuki Ohmuro-Matsuyama and Hiroshi Ueda and Naohiro Kato}, title = {Mathematical model of the firefly luciferase complementation assay reveals a non-linear relationship between the detected luminescence and the affinity of the protein pair being analyzed}, journal = {PLOS ONE}, year = 2016, } @article{CTT100665621, author = {Hiroshi Ueda and Yuko Hara and Yuki Ohmuro}, title = {Improved protein-protein interaction assay FlimPIA by the entrapment of luciferase conformation}, journal = {Anal. Chem.}, year = 2013, } @inproceedings{CTT100916895, author = {Tadaomi Furuta and Yuki Ohmuro-Matsuyama}, title = {Towards further enhancement of the activity of the minimal luciferase picALuc}, booktitle = {}, year = 2024, } @inproceedings{CTT100916893, author = {Yuki Ohmuro-Matsuyama and Genta Kamiya and Kento Motoyama and Mitsuru Hattori and Ryogo Takai and Nobuo Kitada and Takeharu Nagai and Shojiro Maki and Hayato Matsui and Tadaomi Furuta}, title = {Towards longer luminescence lifetime of the minimal luciferase picALuc}, booktitle = {}, year = 2024, } @inproceedings{CTT100916868, author = {大室有紀 and 松井勇人 and 叶井正樹 and 古田忠臣}, title = {Glow発光する最小サイズ発光酵素picALuc変異体の作製}, booktitle = {}, year = 2023, } @inproceedings{CTT100916867, author = {大室有紀 and 松井勇人 and 叶井正樹 and 古田忠臣}, title = {荷電ペプチドを付加して最小ルシフェラーゼの活性を高める: picALuc2.0}, booktitle = {}, year = 2023, } @inproceedings{CTT100899581, author = {大室有紀 and 北口哲也 and 上田宏 and 木村宏}, title = {YFP断片とintrabodyを利用した生細胞内におけるエピジェネティクス変化の観察法構築}, booktitle = {第16回日本エピジェネティクス研究会年会 要旨集}, year = 2023, } @inproceedings{CTT100884291, author = {大室有紀 and 松井勇人 and 叶井正樹 and 古田忠臣}, title = {世界最小発光酵素「picALuc」の創出とその後}, booktitle = {}, year = 2022, } @inproceedings{CTT100883091, author = {大室(松山)有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {最小サイズの発光酵素「picALuc」の開発とその応用}, booktitle = {}, year = 2022, } @inproceedings{CTT100883090, author = {大室有紀 and 金誠培 and 松井勇人 and 叶井正樹 and 古田忠臣}, title = {最小の発光酵素「picALuc」の開発とその応用}, booktitle = {}, year = 2022, } @inproceedings{CTT100875069, author = {大室有紀 and 松井勇人 and 叶井正樹 and 古田忠臣}, title = {新規小型発光酵素picALucのアプリケーション開発}, booktitle = {}, year = 2022, } @inproceedings{CTT100869546, author = {大室有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {発光酵素の特性を利用したタンパク質間相互作用検出系、及び新規小型発光酵素の開発}, booktitle = {}, year = 2022, } @inproceedings{CTT100864151, author = {大室有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {明るい小型発光酵素 "picALuc"}, booktitle = {}, year = 2021, } @inproceedings{CTT100875144, author = {Akihito Inoue and Yuki Ohmuro and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of mini Q-body using the nanobody to detect chemotherapy agent.}, booktitle = {}, year = 2021, } @inproceedings{CTT100875142, author = {Akihito Inoue and Yuki Ohmuro and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of mini Q-body using the nanobody to detect chemotherapy agent.}, booktitle = {}, year = 2021, } @inproceedings{CTT100854299, author = {Yuki Ohmuro and Tadaomi Furuta and Hayato Matsui and Masaki Kanai and Hiroshi Ueda}, title = {Development of the smallest luciferase “picALuc” among bright-light emitting luciferases}, booktitle = {}, year = 2021, } @inproceedings{CTT100875130, author = {上田宏 and 有坂亮汰 and 朴俊泰 and 董金華 and 柴田貴広 and 大室有紀 and 北口哲也 and 内田浩二}, title = {ゲノム編集法による酸化脂質類の非競合的免疫測定系の構築}, booktitle = {第74回日本酸化ストレス学会・第21回日本NO学会 合同学術集会要旨集}, year = 2021, } @inproceedings{CTT100837725, author = {Cyrus Beh and Theresa Seah and Jiulong Su and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Ueda and Shawn Hoon.}, title = {Facile method for creating arrays of lipid-capped elastomer microwells for bioinspired protocellular sensors.}, booktitle = {}, year = 2019, } @inproceedings{CTT100837723, author = {Jiulong Su and Cyrus Beh and Tetsuya Kitaguchi and Yuki Ohmuro and Theresa Seah and Shawn Hoon and Hiroshi Ueda.}, title = {Creation of a novel sensor enzyme and its integration into protocell for digital detection of various anti-epitope tag antibodies.}, booktitle = {}, year = 2019, } @inproceedings{CTT100837722, author = {上田宏 and 安田貴信 and 上野慶行 and 井上暁人 and 髙橋昌樹 and 大室有紀 and 北口哲也 and 董金華}, title = {Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築}, booktitle = {第 71回日本生物工学会大会要旨集}, year = 2019, } @inproceedings{CTT100837731, author = {大室有紀 and 北口哲也 and 木村宏 and 上田宏}, title = {YFP断片融合抗体プローブを用いたヒストン翻訳後修飾検出系の構築}, booktitle = {第 71回日本生物工学会大会要旨集}, year = 2019, } @inproceedings{CTT100837729, author = {井上暁人 and 大室有紀 and 北口哲也 and 上田宏}, title = {ナノ抗体を用いた抗がん剤センサーmini Q-body の構築}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837726, author = {上野慶行 and 安田貴信 and 大室有紀 and 北口哲也 and 上田宏}, title = {N末にNanoluc由来タグを持つ抗体断片は抗原依存的にNanolucの発光活性を増大させる}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837728, author = {大室有紀 and 北口哲也 and 木村宏 and 上田宏}, title = {分割YFP融合Intrabodyを用いた生細胞内ヒストンアセチル化検出プローブの構築}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100771381, author = {上田宏 and 大室有紀 and 北口哲也}, title = {均相系測定を可能にする抗原応答性標識抗体の創製}, booktitle = {}, year = 2018, } @inproceedings{CTT100771380, author = {高橋 里帆 and 大室 有紀 and 上田 宏}, title = {自ら光るクエンチ抗体BRET Q-bodyの発光特性}, booktitle = {第12回バイオ関連化学シンポジウム講演要旨集}, year = 2018, } @inproceedings{CTT100768650, author = {Hiroshi Ueda and Yuki Ohmuro}, title = {Homogeneous noncompetitive immunological detection of small molecules by ternary luciferase fragment complementation (OS-BLIA)}, booktitle = {}, year = 2018, } @inproceedings{CTT100753069, author = {高橋里帆 and 大室有紀 and 上田 宏}, title = {発光酵素融合クエンチ抗体を用いた生物発光抗原検出系の構築}, booktitle = {第69回日本生物工学会大会講演要旨集}, year = 2017, } @inproceedings{CTT100745300, author = {高橋里帆 and 大室有紀 and 上田 宏}, title = {発光酵素融合クエンチ抗体を用いたBRET抗原検出系の構築}, booktitle = {第17回日本蛋白質科学会年会要旨集}, year = 2017, } @inproceedings{CTT100737020, author = {三宅 千絢 and 鄭 煕陳 and 大室 有紀 and 董 金華 and 上田 宏}, title = {天然抗体をQuenchbody化するための抗体結合蛍光プローブの開発}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100737019, author = {田中 絵美理 and 鍾 蝉伊 and 大室 有紀 and 董 金華 and 上田 宏}, title = {生物発光共鳴エネルギー移動を利用した蛋白質認識抗体プローブの開発}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100737022, author = {大室 有紀 and 上田 宏 and 山地 秀樹}, title = {リチウム添加による昆虫細胞からの抗体分泌生産の向上}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100735729, author = {田中絵美理 and 鐘蝉伊 and 大室有紀 and 上田宏}, title = {血清アルブミン検出のための生物発光抗体プローブの開発}, booktitle = {第89回日本生化学会大会要旨集}, year = 2016, } @inproceedings{CTT100724180, author = {大室有紀}, title = {小胞体シグナル配列の改変が昆虫細胞による抗体分泌生産に及ぼす影響}, booktitle = {}, year = 2016, } @inproceedings{CTT100724179, author = {大室有紀}, title = {ヒカリコメツキムシ由来発光酵素Emerald lucを用いた蛋白質間相互作用検出系FlimPIAの構築}, booktitle = {}, year = 2016, } @inproceedings{CTT100724643, author = {大室有紀 and 五味恵子 and 山地秀樹 and 上田宏}, title = {蛋白質間相互作用検出系FlimPIAの実用化を目指したプローブの熱安定性向上}, booktitle = {BMB2015要旨集}, year = 2015, } @inproceedings{CTT100724183, author = {大室有紀 and 五味恵子 and 山地秀樹 and 上田宏}, title = {蛋白質間相互作用検出系FlimPIAにおけるプローブの熱安定性向上}, booktitle = {}, year = 2015, } @inproceedings{CTT100724182, author = {上田宏 and 五味恵子 and 山地秀樹 and 大室有紀}, title = {ヘイケボタル酵素を用いた蛋白質間相互作用検出系FlimPIAの構築}, booktitle = {}, year = 2015, } @inproceedings{CTT100724184, author = {大室 有紀 and 森 慶太 and 濱田 宏嗣 and 山地 秀樹}, title = {小胞体シグナル配列の改変が昆虫細胞による抗体分泌生産に及ぼす影響}, booktitle = {}, year = 2015, } @inproceedings{CTT100674610, author = {上田宏 and 大室有紀}, title = {Functional complementation of luciferase half-reactions: application to a robust protein-protein interaction assay FlimPIA}, booktitle = {Luminescence}, year = 2014, } @misc{CTT100925686, author = {古田忠臣 and 大室(松山)有紀}, title = {最小サイズの発光酵素picALucの開発とその性能向上}, year = 2024, } @misc{CTT100915146, author = {大室(松山)有紀 and 古田忠臣}, title = {最小サイズの発光酵素picALucの開発と改良}, year = 2024, } @misc{CTT100769945, author = {Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction}, year = 2018, } @misc{CTT100775338, author = {Yuki Omuro}, title = {たんぱく質 高感度検出}, year = 2018, } @misc{CTT100724191, author = {大室有紀}, title = {生物発光・蛍光消光を利用した新規計測技術の開発}, year = 2015, } @misc{CTT100669481, author = {大室有紀 and 上田 宏}, title = {ホタル発光酵素の反応機構を利用したタンパク質間相互作用検出系の開発}, year = 2014, } @misc{CTT100869590, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/021899(2020/06/03), WO 2020/246495(2020/10/12)} } @misc{CTT100917020, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {登録特許}, year = 2024, month = {}, note = {特願2021-524873(2020/06/03), WO 2020/246495(2020/10/12), 特許第7461661号(2024/03/27)} } @misc{CTT100869602, author = {上田宏 and 北口哲也 and 大室有紀 and 上野慶行}, title = {レポータータンパク質断片を付加した組換え抗体を用いるホモジニアス免疫測定法}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/020281(2020/05/22), WO 2020/241486(2020/12/03)} } @misc{CTT100869583, author = {上田宏 and SuJiulong and 北口哲也 and 大室有紀}, title = {ホモジニアス免疫測定法に適した酵素変異体}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/002264(2020/01/23), WO 2020/162203(2020/08/13)} } @misc{CTT100930807, author = {上田宏 and SuJiulong and 北口哲也 and 大室有紀}, title = {ホモジニアス免疫測定法に適した酵素変異体}, howpublished = {登録特許}, year = 2024, month = {}, note = {特願2020-571094(2020/01/23), WO 2020/162203(2020/08/13), 特許第7606210号(2024/12/17)} } @misc{CTT100831058, author = {上田宏 and SuJiulong and 北口哲也 and 大室有紀 and Shawn Hoon and Cyrus Beh }, title = {バイオセンサー}, howpublished = {登録特許}, year = 2022, month = {}, note = {特願2018-207624(2018/11/02), 特開2020-068746(2020/05/07), 特許第7169582号(2022/11/02)} } @misc{CTT100820743, author = {上田宏 and 大室有紀 and 三宅千絢 and 塚原知也}, title = {抗原検出又は測定用キット}, howpublished = {登録特許}, year = 2021, month = {}, note = {特願2018-566814(2018/01/17), 再表2018-147018(2019/12/12), 特許第6983419号(2021/11/26)} } @misc{CTT100675219, author = {上田宏 and 大室 有紀 and 和田 猛}, title = {ホタルが光る仕組みを利用した「たんぱく質の出会い」検出技術を開発}, year = 2010, note = {2010-2013}, }