@book{CTT100830112, author = {KOJIMA Takaaki and ZHU Bo and NAKANO Hideo}, title = {Construction of a DNA Library on Microbeads Using Whole Genome Amplification.}, publisher = {}, year = 2015, } @article{CTT100831162, author = {Bo Zhu and Rui Gan and Maria D. Cabezas and Takaaki Kojima and Robert Nicol and Michael C. Jewett and Hideo Nakano}, title = {Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins}, journal = {Biotechnology and Bioengineering}, year = 2020, } @article{CTT100926938, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Mitsue Nagamine and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody}, journal = {Biosensors and Bioelectronics}, year = 2024, } @article{CTT100908475, author = {Kana Sasamoto and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M}, journal = {The Analyst}, year = 2023, } @article{CTT100903968, author = {Bo Zhu and Zhe Du and Yancen Dai and Tetsuya Kitaguchi and Sebastian Behrens and Burckhard Seelig}, title = {Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets}, journal = {Biosensors}, year = 2023, } @article{CTT100903969, author = {Satoshi Okada and Yuka Muto and Bo Zhu and Hiroshi Ueda and Hiroyuki Nakamura}, title = {Development of a Peptide Sensor Derived from Human ACE2 for Fluorescence Polarization Assays of the SARS-CoV-2 Receptor Binding Domain}, journal = {Analytical Chemistry}, year = 2023, } @article{CTT100877603, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, journal = {Biochemistry}, year = 2023, } @article{CTT100882627, author = {Jiulong Su and Bo Zhu and Akihito Inoue and Hiroyuki Oyama and Izumi Morita and Jinhua Dong and Takanobu Yasuda and Yoshiko Sugita-Konishi and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, journal = {Biosensors and Bioelectronics}, year = 2023, } @article{CTT100882626, author = {B. Zhu and N. Nosaka and S. Kanamaru and J. Dong and Y. Dai and A. Inoue and Y. Yang and K. Kobayashi and T. Kitaguchi and H. Iwasaki and R. Koike and K. Wakabayashi and H. Ueda}, title = {Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents}, journal = {Analyst}, year = 2022, } @article{CTT100882135, author = {Haimei Li and Bo Zhu and Baowei Li and Limei Chen and Xuerao Ning and Hang Dong and Jingru Liang and Xueying Yang and Jinhua Dong and Hiroshi Ueda}, title = {Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors}, journal = {Scientific Reports}, year = 2022, } @article{CTT100880996, author = {Yancen Dai and Yuko Sato and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Kimura and Farid J. Ghadess and Hiroshi Ueda}, title = {Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting}, journal = {Chemical Science}, year = 2022, } @article{CTT100863138, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface}, journal = {Scientific Reports}, year = 2021, } @article{CTT100830108, author = {Almasul Alfi and Bo Zhu and Jasmina Damnjanovic and Takaaki Kojima and Yugo Iwasaki and Hideo Nakano}, title = {Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release}, journal = {Journal of Bioscience and Bioengineering}, year = 2019, } @article{CTT100830109, author = {Takaaki Kojima and Ayako Nakane and Bo Zhu and Almasul Alfi and Hideo Nakano}, title = {A simple, real-time assay of horseradish peroxidase using biolayer interferometry.}, journal = {Bioscience, biotechnology, and biochemistry}, year = 2019, } @article{CTT100830110, author = {Takaaki Kojima and Takuro Mizoguchi and Eri Ota and Jumpei Hata and Keisuke Homma and Bo Zhu and Kiyotaka Hitomi and Hideo Nakano}, title = {Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays.}, journal = {Journal of bioscience and bioengineering}, year = 2015, } @article{CTT100830111, author = {Bo Zhu and Takuro Mizoguchi and Takaaki Kojima and Hideo Nakano}, title = {Ultra-high-throughput screening of an in vitro-synthesized horseradish peroxidase displayed on microbeads using cell sorter.}, journal = {PloS one}, year = 2015, } @article{CTT100830107, author = {Ryoko Ninomiya and Bo Zhu and Takaaki Kojima and Yugo Iwasaki and Hideo Nakano}, title = {Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium}, journal = {Journal of Bioscience and Bioengineering.}, year = 2014, } @article{CTT100830113, author = {Yan Han and Fang Chen and Nuo Li and Bo Zhu and Xianzhen Li}, title = {Bacillus marcorestinctum sp. nov., a novel soil acylhomoserine lactone quorum-sensing signal quenching bacterium.}, journal = {International journal of molecular sciences}, year = 2010, } @inproceedings{CTT100920344, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A bioluminescent immunosensor based on “Trap & Release” of luciferase-derived peptide fused to antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920347, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {Fabrication of a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920345, author = {Cheng Qian and Ayumu Ninomiya and Natsuki Shibukawa and Hiroshi Ueda and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi}, title = {PMBiT: A bioluminescent probe for large antigen detection}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920343, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A one-pot detection system using β-glucuronidase-based enzyme switch and label-free antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100915126, author = {井上暁人 and 朱博 and 安田貴信 and 水谷圭佑 and 小林健 and 北口哲也}, title = {タンパク質言語モデルを用いた一変異効果予測による蛍光免疫センサーの機能向上}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920536, author = {小野義光 and 有坂亮汰 and 二宮歩武 and 柴田貴広 and 内田浩二 and 安田貴信 and 朱博 and 董金華 and 上田宏 and 北口哲也}, title = {ゲノム編集によるオープンサンドイッチ免疫測定用抗体の構築と均一測定系への展開}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920535, author = {Li Tian and Keisuke Yamashita and Takanobu Yasuda and Takashi Tsuboi and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Single fluorescent protein-based biosensor for monitoring Intracellular IP3 dynamics}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100911877, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A customizable one-pot detection system using β-glucuronidase switch and label-free antibody}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915131, author = {渋川 夏希 and 笹本 佳那 and 瀬尾 秀宗 and 安田 貴信 and 朱 博 and 太田 邦史 and 上田 宏 and 北口 哲也}, title = {バイオセンサーに変換可能な抗体の取得に向けた蛍光プローブタンパク質の開発}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915130, author = {安田 貴信 and 朱 博 and 上田 宏 and 北口 哲也}, title = {発光酵素断片のトラップ&リリースに基づく抗原検出法}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915125, author = {Bo Zhu}, title = {Development of diverse immunosensors through rapid molecular evolutions and deep learning}, booktitle = {}, year = 2024, } @inproceedings{CTT100920534, author = {笹本佳那 and 安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {順平行コイルドコイルペプチド型蛍光プローブによる蛍光免疫センサの応答向上}, booktitle = {第12回日本生物工学会東日本支部コロキウム 要旨集}, year = 2024, } @inproceedings{CTT100908478, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {生物発光免疫センサーの発光活性増大機構の解明とその原理の 全長抗体への展開}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920375, author = {Zixu Feng and 相原寛史 and 桜庭俊 and Yanyan Hou and 吉田啓亮 and 安田貴信 and Bo Zhu and 上田宏 and 伊藤寿朗 and 北口哲也}, title = {生細胞イメージングに適用可能なジベレリン蛍光免疫センサーへの改良}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100915124, author = {Bo Zhu}, title = {Molecular and whole-cell immunosensors developed by directed evolution for applications in diagnostics and food-safety control}, booktitle = {}, year = 2023, } @inproceedings{CTT100907587, author = {水谷圭佑 and 小林健 and 朱博 and 井上暁人 and 北口哲也}, title = {教師なしクラスタリングを用いたタンパク質機能予測}, booktitle = {第26回情報論的学習理論ワークショップ (IBIS2023) 要旨集}, year = 2023, } @inproceedings{CTT100911878, author = {Zhu, B. and Qiu, Z. and Mizutani, K. and Kobayashi, K. and Ueda, H. and Kitaguchi, T.}, title = {Rapid development of single fluorescent protein-based immunosensor Flashbody through ultra-high-throughput screening and cell-free protein synthesis}, booktitle = {第75回⽇本⽣物⼯学会⼤会 要旨集}, year = 2023, } @inproceedings{CTT100920359, author = {二宮歩武 and Qian Cheng and 安田貴信 and 朱博 and 北口哲也}, title = {抗原抗体反応が抗体結合タンパク質によって阻害されることを利用した免疫センサーの開発}, booktitle = {第75回日本生物工学会大会 要旨集}, year = 2023, } @inproceedings{CTT100920360, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Fabrication of Nanobody-based Ratiometric Bioluminescent Quenchbody for Simple and Rapid Detection}, booktitle = {第75回日本生物工学会大会 要旨集}, year = 2023, } @inproceedings{CTT100915127, author = {岡田航輝 and 間部悟司 and 加藤晃代 and 朱博 and 北口哲也 and 中野秀雄}, title = {出芽酵母における翻訳促進ペプチドタグに関する研究}, booktitle = {日本農芸化学会中部・関西支部合同大会 要旨集}, year = 2023, } @inproceedings{CTT100908477, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {抗体結合タンパク質を利用した全長抗体の生物発光センサー化}, booktitle = {化学工学会第54回秋季大会要旨集}, year = 2023, } @inproceedings{CTT100920323, author = {Hiroshi Ueda and Bo Zhu and Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake.}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100887855, author = {佐藤 里杏人 and 安田 貴信 and 朱 博 and 北口 哲也 and 加藤 直洋 and 上田 宏}, title = {3分割ルシフェラーゼを用いた免疫測定法における相互作用の測定と数理モデルの作製}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887891, author = {井上暁人 and 安田貴信 and 朱博 and 北口哲也 and 村上明一 and 上田宏}, title = {酵母細胞表層提示系を用いたナノ抗体由来”nano Q-body”のTrp変異進化}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887853, author = {塘 知子 and 朱 博 and 北口 哲也 and 上田 宏}, title = {蛍光免疫センサーQuenchbodyのシグナル増幅と高速スクリーニング法の探索}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100899561, author = {上田宏 and 蘇九龍 and 朱博 and 井上暁人 and 大山浩之 and 森田いずみ and 董金華 and 小西良子 and 北口哲也 and 小林典裕 and 三宅司郎}, title = {各種食中毒から生活者を守る「パトロール酵母」の開発}, booktitle = {第74回日本生物工学会大会要旨集}, year = 2022, } @inproceedings{CTT100899571, author = {Hiroshi Ueda and Bo Zhu and Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake.}, title = {Development of “patrol yeasts” for the detection of multiple toxic substances in foods}, booktitle = {The 15th Asian Congress on Biotechnology}, year = 2022, } @inproceedings{CTT100887888, author = {Hiroshi Ueda and Bo Zhu and Jinhua Dong}, title = {Isolation of recombinant human antibody for the prevention and rapid diagnosis of COVID-19}, booktitle = {}, year = 2022, } @inproceedings{CTT100920373, author = {上田 宏 and DAI YANCEN and 佐藤 優子 and 朱 博 and 北口 哲也 and 木村 宏 and Farid J. Ghadessy}, title = {生細胞内タンパク質の動態観察とそれによる細胞分別が可能な蛍光抗体プローブIntra Q-bodyの構築}, booktitle = {第16回バイオ関連化学シンポジウム 要旨集}, year = 2022, } @inproceedings{CTT100887890, author = {井上暁人 and 鈴木大凱 and YANG Yinghui and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {コルチゾール認識ナノ抗体の蛍光免疫センサー化}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100887859, author = {朱博 and 野坂 宜之 and 董 金華 and 北口 哲也 and 小池 竜 司 and 若林 健二 and 上田 宏}, title = {Converting antibodies to rapid response SARS-CoV-2 homogeneous immunosensors via Quenchbody technology and crowding agent}, booktitle = {化学工学会第53 回秋季大会要旨集}, year = 2022, } @inproceedings{CTT100877602, author = {Bo Zhu and Haimei Li and Yancen Dai and Akihito Inoue and Yinghui Yang and Kaori Kobayashi and Tetsuya Kitaguchi and Jinhua Dong and Hiroshi Ueda}, title = {Rapid construction of homogeneous immunosensors for SARS-CoV-2 nucleocapsid and spike proteins using Quenchbody and Quenchprobe technology}, booktitle = {Bioanalytical Sensors Gordon Research Conference}, year = 2022, } @inproceedings{CTT100920352, author = {Cheng Qian and Bo Zhu and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Development of a thermostable beta-glucuronidase enzyme switch by introducing mutations that weaken interactions between diagonal subunits}, booktitle = {2022年度生物工学若手研究者の集い 要旨集}, year = 2022, } @inproceedings{CTT100875235, author = {Yancen Dai and Bo Zhu and Yuko Sato and Hiroshi Kimura and Farid J. Ghadessy and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of an immunofluorescence probe Q-body for the direct visualization of tumor marker p53 in fixed and living cells.}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875232, author = {椿凜果 and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {蛍光免疫センサーCoiled Quenchbodyのがん光線力学療法への応用}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875230, author = {小林香央里 and 朱博 and 北口哲也 and 上田宏}, title = {蛍光免疫プローブQuenchprobeのダブルラベル化の検討}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875159, author = {朱博 and 董金華 and 寧雪じょう and 井上暁人 and 北口哲也 and 上田宏}, title = {Quenchbodyを用いた1分間SARS-CoV-2及び変異株の検出法}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100854635, author = {Zhu Bo and Dong Jinhua and Xuerao Ning and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of quenchbody-based 1-minute SARS-CoV-2 detection assay.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @misc{CTT100920301, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {BRET nano Q-body: a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, year = 2024, } @misc{CTT100915123, author = {朱博 and 安田貴信 and 北口哲也}, title = {切らずに開く酵素スイッチ}, year = 2024, } @misc{CTT100920300, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: a homogeneous detection system using split β-glucuronidase and label-free antibody}, year = 2023, } @misc{CTT100920203, author = {Bo Zhu and Zhe Du and Yancen Dai and Tetsuya Kitaguchi and Sebastian Behrens and Burckhard Seelig}, title = {Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets}, year = 2023, } @misc{CTT100887850, author = {Abdul Qawee Rani and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering}, year = 2023, } @misc{CTT100899550, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, year = 2022, } @misc{CTT100875117, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface.}, year = 2021, }