@book{CTT100830112, author = {KOJIMA Takaaki and ZHU Bo and NAKANO Hideo}, title = {Construction of a DNA Library on Microbeads Using Whole Genome Amplification.}, publisher = {}, year = 2015, } @article{CTT100831162, author = {Bo Zhu and Rui Gan and Maria D. Cabezas and Takaaki Kojima and Robert Nicol and Michael C. Jewett and Hideo Nakano}, title = {Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins}, journal = {Biotechnology and Bioengineering}, year = 2020, } @article{CTT100908475, author = {Kana Sasamoto and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M}, journal = {The Analyst}, year = 2023, } @article{CTT100903968, author = {Bo Zhu and Zhe Du and Yancen Dai and Tetsuya Kitaguchi and Sebastian Behrens and Burckhard Seelig}, title = {Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets}, journal = {Biosensors}, year = 2023, } @article{CTT100903969, author = {Satoshi Okada and Yuka Muto and Bo Zhu and Hiroshi Ueda and Hiroyuki Nakamura}, title = {Development of a Peptide Sensor Derived from Human ACE2 for Fluorescence Polarization Assays of the SARS-CoV-2 Receptor Binding Domain}, journal = {Analytical Chemistry}, year = 2023, } @article{CTT100877603, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, journal = {Biochemistry}, year = 2023, } @article{CTT100882626, author = {B. Zhu and N. Nosaka and S. Kanamaru and J. Dong and Y. Dai and A. Inoue and Y. Yang and K. Kobayashi and T. Kitaguchi and H. Iwasaki and R. Koike and K. Wakabayashi and H. Ueda}, title = {Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents}, journal = {Analyst}, year = 2022, } @article{CTT100882627, author = {Jiulong Su and Bo Zhu and Akihito Inoue and Hiroyuki Oyama and Izumi Morita and Jinhua Dong and Takanobu Yasuda and Yoshiko Sugita-Konishi and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, journal = {Biosensors and Bioelectronics}, year = 2022, } @article{CTT100882135, author = {Haimei Li and Bo Zhu and Baowei Li and Limei Chen and Xuerao Ning and Hang Dong and Jingru Liang and Xueying Yang and Jinhua Dong and Hiroshi Ueda}, title = {Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors}, journal = {Scientific Reports}, year = 2022, } @article{CTT100880996, author = {Yancen Dai and Yuko Sato and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Kimura and Farid J. Ghadess and Hiroshi Ueda}, title = {Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting}, journal = {Chemical Science}, year = 2022, } @article{CTT100863138, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface}, journal = {Scientific Reports}, year = 2021, } @article{CTT100830108, author = {Almasul Alfi and Bo Zhu and Jasmina Damnjanovic and Takaaki Kojima and Yugo Iwasaki and Hideo Nakano}, title = {Production of active manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release}, journal = {Journal of Bioscience and Bioengineering}, year = 2019, } @article{CTT100830109, author = {Takaaki Kojima and Ayako Nakane and Bo Zhu and Almasul Alfi and Hideo Nakano}, title = {A simple, real-time assay of horseradish peroxidase using biolayer interferometry.}, journal = {Bioscience, biotechnology, and biochemistry}, year = 2019, } @article{CTT100830110, author = {Takaaki Kojima and Takuro Mizoguchi and Eri Ota and Jumpei Hata and Keisuke Homma and Bo Zhu and Kiyotaka Hitomi and Hideo Nakano}, title = {Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays.}, journal = {Journal of bioscience and bioengineering}, year = 2015, } @article{CTT100830111, author = {Bo Zhu and Takuro Mizoguchi and Takaaki Kojima and Hideo Nakano}, title = {Ultra-high-throughput screening of an in vitro-synthesized horseradish peroxidase displayed on microbeads using cell sorter.}, journal = {PloS one}, year = 2015, } @article{CTT100830107, author = {Ryoko Ninomiya and Bo Zhu and Takaaki Kojima and Yugo Iwasaki and Hideo Nakano}, title = {Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium}, journal = {Journal of Bioscience and Bioengineering.}, year = 2014, } @article{CTT100830113, author = {Yan Han and Fang Chen and Nuo Li and Bo Zhu and Xianzhen Li}, title = {Bacillus marcorestinctum sp. nov., a novel soil acylhomoserine lactone quorum-sensing signal quenching bacterium.}, journal = {International journal of molecular sciences}, year = 2010, } @inproceedings{CTT100911877, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A customizable one-pot detection system using β-glucuronidase switch and label-free antibody}, booktitle = {}, year = 2024, } @inproceedings{CTT100915130, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {発光酵素断片のトラップ&リリースに基づく抗原検出法}, booktitle = {}, year = 2024, } @inproceedings{CTT100915131, author = {Natsuki Shibukawa and Kana Sasamoto and 瀬尾 秀宗 and Takanobu Yasuda and Bo Zhu and 太田 邦史 and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {バイオセンサーに変換可能な抗体の取得に向けた蛍光プローブタンパク質の開発}, booktitle = {}, year = 2024, } @inproceedings{CTT100915126, author = {Akihito Inoue and Bo Zhu and Takanobu Yasuda and Keisuke Mizutani and Ken Kobayashi and Tetsuya Kitaguchi}, title = {タンパク質言語モデルを用いた一変異効果予測による蛍光免疫センサーの機能向上}, booktitle = {}, year = 2024, } @inproceedings{CTT100915125, author = {Bo Zhu}, title = {Development of diverse immunosensors through rapid molecular evolutions and deep learning}, booktitle = {}, year = 2024, } @inproceedings{CTT100915124, author = {Bo Zhu}, title = {Molecular and whole-cell immunosensors developed by directed evolution for applications in diagnostics and food-safety control}, booktitle = {}, year = 2023, } @inproceedings{CTT100908478, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {生物発光免疫センサーの発光活性増大機構の解明とその原理の 全長抗体への展開}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100915127, author = {岡田航輝 and 間部悟司 and 加藤晃代 and Bo Zhu and Tetsuya Kitaguchi and 中野秀雄}, title = {出芽酵母における翻訳促進ペプチドタグに関する研究}, booktitle = {}, year = 2023, } @inproceedings{CTT100907587, author = {Keisuke Mizutani and Ken Kobayashi and Bo Zhu and Tetsuya Kitaguchi and Akihito Inoue}, title = {教師なしクラスタリングを用いたタンパク質機能予測}, booktitle = {}, year = 2023, } @inproceedings{CTT100911878, author = {Zhu, B. and Qiu, Z. and Mizutani, K. and Kobayashi, K. and Ueda, H. and Kitaguchi, T.}, title = {Rapid development of single fluorescent protein-based immunosensor Flashbody through ultra-high-throughput screening and cell-free protein synthesis}, booktitle = {}, year = 2023, } @inproceedings{CTT100908477, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {抗体結合タンパク質を利用した全長抗体の生物発光センサー化}, booktitle = {化学工学会第54回秋季大会要旨集}, year = 2023, } @inproceedings{CTT100887855, author = {Rianto Sato and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Naohiro Kato and Hiroshi Ueda}, title = {3分割ルシフェラーゼを用いた免疫測定法における相互作用の測定と数理モデルの作製}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887891, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and 村上明一 and Hiroshi Ueda}, title = {酵母細胞表層提示系を用いたナノ抗体由来”nano Q-body”のTrp変異進化}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887853, author = {Tomoko Tsutsumi and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {蛍光免疫センサーQuenchbodyのシグナル増幅と高速スクリーニング法の探索}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100899561, author = {Hiroshi Ueda and Jiulong Su and Bo Zhu and Akihito Inoue and 大山浩之 and 森田いずみ and Jinhua Dong and 小西良子 and Tetsuya Kitaguchi and 小林典裕 and 三宅司郎}, title = {各種食中毒から生活者を守る「パトロール酵母」の開発}, booktitle = {第74回日本生物工学会大会要旨集}, year = 2022, } @inproceedings{CTT100887888, author = {Hiroshi Ueda and Bo Zhu and Jinhua Dong}, title = {Isolation of recombinant human antibody for the prevention and rapid diagnosis of COVID-19}, booktitle = {}, year = 2022, } @inproceedings{CTT100899571, author = {Hiroshi Ueda and Bo Zhu and Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake.}, title = {Development of “patrol yeasts” for the detection of multiple toxic substances in foods}, booktitle = {The 15th Asian Congress on Biotechnology}, year = 2022, } @inproceedings{CTT100887859, author = {Bo Zhu and 野坂 宜之 and Jinhua Dong and Tetsuya Kitaguchi and 小池 竜 司 and 若林 健二 and Hiroshi Ueda}, title = {Converting antibodies to rapid response SARS-CoV-2 homogeneous immunosensors via Quenchbody technology and crowding agent}, booktitle = {化学工学会第53 回秋季大会要旨集}, year = 2022, } @inproceedings{CTT100887890, author = {Akihito Inoue and Taiga Suzuki and Yinghui Yang and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {コルチゾール認識ナノ抗体の蛍光免疫センサー化}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100887854, author = {Dai Yancen and Sato Yuko and Zhu Bo and Kitaguchi Tetsuya and Kimura Hiroshi and Farid J. Ghadessy and Ueda Hiroshi}, title = {Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live-cell imaging and sorting}, booktitle = {Bioanalytical Sensors Gordon Research Conference}, year = 2022, } @inproceedings{CTT100877602, author = {Bo Zhu and Haimei Li and Yancen Dai and Akihito Inoue and Yinghui Yang and Kaori Kobayashi and Tetsuya Kitaguchi and Jinhua Dong and Hiroshi Ueda}, title = {Rapid construction of homogeneous immunosensors for SARS-CoV-2 nucleocapsid and spike proteins using Quenchbody and Quenchprobe technology}, booktitle = {Bioanalytical Sensors Gordon Research Conference}, year = 2022, } @inproceedings{CTT100875230, author = {Kaori Kobayashi and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {蛍光免疫プローブQuenchprobeのダブルラベル化の検討}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875235, author = {Yancen Dai and Bo Zhu and Yuko Sato and Hiroshi Kimura and Farid J. Ghadessy and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of an immunofluorescence probe Q-body for the direct visualization of tumor marker p53 in fixed and living cells.}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875232, author = {Rika Tsubaki and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {蛍光免疫センサーCoiled Quenchbodyのがん光線力学療法への応用}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875159, author = {Bo Zhu and Jinhua Dong and 寧雪じょう and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Quenchbodyを用いた1分間SARS-CoV-2及び変異株の検出法}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100854635, author = {Zhu Bo and Dong Jinhua and Xuerao Ning and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of quenchbody-based 1-minute SARS-CoV-2 detection assay.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @misc{CTT100915123, author = {Bo Zhu and Takanobu Yasuda and Tetsuya Kitaguchi}, title = {切らずに開く酵素スイッチ}, year = 2024, } @misc{CTT100887850, author = {Abdul Qawee Rani and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering}, year = 2023, } @misc{CTT100899550, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, year = 2022, } @misc{CTT100875117, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface.}, year = 2021, }