@book{CTT100853162, author = {Marie Mita and Devina Wongso and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi}, title = {Development of a single fluorescent protein-based green glucose indicator by semirational molecular design and molecular evolution.}, publisher = {Springer}, year = 2021, } @book{CTT100759574, author = {董 金華 and 上田 宏}, title = {転写因子IRF4の欠失によるCD4+ T細胞の機能不全が移植受容を促進する}, publisher = {羊土社}, year = 2018, } @book{CTT100754211, author = {Jinhua Dong and Hiroshi Ueda}, title = {Open sandwich immunoassay and its applications}, publisher = {Nova Science Publishers Inc., New York}, year = 2017, } @book{CTT100685495, author = {生物化学的測定研究会 and 上田宏 and 小林典裕 and 三宅司郎 and 荒川秀俊(編)}, title = {免疫測定法 基礎から先端まで}, publisher = {講談社}, year = 2014, } @article{CTT100926938, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Mitsue Nagamine and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody}, journal = {Biosensors and Bioelectronics}, year = 2024, } @article{CTT100908475, author = {Kana Sasamoto and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M}, journal = {The Analyst}, year = 2023, } @article{CTT100920197, author = {Sohei Hiasa and Takeru Fujimori and Saki Aiki and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi}, title = {Development of green fluorescent protein-based cAMP indicators for covering a wide range of cAMP concentrations.}, journal = {RSC Advances}, year = 2023, } @article{CTT100908476, author = {Yoshihiro Ito and Ryuichi Sasaki and Sayaka Asari and Takanobu Yasuda and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient Microfluidic Screening Method Using a Fluorescent Immunosensor for Recombinant Protein Secretions}, journal = {Small}, year = 2023, } @article{CTT100903969, author = {Satoshi Okada and Yuka Muto and Bo Zhu and Hiroshi Ueda and Hiroyuki Nakamura}, title = {Development of a Peptide Sensor Derived from Human ACE2 for Fluorescence Polarization Assays of the SARS-CoV-2 Receptor Binding Domain}, journal = {Analytical Chemistry}, year = 2023, } @article{CTT100877603, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, journal = {Biochemistry}, year = 2023, } @article{CTT100882627, author = {Jiulong Su and Bo Zhu and Akihito Inoue and Hiroyuki Oyama and Izumi Morita and Jinhua Dong and Takanobu Yasuda and Yoshiko Sugita-Konishi and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, journal = {Biosensors and Bioelectronics}, year = 2023, } @article{CTT100882626, author = {B. Zhu and N. Nosaka and S. Kanamaru and J. Dong and Y. Dai and A. Inoue and Y. Yang and K. Kobayashi and T. Kitaguchi and H. Iwasaki and R. Koike and K. Wakabayashi and H. Ueda}, title = {Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents}, journal = {Analyst}, year = 2022, } @article{CTT100899545, author = {Mai Takizawa and Yuri Osuga and Rika Ishida and Marie Mita and Kazuki Harada and Hiroshi Ueda and Tetsuya Kitaguchi and Takashi Tsuboi.}, title = {Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging.}, journal = {Commun Biol}, year = 2022, } @article{CTT100882135, author = {Haimei Li and Bo Zhu and Baowei Li and Limei Chen and Xuerao Ning and Hang Dong and Jingru Liang and Xueying Yang and Jinhua Dong and Hiroshi Ueda}, title = {Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors}, journal = {Scientific Reports}, year = 2022, } @article{CTT100880996, author = {Yancen Dai and Yuko Sato and Bo Zhu and Tetsuya Kitaguchi and Hiroshi Kimura and Farid J. Ghadess and Hiroshi Ueda}, title = {Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting}, journal = {Chemical Science}, year = 2022, } @article{CTT100877419, author = {I. Morita and Y. Kiguchi and H. Oyama and K. Yamaki and N. Sakio and Keisuke Kashiwabara and Y. Kuroda and A. Ito and A. Yokota and N. Ikeda and A. Takeuchi and C. Tode and H. Ueda and S. Numazawa and R. Kikura-Hanajiri and T. Yoshida and N. Kobayashi}, title = {Derivatization-assisted immunoassays: application for group-specific detection of potent methamphetamine and amphetamine enantiomers}, journal = {Analytical Methods}, year = 2022, } @article{CTT100877425, author = {H.-O. Yun and H. Ueda and H.-J. Jeong}, title = {Development of a spacer-optimized Quenchbody against tumor necrosis factor alpha}, journal = {Biotechnology annd Bioprocess Engineering}, year = 2022, } @article{CTT100869186, author = {J. Liang and H. Dong and H. Wang and G. Jiang and T. Inagaki and C.E. Gomez-Sanchez and J. Liang and H. Dong and H. Wang and Z. Yi and G. Jiang and T. Inagaki and C.E. Gomez-Sanchez and J. Dong* and H. Ueda*}, title = {Creation of a Quick and Sensitive Fluorescent Immunosensor for Detecting the Mineralocorticoid Steroid Hormone Aldosterone}, journal = {Journal of Steroid Biochemistry and Molecular Biology}, year = 2022, } @article{CTT100867224, author = {Yuki Ohmuro-Matsuyama and Tadaomi Furuta and Hayato Matsui and Masaki Kanai and Hiroshi Ueda}, title = {Miniaturization of bright light-emitting luciferase ALuc: picALuc}, journal = {ACS Chem. Biol.}, year = 2022, } @article{CTT100875114, author = {Marie Mita and Izumi Sugawara and Kazuki Harada and Motoki Ito and Mai Takizawa and Kentaro Ishida and Hiroshi Ueda and Tetsuya Kitaguchi and Takashi Tsuboi.}, title = {Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics}, journal = {Cell Chem Biol}, year = 2022, } @article{CTT100863026, author = {H. Li and X. Li and H. Li and H. Dong and X. Yang and H. Ueda and J. Dong}, title = {Quench-release-based Fluorescent Immunosensor for the Rapid Detection of Tumor Necrosis Factor-alpha}, journal = {ACS Omega}, year = 2021, } @article{CTT100862791, author = {Yuki Ohmuro-Matsuyama and Keiko Gomi and Takuya Shimoda and Hideki Yamaji and Hiroshi Ueda}, title = {Improving the stability of protein–protein interaction assay FlimPIA using a thermostabilized firefly luciferase}, journal = {Frontiers in Bioengineering and Biotechnology}, year = 2021, } @article{CTT100863138, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface}, journal = {Scientific Reports}, year = 2021, } @article{CTT100861229, author = {Shinichi Sato and Masaki Matsumura and Hiroshi Ueda and Hiroyuki Nakamura}, title = {Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region}, journal = {Chemical Communications}, year = 2021, } @article{CTT100858554, author = {Xuerao Ning and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of Fluorescent Immunosensor Quenchbody to Detect His-Tagged Recombinant Proteins Produced in Bioprocess}, journal = {Sensors}, year = 2021, } @article{CTT100858035, author = {T. Yasuda and A. Inoue and T. Kitaguchi and H. Ueda}, title = {Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides}, journal = {Chemical Communications}, year = 2021, } @article{CTT100848804, author = {Y. Liang and Y. Wang and F. Wang and J. Li and C. Wang and J. Dong and H. Ueda and Z. Xiao and Y. Shen and Z. Xu and H. Wang}, title = {An Enhanced Open Sandwich Immunoassay by Molecular Evolution for Noncompetitive Detection of Alternaria Mycotoxin Tenuazonic Acid}, journal = {Food Chemistry}, year = 2021, } @article{CTT100848715, author = {Riho Takahashi and Takanobu Yasuda and Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {BRET Q‐Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase−Dye−Antibody Fusion with Enhanced Response}, journal = {Analytical Chemistry}, year = 2021, } @article{CTT100847093, author = {Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Kimura and Hiroshi Ueda}, title = {Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies}, journal = {ACS Omega}, year = 2021, } @article{CTT100846219, author = {Hiroto Okuyama and Takanori Tamaki and Yuhei Oshiba and Hiroshi Ueda and Takeo Yamaguchi}, title = {Numerical Modeling for Sensitive and Rapid Molecular Detection by Membrane-Based Immunosensors}, journal = {Analytical Chemistry}, year = 2021, } @article{CTT100836504, author = {Akihito Inoue and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q‐body for Rapid Signal-On Detection of Small Hapten Methotrexate}, journal = {ACS Sensors}, year = 2020, } @article{CTT100838310, author = {Yoshitaka HARADA and Yuki OHMURO-MATSUYAMA and Mika TSUNA and Hiroshi UEDA}, title = {An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens}, journal = {Analytical Sciences}, year = 2020, } @article{CTT100837189, author = {Kazuki Harada and Takami Chihara and Yuki Hayasaka and Marie Mita and Mai Takizawa and Kentaro Ishida and Mary Arai and Saki Tsuno and Mitsuharu Matsumoto and Takeshi Ishihara and Hiroshi Ueda and Tetsuya Kitaguchi and Takashi Tsuboi}, title = {Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging.}, journal = {Sci Rep}, year = 2020, } @article{CTT100830361, author = {L. Chen and R. Tan and Y. Zhou and L. Zhang and S. Zhanga and X. Li and Y. Cong and H. Li and P. Sun and L. Chen and R. Tan and Y. Zhou and L. Zhang and S. Zhang and X. Li and Y. Cong and H. Li and P. Sun and H. Ueda and J. Dong}, title = {Development of an Open Sandwich ELISA for the Detection of Microcystin-LR}, journal = {Microchemical Journal}, year = 2020, } @article{CTT100827505, author = {J. Dong and C. Miyake and T. Yasuda and H. Oyama and I. Morita and T. Tsukahara and M. Takahashi and H.-J. Jeong and T. Kitaguchi and N. Kobayashi and H. Ueda}, title = {PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor}, journal = {Biosensors and Bioelectronics}, year = 2020, } @article{CTT100826337, author = {Hee-Jin Jeong and Jinhua Dong and Chang-Hun Yeom and Hiroshi Ueda}, title = {Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine}, journal = {Methods and Protocols}, year = 2020, } @article{CTT100821932, author = {Chungyong Han and Beom K. Choi and Seon-Hee Kim and Su-Jung Sim and Seongeun Han and Bomi Park and Yohei Tsuchiya and Masaki Takahashi and Young H. Kim and Hyeon-Seok Eom and Tetsuya Kitaguchi and Hiroshi Ueda and Byoung S. Kwon}, title = {Polymorphic region-specific antibody for evaluation of affinity-associated profile of chimeric antigen receptor}, journal = {Molecular Therapy: Oncolytics}, year = 2020, } @article{CTT100826198, author = {Shinichi Sato and Masaki Matsumura and Tetsuya Kadonosono and Satoshi Abe and Takafumi Ueno and Hiroshi Ueda and Hiroyuki Nakamura}, title = {Site-selective protein chemical modification of exposed tyrosine residues using tyrosine click reaction}, journal = {Bioconjugate Chemistry}, year = 2020, } @article{CTT100821930, author = {Jinhua Dong and Yuya Oka and Hee-Jin Jeong and Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex}, journal = {Biotechnology and Bioengineering}, year = 2020, } @article{CTT100814312, author = {Kanai, Y. and Ohmuro-Matsuyama, Y. and Tanioku, M. and Ushiba, S. and Ono, T. and Inoue, K. and Kitaguchi, T. and Kimura, M. and Ueda, H. and Matsumoto, K.}, title = {Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens}, journal = {ACS Sensors}, year = 2020, } @article{CTT100814313, author = {Jiulong Su and Tetsuya Kitaguchi and Yuki Ohmuro-Matsuyama and Theresa Seah and Farid J. Ghadessy and Shawn Hoon and Hiroshi Ueda}, title = {Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens}, journal = {Scientific Reports}, year = 2019, } @article{CTT100814310, author = {Y. Cong and H. Dong and X. Wei and L. Zhang and J. Bai and J. Wu and J.X. Huang and Z. Gao and H. Ueda and J. Dong}, title = {A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol}, journal = {Ecotoxicology and Environmental Safety}, year = 2019, } @article{CTT100836860, author = {Chan-I Chung and Yuko Sato and Yuki Ohmuro-Matsuyama and Shinichi Machida and Hitoshi Kurumizaka and Hiroshi Kimura and Hiroshi Ueda}, title = {Intrabody-based FRET probe to visualize endogenous histone acetylation}, journal = {Scientific Reports}, year = 2019, } @article{CTT100799987, author = {Jiulong Su and Cyrus Beh and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Shawn Hoon and Hiroshi Ueda}, title = {Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens}, journal = {Journal of Bioscience and Bioengineering}, year = 2019, } @article{CTT100829107, author = {Renee Dale and Yuki Ohmuro-Matsuyama and Hiroshi Ueda and Naohiro Kato}, title = {Non-steady state analysis of enzyme kinetics in real time elucidates substrate association and dissociation rates: demonstration with analysis of firefly luciferase mutants}, journal = {BIOCHEMISTRY-US}, year = 2019, } @article{CTT100814311, author = {Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {Protein-Protein Interaction Assays Using Split-NanoLuc}, journal = {Bioluminescence - Analytical Applications and Basic Biology}, year = 2019, } @article{CTT100828948, author = {Marie Mita and Motoki Ito and Kazuki Harada and Izumi Sugawara and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi}, title = {Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells}, journal = {Analytical Chemistry}, year = 2019, } @article{CTT100793801, author = {M. Mita and M. Ito and I. Sugawara and H. Ueda and T. Tsuboi and T. Kitaguchi}, title = {Green fluorescent protein-based glucose indicators report glucose dynamics in living cells}, journal = {Analytical Chemistry}, year = 2019, } @article{CTT100785002, author = {Hee-Jin Jeong and Jinhua Dong and Hiroshi Ueda}, title = {Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies}, journal = {Sensors}, year = 2018, } @article{CTT100773664, author = {A. Sasao and M. Takaki and H.-J. Jeong and K. Yonemitsu and Y. Ohtsu and H. Tsutsumi and S. Furukawa and H. Morioka and H. Ueda and Y. Nishitani}, title = {Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor}, journal = {Drug Testing and Analysis}, year = 2018, } @article{CTT100772438, author = {Yuki Ohmuro-Matsuyama and Takahiro Yamashita and Keiko Gomi and Hideki Yamaji and Hiroshi Ueda}, title = {Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage}, journal = {Analytcal Biochemistry}, year = 2018, } @article{CTT100765659, author = {Jinhua Dong and Richi Fujita and Tamotsu Zako and Hiroshi Ueda}, title = {Construction of Quenchbodies to detect and image amyloid β oligomers}, journal = {ANAL BIOCHEM}, year = 2018, } @article{CTT100761172, author = {Jiulong Su and Jinhua Dong and Tetsuya Kitaguchi and Yuki Omuro and Hiroshi Ueda}, title = {Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation}, journal = {Analyst}, year = 2018, } @article{CTT100761387, author = {Shitao Zhao and Jinhua Dong and Hee-Jin Jeong and Koichi Okumura and Hiroshi Ueda}, title = {Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay}, journal = {Analytical and Bioanalytical Chemistry}, year = 2018, } @article{CTT100759296, author = {Yuki Omuro and H. Ueda}, title = {Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation}, journal = {Analytical Chemistry}, year = 2018, } @article{CTT100753653, author = {Hee-Jin Jeong and Takuya Kawamura and Manami Iida and Yumi Kawahigashi and Mutsumi Takigawa and Yuki Ohmuro-Matsuyama and Chan-I Chung and Jinhua Dong and Masuo Kondoh and Hiroshi Ueda}, title = {Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin}, journal = {Analytical Chemistry}, year = 2017, } @article{CTT100789431, author = {Masayoshi Tanaka and Okochi M and Muto M and Yanai K and Onodera T and Wang J and Ueda H and Toko K.}, title = {Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody.}, journal = {ACS combinatorial science}, year = 2017, } @article{CTT100751613, author = {H.-J. Jeong and K. Matsumoto and S. Itayama and K. Kodama and R. Abe and J. Dong and M. Shindo and H. Ueda}, title = {Construction of dye-stapled Quenchbody by photochemical crosslinking to antibody nucleotide-binding site}, journal = {Chemical Communications}, year = 2017, } @article{CTT100745301, author = {Yuki Omuro and Takahiro Yamashita and Lin Huan and Hideki Yamaji and Hiroshi Ueda}, title = {Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity}, journal = {Luminescence}, year = 2017, } @article{CTT100745299, author = {Devina Wongso and Jinhua Dong and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding}, journal = {Analytical Chemistry}, year = 2017, } @article{CTT100737665, author = {Y. Ohmuro and H. Ueda}, title = {Ultrasensitive Firefly luminescent intermediate-based Protein-protein Interaction Assay (FlimPIA) based on the functional complementation of mutant firefly luciferases}, journal = {Methods in Molecular Biology}, year = 2017, } @article{CTT100737666, author = {Hiroto Iwai and Miki Kojima-Misaizu and Jinhua Dong and Hiroshi Ueda}, title = {Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains}, journal = {Methods in Molecular Biology}, year = 2017, } @article{CTT100737453, author = {Jinhua Dong and Hiroshi Ueda}, title = {ELISA-type assays of trace biomarkers using microfluidic methods}, journal = {WIREs Nanomed. Nanobiotechnol.}, year = 2017, } @article{CTT100737267, author = {Hiroshi Ueda}, title = {An Open Sandwich immunoassay for detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic acid}, journal = {Analyst}, year = 2017, } @article{CTT100733965, author = {Hee-Jin Jeong and Tomoki Kojima and Jinhua Dong and Hiroyuki Ohashi and Hiroshi Ueda}, title = {One-pot construction of Quenchbodies using antibody-binding proteins}, journal = {Analytical Methods}, year = 2016, } @article{CTT100718385, author = {Hiroyuki Ohashi and Takashi Matsumoto and Hee-Jin Jeong and Jinhua Dong and Ryoji Abe and Hiroshi Ueda}, title = {Insight into the working mechanism of Quenchbody: Transition of the dye around antibody variable region that fluoresces upon antigen binding}, journal = {Bioconjugate Chemistry}, year = 2016, } @article{CTT100718390, author = {Chan-I Chung and Ryoji Makino and Yuki Ohmuro and Hiroshi Ueda}, title = {Development of a fluorescent protein-antibody FRET probe for the detection and imaging of osteocalcin}, journal = {J. BIOSCI. BIOENG.}, year = 2016, } @article{CTT100735418, author = {上田 宏 and 董 金華 and Hee-Jin Jeong and 阿部 亮二}, title = {ネオバイオ分子としてのQ-body}, journal = {生物工学会誌}, year = 2016, } @article{CTT100737451, author = {Y. Ohmuro and H. Ueda}, title = {A protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases}, journal = {Methods Mol. Biol.}, year = 2016, } @article{CTT100711188, author = {J. Gholami and M. Manteghian and A. Badiei and H. Ueda and M. Javanbakht}, title = {N-butylamine functionalized graphene oxide for detection of Iron(III) by photoluminescence quenching}, journal = {Luminescence}, year = 2016, } @article{CTT100711187, author = {Hiroto Iwai and Miki Kojima-Misaizu and Jinhua Dong and Hiroshi Ueda}, title = {Creation of a ligand-dependent enzyme by fusing circularly permuted antibody variable region domains}, journal = {BIOCONJUGATE CHEM}, year = 2016, } @article{CTT100711186, author = {Renee Dale and Yuki Ohmuro-Matsuyama and Hiroshi Ueda and Naohiro Kato}, title = {Mathematical model of the firefly luciferase complementation assay reveals a non-linear relationship between the detected luminescence and the affinity of the protein pair being analyzed}, journal = {PLOS ONE}, year = 2016, } @article{CTT100715953, author = {Yuki Omuro and Mori, K. and Hamada, H. and Hiroshi Ueda and Yamaji, H.}, title = {Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production.}, journal = {CYTOTECHNOLOGY}, year = 2016, } @article{CTT100711185, author = {Jinhua Dong and Hee-Jin Jeong and Hiroshi Ueda}, title = {Preparation of Quenchbodies by protein transamination reaction}, journal = {J BIOSCI BIOENG}, year = 2016, } @article{CTT100703323, author = {Hee-Jin Jeong and Takuya Kawamura and Jinhua Dong and Hiroshi Ueda}, title = {Qーbodies from recombinant single-chain Fv fragment with better yield and expanded palette of fluorophores}, journal = {ACS Sensors}, year = 2015, } @article{CTT100698631, author = {M. Kurihara and Y. Ohmuro-Matsuyama and K. Ayabe and T. Yamashita and H. Yamaji and H. Ueda}, title = {Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions}, journal = {Biotechnol. J.}, year = 2015, } @article{CTT100698630, author = {M. Komatsu and J. Dong and H. Ueda and R. Arai}, title = {Crystal structure of Fab fragment of an anti-osteocalcin C-terminal peptide antibody KTM219}, journal = {Photon Factory Act. Rep.}, year = 2015, } @article{CTT100711189, author = {J. Gholami and M. Manteghian and A. Badiei and M. Javanbakht and H. Ueda}, title = {Label free detection of vitamin B12 based on fluorescence quenching of graphene oxide nanolayer}, journal = {Fullerenes, Nanotubes and Carbon Nanostructures}, year = 2015, } @article{CTT100690553, author = {Jinhua Dong and Tomoki Kojima and Hiroyuki Ohashi and Hiroshi Ueda}, title = {Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity}, journal = {J BIOSCI BIOENG}, year = 2015, } @article{CTT100685489, author = {H.J. Jeong and S. Itayama and H. Ueda}, title = {A signal-on fluorosensor based on quench-release principle for sensitive detection of antibiotic rapamycin}, journal = {Biosensors}, year = 2015, } @article{CTT100685490, author = {C.I. Chung and R. Makino and J. Dong and H. Ueda}, title = {Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes}, journal = {ANAL CHEM}, year = 2015, } @article{CTT100685494, author = {K. Hayashi and Y. Tomozoe and K. Nagai and K. Matsuba and M. Mitsumori and Y. Hiraishi and T. Matsumura and 上田 宏 and 神谷 典穂}, title = {イムノアッセイを目的とした融合タンパク質試薬の設計と生産プロセスの最適化}, journal = {}, year = 2015, } @article{CTT100685491, author = {H. Veisi and J. Gholami and H. Ueda and P. Mohammadi and M. Noroozi}, title = {Magnetically palladium catalyst stabilized by diaminoglyoxime-functionalized magnetic Fe3O4 nanoparticles as active and reusable catalyst for Suzuki coupling reactions}, journal = {J. Mol. Catal. A: Chemical}, year = 2014, } @article{CTT100674786, author = {Hee-Jin Jeong and Hiroshi Ueda}, title = {Strategy for Making a Superior Quenchbody to Proteins: Effect of the Fluorophore Position}, journal = {Sensors (Basel, Switzerland)}, year = 2014, } @article{CTT100676216, author = {Yoshiyuki Sasajima and Yusuke Kohama and Miki Kojima-Misaizu and Naoya Kurokawa and Yuko Hara and Jinhua Dong and Masaki Ihara and Hiroshi Ueda}, title = {Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases}, journal = {Molecular Biotechnology}, year = 2014, } @article{CTT100669486, author = {R. Abe and Hee-Jin Jeong and D. Arakawa and J.H. Dong and Hiroyuki Ohashi and R. Kaigome and F. Saiki and Kyosuke Yamane and Hiroaki Takagi and H.Ueda}, title = {Ultra Q-bodies : quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence}, journal = {Scientific Reports}, year = 2014, } @article{CTT100665621, author = {Hiroshi Ueda and Yuko Hara and Yuki Ohmuro}, title = {Improved protein-protein interaction assay FlimPIA by the entrapment of luciferase conformation}, journal = {Anal. Chem.}, year = 2013, } @article{CTT100664582, author = {Sharif Hasan and Jinhua Dong and Yuko Hara and Yoshihito Morizane and Futoshi Shibasaki and Hiroshi Ueda}, title = {Protein-based open sandwich immuno-PCR for sensitive detection of small biomarkers}, journal = {Anal. Sci.}, year = 2013, } @article{CTT100664579, author = {Yuki Ohmuro-Matsuyama and Kota Nakano and Aoi Kimura and Keiichi Ayabe and Masaki Ihara and Takeshi Wada and Yuki Ohmuro-Matsuyama and Kota Nakano and Aoi Kimura and Keiichi Ayabe and Masaki Ihara and Takeshi Wada and Hiroshi Ueda}, title = {A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases}, journal = {Anal. Chem.}, year = 2013, } @article{CTT100664583, author = {Yuko Hara and Jinhua Dong and Hiroshi Ueda}, title = {Open-sandwich immunoassay for sensitive and broad-range detection of a shellfish toxin gonyautoxin}, journal = {Anal Chim Acta}, year = 2013, } @article{CTT100664585, author = {Jinhua Dong and Akira Sakurai and Namiko Nomura and Enoch Y. Park and Futoshi Shibasaki and Hiroshi Ueda}, title = {Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus}, journal = {PLOS ONE}, year = 2013, } @article{CTT100664580, author = {Yuki Ohmuro-Matsuyama and Chan-I Chung and Hiroshi Ueda}, title = {Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments}, journal = {BMC Biotechnol.}, year = 2013, } @article{CTT100664584, author = {Chungyong Han and Masaki Ihara and Hiroshi Ueda}, title = {Expression of an antibody-enzyme complex by the L-chain fusion method}, journal = {J. Biosci. Bioeng.}, year = 2013, } @article{CTT100664581, author = {Hee-Jin Jeong and Yuki Ohmuro-Matsuyama and Hiroyuki Ohashi and Fuyuki Ohsawa and Yoshiro Tatsu and Masaki Inagaki and Hiroshi Ueda}, title = {Detection of vimentin serine phosphorylation by multicolor Quenchbodies}, journal = {Biosens. Bioelectron.}, year = 2013, } @article{CTT100664578, author = {Yuichiro Tone and Masahiro Kawahara and Daichi Kawaguchi and Hiroshi Ueda and Teruyuki Nagamune}, title = {Death signalobody: Inducing conditional cell death in response to a specific antigen}, journal = {Hum. Gen. Ther. Methods}, year = 2013, } @article{CTT100664588, author = {Xiaobo Liu and Michael Eichenberger and Yuuichiro Fujioka and Jinhua Dong and Hiroshi Ueda}, title = {Improved detection sensitivity and selectivity attained by Open-Sandwich selection of an anti-estradiol antibody}, journal = {Anal. Sci.}, year = 2012, } @article{CTT100664587, author = {Koichi Minami and Masaki Ihara and Shou Kuroda and Hirohiko Tsuzuki and Hiroshi Ueda}, title = {Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments}, journal = {Bioconj. Chem.}, year = 2012, } @article{CTT100664586, author = {Koichiro Saka and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {Activation of target signal transducers utilizing chimeric receptors with signaling-molecule binding motifs}, journal = {Biotechnol. Bioeng.}, year = 2012, } @article{CTT100664589, author = {Etsuji Kaneko and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {Growth Control of Genetically Modified Cells via Antibody/c-Kit Chimera}, journal = {J. Biosci. Bioeng.}, year = 2012, } @article{CTT100664590, author = {Jinhua Dong and Sharif Hasan and Yuuichiro Fujioka and Hiroshi Ueda}, title = {Detection of small molecule diagnostic markers with phage-based Open-Sandwich Immuno-PCR}, journal = {J. Immunol. Methods}, year = 2012, } @article{CTT100664594, author = {Ryoji Abe and Hiroyuki Ohashi and Issei Iijima and Masaki Ihara and Hiroaki Takagi and Takahiro Hohsaka and Hiroshi Ueda}, title = {“Quenchbodies” : quench-based antibody probes that show antigen-dependent fluorescence}, journal = {J. Am. Chem. Soc.}, year = 2011, } @article{CTT100664592, author = {Miki Kojima and Hiroto Iwai and Jinhua Dong and Shean-Lee Lim and Shigekazu Ito and Koichi Okumura and Masaki Ihara and Hiroshi Ueda}, title = {Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules}, journal = {Bioconj. Chem.}, year = 2011, } @article{CTT100664593, author = {Kamrun Nahar Islam and Masaki Ihara and Jinhua Dong and Noriyuki Kasagi and Toshihiro Mori and Hiroshi Ueda}, title = {Direct construction of an open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4}, journal = {Anal. Chem.}, year = 2011, } @article{CTT100664591, author = {Masahiro Kawahara and Jianhong Chen and Takahiro Sogo and Jinying Teng and Makoto Otsu and Masafumi Onodera and Hiromitsu Nakauchi and Hiroshi Ueda and Teruyuki Nagamune}, title = {Growth promotion of genetically modified hematopoietic progenitors using an antibody/c-Mpl chimera}, journal = {Cytokine}, year = 2011, } @article{CTT100664597, author = {Hiroto Iwai and Bengü Oztürk and Masaki Ihara and Hiroshi Ueda}, title = {Antibody affinity maturation in vitro using unconjugated peptide antigen}, journal = {Protein Eng. Des. Sel.}, year = 2010, } @article{CTT100664601, author = {Takashi Dojima and Takuya Nishina and Tatsuya Kato and Hiroshi Ueda and Enoch Y. Park}, title = {Production and purification of baculovirus surface displaying scFv in Bombyx mori larvae using BmNPV bacmid}, journal = {Biotechnol. Appl. Biochem.}, year = 2010, } @article{CTT100664595, author = {Yoshiyuki Sasajima and Ryohei Iwasaki and Kouhei Tsumoto and Izumi Kumagai and Masaki Ihara and Hiroshi Ueda}, title = {Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells}, journal = {J. Immunol. Methods}, year = 2010, } @article{CTT100664596, author = {Yoshiyuki Ohiro and Hiroshi Ueda and Norio Shibata and Teruyuki Nagamune}, title = {Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology}, journal = {J. Biosci. Bioeng.}, year = 2010, } @article{CTT100664598, author = {Kamrun Nahar Islam and Masaki Ihara and Jinhua Dong and Noriyuki Kasagi and Toshihiro Mori and Hiroshi Ueda}, title = {Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples}, journal = {Bioanalysis}, year = 2010, } @article{CTT100664599, author = {Masaki Ihara and Amane Yoshikawa and Yushu Wu and Hiroko Takahashi and Kazuma Mawatari and Kiyohito Shimura and Kae Sato and Takehiko Kitamori and Hiroshi Ueda}, title = {Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device}, journal = {Lab. Chip}, year = 2010, } @article{CTT100664600, author = {Takashi Dojima and Takuya Nishina and Tatsuya Kato and Hirokazu Yagi and Koichi Kato and Hiroshi Ueda and Enoch Y. Park}, title = {Improved secretion of molecular chaperone-assisted human IgG in silkworm, and no alterations in their N-Linked glycan structures}, journal = {Biotechnol. Prog.}, year = 2010, } @article{CTT100664609, author = {Hiroshi Iwanaga and Hirohiko Tsuzuki and Youichirou Kamiyama and Hiroshi Ueda}, title = {Verification of specific reaction between airborne antigen and immobilized antibody at gas-solid interface}, journal = {Anal. Sci.}, year = 2009, } @article{CTT100664603, author = {Kento Tanaka and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera}, journal = {Biotechnol. Prog.}, year = 2009, } @article{CTT100664613, author = {Takashi Dojima and Takuya Nishina and Tatsuya Kato and Hiroshi Ueda and Enoch Y. Park}, title = {Comparison of the efficiencies of different affinity tags in the purification of a recombinant secretory protein expressed in silkworm larval hemolymph}, journal = {Biotechnol. Bioproc. Eng.}, year = 2009, } @article{CTT100664612, author = {Junhua Dong and Masaki Ihara and Hiroshi Ueda}, title = {Antibody Fab display system that can perform open sandwich ELISA}, journal = {Anal. Biochem.}, year = 2009, } @article{CTT100664611, author = {Masaki Ihara and Tatsuya Suzuki and Norihiro Kobayashi and Jun-ichi Goto and Hiroshi Ueda}, title = {Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol}, journal = {Anal. Chem.}, year = 2009, } @article{CTT100664610, author = {Motoki Ishikiriyama and Takuya Nishina and Tatsuya Kato and Hiroshi Ueda and Enoch Y. Park}, title = {Human Single-chain Antibody Expression in the Hemolymph and Fat Body of Silkworm Larvae and Pupae Using BmNPV Bacmids}, journal = {J. Biosci. Bioeng.}, year = 2009, } @article{CTT100664608, author = {Ryohei Iwasaki and Hiroki Kiuchi and Masaki Ihara and Toshihiro Mori and Masayuki Kawakami and Hiroshi Ueda}, title = {Trans-splicing as a novel method to rapidly produce antibody fusion proteins}, journal = {Biochem. Biophys. Res. Commun.}, year = 2009, } @article{CTT100664607, author = {Wenhai Liu and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {The influence of domain structures on the signal transduction of chimeric receptors derived from the erythropoietin receptor}, journal = {J. Biochem.}, year = 2009, } @article{CTT100664606, author = {Enoch Y. Park and Motoki Ishikiriyama and Takuya Nishina and Tatsuya Kato and Hirokazu Yagi and Koichi Kato and Hiroshi Ueda}, title = {Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure}, journal = {J. Biotechnol.}, year = 2009, } @article{CTT100664605, author = {Toshiya Sakata and Masaki Ihara and Izumi Makino and Yuji Miyahara and Hiroshi Ueda}, title = {Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen}, journal = {Anal. Chem.}, year = 2009, } @article{CTT100664604, author = {Takahiro Sogo and Masahiro Kawahara and Hiroshi Ueda and Makoto Otsu and Masafumi Onodera and Hiromitsu Nakauchi and Teruyuki Nagamune}, title = {T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera}, journal = {Cytokine}, year = 2009, } @article{CTT100664602, author = {Tamotsu Zako and Hiroyasu Nagata and Naofumi Terada and Arata Utsumi and Masafumi Sakono and Masafumi Yohda and Hiroshi Ueda and Kohei Soga and Mizuo Maeda}, title = {Cyclic RGD Peptide-Labeled Upconversion Nanophosphors for Tumor Cell-Targeted Imaging}, journal = {Biochem. Biophys. Res. Commun.}, year = 2009, } @article{CTT100664615, author = {S Takeda and S Tsukiji and H Ueda and T Nagamune}, title = {Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides}, journal = {Org. Biomol. Chem.}, year = 2008, } @article{CTT100664617, author = {Masafumi Sakono and Tamotsu Zako and Hiroshi Ueda and Masafumi Yohda and Mizuo Maeda}, title = {Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin}, journal = {FEBS J.}, year = 2008, } @article{CTT100664618, author = {Wenhai Liu and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {Construction of a fluorescein-responsive chimeric receptor with strict ligand dependency}, journal = {Biotechnol. Bioeng.}, year = 2008, } @article{CTT100664619, author = {Younggiu Lee and Tadao Asami and Isomaro Yamaguchi and Hiroshi Ueda and Yoshihito Suzuki}, title = {A new gibberellin detection system in living cells based on VH/VL interaction of antibody}, journal = {Biochem. Biophys. Res. Commun.}, year = 2008, } @article{CTT100664620, author = {Masahiro Kawahara and Yusuke Shimo and Takahiro Sogo and Azusa Hitomi and Hiroshi Ueda and Teruyuki Nagamune}, title = {Antigen-mediated migration of murine pro-B Ba/F3 cells via an antibody/receptor chimera}, journal = {J. Biotechnol.}, year = 2008, } @article{CTT100664614, author = {Kazutoshi Yoshitake and Shoko Waki and Hiroshi Ueda}, title = {Dimerization-based homogeneous fluorosensor proteins for the detection of specific dsDNA}, journal = {Biosens. Bioelectron.}, year = 2008, } @article{CTT100664616, author = {Takahiro Sogo and Masahiro Kawahara and Kouhei Tsumoto and Izumi Kumagai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera}, journal = {J. Immunol. Methods}, year = 2008, } @article{CTT100664623, author = {Y. Ohiro and H. Ueda and N. Shibata and T. Nagamune}, title = {Enhanced fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab'-based immunoconjugates}, journal = {Anal. Biochem.}, year = 2007, } @article{CTT100664621, author = {Tatsuya Suzuki and Yuriko Munakata and Kazuki Morita and Tatsuya Shinoda and Hiroshi Ueda}, title = {Sensitive detection of estrogenic mycotoxin zearalenone by open sandwich immunoassay}, journal = {Anal. Sci.}, year = 2007, } @article{CTT100664626, author = {Masahiro Kawahara and Toshiaki Inoue and Xianying Ren and Takahiro Sogo and Hidetoshi Yamada and Motonobu Katoh and Hiroshi Ueda and Mitsuo Oshimura and Teruyuki Nagamune}, title = {Antigen-mediated growth control of hybridoma cells via a human artificial chromosome}, journal = {Biochim. Biophys. Acta}, year = 2007, } @article{CTT100664625, author = {Masahiro Kawahara and Hiroshi Ueda and Kouhei Tsumoto and Izumi Kumagai and Teruyuki Nagamune}, title = {Mimicry of erythropoietin and interleukin-6 signaling by an antibody/cytokine receptor chimera in murine myeloid 32D cells}, journal = {J. Biochem.}, year = 2007, } @article{CTT100664624, author = {Shean-Lee Lim and Hiroko Ichinose and Tatsuya Shinoda and Hiroshi Ueda}, title = {Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay}, journal = {Anal. Chem.}, year = 2007, } @article{CTT100664622, author = {Yoshiaki Sugimura and Hiroshi Ueda and Masatoshi Maki and Kiyotaka Hitomi}, title = {Novel site-specific immobilization of functional protein using a preferred substrate sequence for transglutaminase 2}, journal = {J. Biotechnol.}, year = 2007, } @article{CTT100664630, author = {Masahiro Kawahara and Yuko Ogo and Kouhei Tsumoto and Izumi Kumagai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer}, journal = {Cytotechnology}, year = 2006, } @article{CTT100664628, author = {Yoshiyuki Sasajima and Takahide Aburatani and Kenzo Sakamoto and Hiroshi Ueda}, title = {Detection of protein tyrosine phosphorylation by Open Sandwich fluoroimmunoassay}, journal = {Biotechnol. Prog.}, year = 2006, } @article{CTT100664627, author = {Hidetoshi Yamada and Atsushi Kunisato and Masahiro Kawahara and Candice G. T. Tahimic and Xianying Ren and Hiroshi Ueda and Teruyuki Nagamune and Motonobu Katoh and Toshiaki Inoue and Mitsuo Nishikawa and Mitsuo Oshimura}, title = {Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome}, journal = {J. Hum. Genet.}, year = 2006, } @article{CTT100664629, author = {Kenji Masuda and Kenzo Sakamoto and Miki Kojima and Takahide Aburatani and Takuya Ueda and Hiroshi Ueda}, title = {The role of interface framework residues in determining antibody VH/VL interaction strength and antigen binding affinity}, journal = {FEBS J.}, year = 2006, } @article{CTT100664631, author = {Miki Kojima and Keiichi Ayabe and Hiroshi Ueda}, title = {Importance of terminal residues on circularly permutated Escherichia coli alkaline phosphatase with high specific activity}, journal = {J. Biosci. Bioeng.}, year = 2005, } @article{CTT100664632, author = {Keiichi Ayabe and Tamotsu Zako and Hiroshi Ueda}, title = {The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps}, journal = {FEBS Lett.}, year = 2005, } @article{CTT100664640, author = {M. Kawahara and Y. Ogo and H. Ueda and T. Nagamune}, title = {Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain}, journal = {Protein Eng. Des. Sel.}, year = 2004, } @article{CTT100664639, author = {M. Kawahara and H. Ueda and K. Tsumoto and I. Kumagai and T. Nagamune}, title = {AMEGA: Antigen-MEdiated Genetically modified cell Amplification}, journal = {J. Immunol. Methods}, year = 2004, } @article{CTT100664638, author = {Naoto Komiya and Hiroshi Ueda and Yoshiyuki Ohiro and Teruyuki Nagamune}, title = {Homogeneous sandwich immunoassay based on the enzymatic complementation induced by single-chain Fv fragments}, journal = {Anal. Biochem.}, year = 2004, } @article{CTT100664637, author = {Mina Okochi and Tomoko Nomura and Tamotsu Zako and Ryo Iizuka and Hiroshi Ueda and Takashi Funatsu and Michel Leroux and Masafumi Yohda}, title = {Kinetics and binding sites for interaction of prefoldin with group II chaperonin: contiguous non-native substrate and chaperonin binding sites in archaeal prefoldin}, journal = {J. Biol. Chem.}, year = 2004, } @article{CTT100664636, author = {Paivi Pihkala and Masahiro Kawahara and Hiroshi Ueda and Teruyuki Nagamune}, title = {An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv}, journal = {Biochem. Biophys. Res. Commun.}, year = 2004, } @article{CTT100664635, author = {Takeshi Takazawa and Noriho Kamiya and Hiroshi Ueda and Teruyuki Nagamune}, title = {Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase}, journal = {Biotechnol. Bioeng.}, year = 2004, } @article{CTT100664634, author = {H. Ueda and P. Kristensen and G. Winter}, title = {Stabilization of antibody VH-domains by proteolytic selection}, journal = {J. Mol. Catal. B:Enzymatic}, year = 2004, } @article{CTT100664633, author = {Bei-Wen Ying and Hideki Taguchi and Hiroshi Ueda and Takuya Ueda}, title = {Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system}, journal = {Biochem. Biophys. Res. Commun.}, year = 2004, } @article{CTT100664642, author = {Masahiro Kawahara and Shinya Ishii and Kouhei Tsumoto and Izumi Kumagai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily}, journal = {Biochem. Pharmacol.}, year = 2004, } @article{CTT100664643, author = {Etsuji Kaneko and Masahiro Kawahara and Kouhei Tsumoto and Izumi Kumagai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Antigen-mediated genetically modified cell amplification (AMEGA) with a single vector transduction}, journal = {J. Chem. Eng. Jpn.}, year = 2004, } @article{CTT100664641, author = {Masahiro Kawahara and Hiroko Kimura and Hiroshi Ueda and Teruyuki Nagamune}, title = {Selection of genetically modified cell population using hapten-specific antibody/receptor chimera}, journal = {Biochem. Biophys. Res. Commun.}, year = 2004, } @article{CTT100664649, author = {Takahide Aburatani and Kenzo Sakamoto and Kenji Masuda and Kosuke Nishi and Hideo Ohkawa and Teruyuki Nagamune and Hiroshi Ueda}, title = {A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens}, journal = {Anal. Chem.}, year = 2003, } @article{CTT100664648, author = {Noriho Kamiya and Takeshi Takazawa and Tsutomu Tanaka and Hiroshi Ueda and Teruyuki Nagamune}, title = {Site-specific crosslinking of functional proteins by transglutamination}, journal = {Enzyme Microb. Tech.}, year = 2003, } @article{CTT100664647, author = {Masahiro Kawahara and Hiroshi Ueda and Sumiyo Morita and Kouhei Tsumoto and Izumi Kumagai and Teruyuki Nagamune}, title = {Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch}, journal = {Nucleic Acids Res.}, year = 2003, } @article{CTT100664646, author = {Atsushi Kitayama and Hiroaki Yoshizaki and Yoshihiro Ohmiya and Hiroshi Ueda and Teruyuki Nagamune}, title = {Creation of a thermostable firefly luciferase with pH-insensitive luminescent color}, journal = {Photochem. Photobiol.}, year = 2003, } @article{CTT100664645, author = {Hiroshi Ueda and Tomoichi Yokozeki and Ryoichi Arai and Kouhei Tsumoto and Izumi Kumagai and Teruyuki Nagamune}, title = {An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation}, journal = {J. Immunol. Methods}, year = 2003, } @article{CTT100664644, author = {Tamotsu Zako and Keiichi Ayabe and Takahide Aburatani and Noriho Kamiya and Atsushi Kitayama and Hiroshi Ueda and Teruyuki Nagamune}, title = {Luminescent and substrate binding activities of firefly luciferase N-terminal domain}, journal = {Biochim. Biophys. Acta}, year = 2003, } @article{CTT100664655, author = {Takahide Aburatani and Hiroshi Ueda and Teruyuki Nagamune}, title = {Importance of a CDR H3 basal residue in VH/VL interaction of human antibodies}, journal = {J. Biochem.}, year = 2002, } @article{CTT100664650, author = {Tomoichi Yokozeki and Hiroshi Ueda and Ryoichi Arai and Walt Mahoney and Teruyuki Nagamune}, title = {A homogeneous noncompetitive immunoassay for the detection of small haptens}, journal = {Anal. Chem.}, year = 2002, } @article{CTT100664651, author = {Yukari Yamakawa and Hiroshi Ueda and Atsushi Kitayama and Teruyuki Nagamune}, title = {Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase}, journal = {J. Biosci. Bioeng.}, year = 2002, } @article{CTT100664652, author = {Yoshiyuki Ohiro and Ryoichi Arai and Hiroshi Ueda and Teruyuki Nagamune}, title = {A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction}, journal = {Anal. Chem.}, year = 2002, } @article{CTT100664653, author = {M. Kawahara and H. Ueda and K. Tsumoto and I. Kumagai and W. Mahoney and T. Nagamune}, title = {Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera}, journal = {J. Biosci. Bioeng.}, year = 2002, } @article{CTT100664654, author = {Ryoichi Arai and Hideyuki Nakagawa and Atsushi Kitayama and Hiroshi Ueda and Teruyuki Nagamune}, title = {Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein}, journal = {J. Biosci. Bioeng.}, year = 2002, } @article{CTT100664661, author = {Ryoichi Arai and Hideyuki Nakagawa and Kouhei Tsumoto and Walt Mahoney and Izumi Kumagai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer}, journal = {Anal. Biochem.}, year = 2001, } @article{CTT100664656, author = {Tamotsu Zako and K. Harada and T. Mannen and S. Yamaguchi and A. Kitayama and Hiroshi Ueda and Teruyuki Nagamune}, title = {Monitoring the refolding process of immobilized firefly luciferase using a biosensor based on surface plasmon resonance}, journal = {J. Biochem.}, year = 2001, } @article{CTT100664657, author = {Seiji Ura and Hiroshi Ueda and Jun Kazami and Genji Kawano and Teruyuki Nagamune}, title = {Single cell reporter assay using cell surface displayed Vargula luciferase}, journal = {J. Biosci. Bioeng.}, year = 2001, } @article{CTT100664658, author = {Masahiro Kawahara and Hiroshi Ueda and Kouhei Tsumoto and Izumi Kumagai and Walt Mahoney and Teruyuki Nagamune}, title = {A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer}, journal = {J. Biochem.}, year = 2001, } @article{CTT100664660, author = {Ryoichi Arai and Hiroshi Ueda and Atsushi Kitayama and Noriho Kamiya and Teruyuki Nagamune}, title = {Design of the linkers which effectively separate domains of a bifunctional fusion protein}, journal = {Protein Eng.}, year = 2001, } @article{CTT100664659, author = {Masahiro Kawahara and Akito Natsume and Satoshi Terada and Koichi Kato and Kouhei Tsumoto and Izumi Kumagai and Masao Miki and Walt Mahoney and Hiroshi Ueda and Teruyuki Nagamune}, title = {Replacing factor dependency with that for lysozyme: Affordable culture of IL-6 dependent hybridoma by transfecting artificial cell surface receptor}, journal = {Biotechnol. Bioeng.}, year = 2001, } @article{CTT100664665, author = {Ryoichi Arai and Hiroshi Ueda and Kouhei Tsumoto and Walt C. Mahoney and Izumi Kumagai and Teruyuki Nagamune}, title = {Fluorolabeling of antibody variable domains with green fluorescent protein variants: Application to an energy transfer-based homogeneous immunoassay}, journal = {Protein Eng.}, year = 2000, } @article{CTT100664664, author = {Chikako Suzuki and Hiroshi Ueda and Walt Mahoney and Teruyuki Nagamune}, title = {Open Sandwich Enzyme-Linked Immunosorbent Assay for the quantitation of small haptens}, journal = {Anal. Biochem.}, year = 2000, } @article{CTT100664663, author = {Hiroshi Ueda and Masahiro Kawahara and Takahide Aburatani and Kouhei Tsumoto and Kazuo Todokoro and Eiji Suzuki and Hajime Nishimura and Paula A. Schueler and Greg Winter and Walt C. Mahoney and Izumi Kumagai and Teruyuki Nagamune}, title = {Cell-growth control by monomeric antigen; the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor}, journal = {J. Immunol. Methods}, year = 2000, } @article{CTT100664662, author = {T. Zako and H. Deguchi and A. Kitayama and H. Ueda and T. Nagamune}, title = {Refolding of firefly luciferase immobilized on agarose beads}, journal = {J. Biochem.}, year = 2000, } @article{CTT100664666, author = {Hiroshi Ueda and Kazuishi Kubota and Yu Wang and Kouhei Tsumoto and Walt Mahoney and Izumi Kumagai and Teruyuki Nagamune}, title = {Homogeneous non-competitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (Open sandwich FIA)}, journal = {Biotechniques}, year = 1999, } @article{CTT100664667, author = {Chikako Suzuki and Hiroshi Ueda and Kouhei Tsumoto and Walt Mahoney and Izumi Kumagai and Teruyuki Nagamune}, title = {Open Sandwich ELISA with VH- / VL- alkaline phosphatase fusion proteins}, journal = {J. Immunol. Methods}, year = 1999, } @article{CTT100664671, author = {Rhoichi Arai and Hiroshi Ueda and Teruyuki Nagamune}, title = {Construction of a chimeric protein between protein G and fluorescence-enhanced green fluorescent protein, and its application to immunoassay}, journal = {J. Ferment. Bioeng.}, year = 1998, } @article{CTT100664668, author = {上田 宏 and 喜多山 篤 and 鈴木 栄二 and 長棟 輝行}, title = {カテコール2,3-ジオキシゲナーゼ−プロテインGキメラ蛋白質の作製とその免疫測定への応用}, journal = {化学工学論文集}, year = 1998, } @article{CTT100664670, author = {Yon H. Kim and Takehiro Iida and Tetsuo Fujita and Satoshi Terada and Atsushi Kitayama and Hiroshi Ueda and Edward V. Prochownik and Eiji Suzuki}, title = {Establishment of an apoptosis-resistant and growth-controllable cell line by transfecting with inducible antisense c-jun gene}, journal = {Biotechnol. Bioeng.}, year = 1998, } @article{CTT100664669, author = {Eiko Murayama-Kayano and Sadaaki Yoshimatsu and Toshiaki Kayano and Takeshi Nishio and Hiroshi Ueda and Teruyuki Nagamune}, title = {Application of the random amplified polymorphic DNA (RAPD) technique to distinguish species of the red tide phytoplankton Chattonella (Raaphydophyceae)}, journal = {J. Ferment. Bioeng.}, year = 1998, } @article{CTT100664672, author = {深野 一 and 上田 宏 and 鈴木 栄二}, title = {競合PCR法における増幅過程の数値モデル}, journal = {生物工学会誌}, year = 1997, } @article{CTT100664673, author = {Kouhei Tsumoto and Yoshiyuki Nishimiya and Nobuhiro Kasai and Hiroshi Ueda and Teruyuki Nagamune and Kyoko Ogasahara and Katsuhide Yutani and Kenji Tokuhisa and Masaaki Matsushima and Izumi Kumagai}, title = {Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen}, journal = {Protein Eng}, year = 1997, } @article{CTT100664674, author = {S. Terada and Y. Itoh and H. Ueda and E. Suzuki}, title = {Characterization and fed-batch culture of hybridoma overexpressing apoptosis supressing gene bcl-2}, journal = {Cytotechnology}, year = 1997, } @article{CTT100664675, author = {E. Suzuki and S. Terada and H. Ueda and T. Fujita and T. Komatsu and S. Takayama and J. C. Reed}, title = {Establishing appoptosis resistant cell lines for improving protein productivity}, journal = {Cytotechnology}, year = 1997, } @article{CTT100664676, author = {C. Suzuki and H. Ueda and E. Suzuki and T. Nagamune}, title = {Construction and characterization of an anti-hapten ScFv-alkaline phosphatase chimeric protein}, journal = {J. Biochem.}, year = 1997, } @article{CTT100664677, author = {Yumi Maeda and Hiroshi Ueda and Jun Kazami and Genji Kawano and Eiji Suzuki and Teruyuki Nagamune}, title = {Engineering of functional chimeric protein G-Vargula luciferase}, journal = {Anal. Biochem.}, year = 1997, } @article{CTT100664678, author = {Tetsuo Fujita and Satoshi Terada and Katsuyuki Fukuoka and Atsushi Kitayama and Hiroshi Ueda and Eiji Suzuki}, title = {Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene}, journal = {Cytotechnology}, year = 1997, } @article{CTT100664679, author = {高橋 一成 and 上田 宏 and 鈴木 栄二}, title = {動物細胞潅流培養の温度特性}, journal = {化学工学論文集}, year = 1996, } @article{CTT100664680, author = {H. Ueda and K. Tsumoto and K. Kubota and E. Suzuki and T. Nagamune and H. Nishimura and P. A. Schueler and G. Winter and I. Kumagai and W. C. Mahoney}, title = {Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region}, journal = {Nat. Biotechnol.}, year = 1996, } @article{CTT100664681, author = {S. Terada and E. Suzuki and H. Ueda and F. Makishima}, title = {Cytokines involving gp130 in signal transduction suppressed growth of a mouse hybridoma cell line and enhanced its antibody production}, journal = {Cytokine}, year = 1996, } @article{CTT100664682, author = {T. Shindo and H. Ueda and F. Makishima and H. Nishimura and E. Suzuki}, title = {Method for obtaining mutants defective in immunoglobulin m transcription factors}, journal = {Biosci. Biotech. Biochem.}, year = 1996, } @article{CTT100664683, author = {Yumi Maeda and Hiroshi Ueda and Jun Kazami and Genji Kawano and Eiji Suzuki and Teruyuki Nagamune}, title = {Truncation of Vargula luciferase still resulted in retention of luminescent activity}, journal = {J. Biochem.}, year = 1996, } @article{CTT100664684, author = {Y. Maeda and H. Ueda and T. Hara and J. Kazami and G. Kawano and E. Suzuki and T. Nagamune}, title = {Expression of a bifunctional chimeric protein A-Vargula luciferase in mammalian cells}, journal = {Biotechniques}, year = 1996, } @article{CTT100664686, author = {T. Fujita and S. Terada and H. Ueda and E. Suzuki}, title = {Overexpression of Bcl-2 improved survival of COS-1 cells and enhanced transient protein production}, journal = {J. Ferment. Bioeng.}, year = 1996, } @article{CTT100664685, author = {A. Kitayama and T. Achioku and T. Yanagawa and K. Kanou and M. Kikuchi and H. Ueda and E. Suzuki and H. Nishimura and T. Nagamune and Y. Kawakami}, title = {Cloning and characterization of extradiol aromatic ring-cleavage dioxygenases of Pseudomonas aeruginosa JI104}, journal = {J. Ferment. Bioeng.}, year = 1996, } @article{CTT100664689, author = {Y. Kohara and H. Ueda and E. Suzuki}, title = {Enhanced settling of mammalian cells in tanks with inclined plates / simulation by fluid mechanical model and experiment}, journal = {J. Chem. Eng. Japan}, year = 1995, } @article{CTT100664687, author = {Hiroshi Ueda and Masaki Nakanishi and Eiji Suzuki and Teruyuki Nagamune}, title = {Enhancement of mutation frequency with nucleotide triphosphate analogs in PCR random mutagenesis}, journal = {J. Ferment. Bioeng.}, year = 1995, } @article{CTT100664688, author = {T. Shindo and H. Ueda and E. Suzuki and H. Nishimura}, title = {A catechol 2,3-dioxygenase gene as a reporter}, journal = {Biosci. Biotech. Biochem.}, year = 1995, } @article{CTT100664690, author = {Y. Itoh and H. Ueda and E. Suzuki}, title = {Over-expression of bcl-2, apoptosis supressing gene, prolonged viable culture period of hybridoma and enhanced antibody production}, journal = {Biotech. Bioeng.}, year = 1995, } @article{CTT100664691, author = {K. Takahashi and S. Terada and H. Ueda and F. Makishima and E. Suzuki}, title = {Growth-rate supression of cultured mammalian cells enhances protein productivity}, journal = {Cytotechnol.}, year = 1994, } @article{CTT100664692, author = {H. Ueda and M. Kikuchi and S. Yagi and H. Nishimura}, title = {Antigen responsive antibody-receptor kinase chimera}, journal = {Bio/Technol.}, year = 1992, } @article{CTT100664693, author = {T. Shindo and H. Ueda and F. Makishima and E. Suzuki and H. Nishimura}, title = {Efficient selection of µm-mutants from µm-expressing myeloma cells by treatment with ricin A-conjugated anti-µ antibody}, journal = {Somatic Cell and Molecular Genetics}, year = 1992, } @article{CTT100664694, author = {Ueda, H. and M. Kikuchi and A. Hasegawa and S. Yagi and H. Nishimura}, title = {新しい免疫センサーのためのIgM-上皮増殖因子受容体融合タンパク質の試作とそのミエローマ細胞における膜発現}, journal = {化学工学論文集}, year = 1991, } @inproceedings{CTT100920343, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A one-pot detection system using β-glucuronidase-based enzyme switch and label-free antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920344, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A bioluminescent immunosensor based on “Trap & Release” of luciferase-derived peptide fused to antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920347, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {Fabrication of a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920345, author = {Cheng Qian and Ayumu Ninomiya and Natsuki Shibukawa and Hiroshi Ueda and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi}, title = {PMBiT: A bioluminescent probe for large antigen detection}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920535, author = {Li Tian and Keisuke Yamashita and Takanobu Yasuda and Takashi Tsuboi and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Single fluorescent protein-based biosensor for monitoring Intracellular IP3 dynamics}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920536, author = {小野義光 and 有坂亮汰 and 二宮歩武 and 柴田貴広 and 内田浩二 and 安田貴信 and 朱博 and 董金華 and 上田宏 and 北口哲也}, title = {ゲノム編集によるオープンサンドイッチ免疫測定用抗体の構築と均一測定系への展開}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915131, author = {渋川 夏希 and 笹本 佳那 and 瀬尾 秀宗 and 安田 貴信 and 朱 博 and 太田 邦史 and 上田 宏 and 北口 哲也}, title = {バイオセンサーに変換可能な抗体の取得に向けた蛍光プローブタンパク質の開発}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915130, author = {安田 貴信 and 朱 博 and 上田 宏 and 北口 哲也}, title = {発光酵素断片のトラップ&リリースに基づく抗原検出法}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100911877, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A customizable one-pot detection system using β-glucuronidase switch and label-free antibody}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920534, author = {笹本佳那 and 安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {順平行コイルドコイルペプチド型蛍光プローブによる蛍光免疫センサの応答向上}, booktitle = {第12回日本生物工学会東日本支部コロキウム 要旨集}, year = 2024, } @inproceedings{CTT100908478, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {生物発光免疫センサーの発光活性増大機構の解明とその原理の 全長抗体への展開}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920375, author = {Zixu Feng and 相原寛史 and 桜庭俊 and Yanyan Hou and 吉田啓亮 and 安田貴信 and Bo Zhu and 上田宏 and 伊藤寿朗 and 北口哲也}, title = {生細胞イメージングに適用可能なジベレリン蛍光免疫センサーへの改良}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920360, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Fabrication of Nanobody-based Ratiometric Bioluminescent Quenchbody for Simple and Rapid Detection}, booktitle = {第75回日本生物工学会大会 要旨集}, year = 2023, } @inproceedings{CTT100911878, author = {Zhu, B. and Qiu, Z. and Mizutani, K. and Kobayashi, K. and Ueda, H. and Kitaguchi, T.}, title = {Rapid development of single fluorescent protein-based immunosensor Flashbody through ultra-high-throughput screening and cell-free protein synthesis}, booktitle = {第75回⽇本⽣物⼯学会⼤会 要旨集}, year = 2023, } @inproceedings{CTT100908477, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {抗体結合タンパク質を利用した全長抗体の生物発光センサー化}, booktitle = {化学工学会第54回秋季大会要旨集}, year = 2023, } @inproceedings{CTT100899581, author = {大室有紀 and 北口哲也 and 上田宏 and 木村宏}, title = {YFP断片とintrabodyを利用した生細胞内におけるエピジェネティクス変化の観察法構築}, booktitle = {第16回日本エピジェネティクス研究会年会 要旨集}, year = 2023, } @inproceedings{CTT100920330, author = {Kana Sasamoto and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Development of “Coiled Q-probe” that can effectively convert antibodies into fluorescent immunosensors}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100920328, author = {Akihito Inoue and Takanobu Yasuda and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Selection and Trp-mutation evolution of a nanobody-based fluorescent immunosensor "nano Q-body" by yeast surface display}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100920323, author = {Hiroshi Ueda and Bo Zhu and Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake.}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100887855, author = {佐藤 里杏人 and 安田 貴信 and 朱 博 and 北口 哲也 and 加藤 直洋 and 上田 宏}, title = {3分割ルシフェラーゼを用いた免疫測定法における相互作用の測定と数理モデルの作製}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887891, author = {井上暁人 and 安田貴信 and 朱博 and 北口哲也 and 村上明一 and 上田宏}, title = {酵母細胞表層提示系を用いたナノ抗体由来”nano Q-body”のTrp変異進化}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100899577, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {逆並行結合抑制型ペプチドペアによる抗体を蛍光免疫センサに変換するプローブタンパク質Coiled Q-probeの蛍光応答向上}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100899579, author = {Keisuke Yamashita and Takashi Tsuboi and Hiroshi Ueda and Tetsuya Kitaguchi.}, title = {Development of a GFP-based biosensor for visualizing intracellular IP3 dynamics}, booktitle = {第45回日本分子生物学会年会要旨集}, year = 2022, } @inproceedings{CTT100899578, author = {日浅 綜平 and 藤森 武流 and Saki Aiki and 上田 宏 and Takashi Tsuboi and 北口 哲也}, title = {幅広い濃度範囲の検出を可能とする複数の緑色蛍光cAMPセンサーの開発}, booktitle = {第45回日本分子生物学会年会要旨集}, year = 2022, } @inproceedings{CTT100899576, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上明一 and 上田宏}, title = {酵母提示系によるナノ抗体由来の蛍光免疫センサnano Q-bodyの分子進化法の開発}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100887853, author = {塘 知子 and 朱 博 and 北口 哲也 and 上田 宏}, title = {蛍光免疫センサーQuenchbodyのシグナル増幅と高速スクリーニング法の探索}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100899571, author = {Hiroshi Ueda and Bo Zhu and Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake.}, title = {Development of “patrol yeasts” for the detection of multiple toxic substances in foods}, booktitle = {The 15th Asian Congress on Biotechnology}, year = 2022, } @inproceedings{CTT100899561, author = {上田宏 and 蘇九龍 and 朱博 and 井上暁人 and 大山浩之 and 森田いずみ and 董金華 and 小西良子 and 北口哲也 and 小林典裕 and 三宅司郎}, title = {各種食中毒から生活者を守る「パトロール酵母」の開発}, booktitle = {第74回日本生物工学会大会要旨集}, year = 2022, } @inproceedings{CTT100887888, author = {Hiroshi Ueda and Bo Zhu and Jinhua Dong}, title = {Isolation of recombinant human antibody for the prevention and rapid diagnosis of COVID-19}, booktitle = {}, year = 2022, } @inproceedings{CTT100883091, author = {大室(松山)有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {最小サイズの発光酵素「picALuc」の開発とその応用}, booktitle = {}, year = 2022, } @inproceedings{CTT100899575, author = {伊藤良浩 and 佐々木隆一 and 朝里さやか and 北口哲也 and 上田 宏}, title = {微小ドロップレット培養とバイオセンサーを用いたタンパク質高生産株スクリーニング法の開発}, booktitle = {第74回日本生物工学会大会要旨集}, year = 2022, } @inproceedings{CTT100899573, author = {倉田晴生 and 安田貴信 and 北口哲也 and 上田宏}, title = {発光タグを付加したペプチドを用いた免疫センサーの構築}, booktitle = {化学工学会第53 回秋季大会要旨集}, year = 2022, } @inproceedings{CTT100900707, author = {安田貴信 and 笹本佳那 and 北口哲也 and 上田宏}, title = {逆平行結合抑制ペプチドペアを用いたクエンチ抗体の構築と評価}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100887890, author = {井上暁人 and 鈴木大凱 and YANG Yinghui and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {コルチゾール認識ナノ抗体の蛍光免疫センサー化}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100887859, author = {朱博 and 野坂 宜之 and 董 金華 and 北口 哲也 and 小池 竜 司 and 若林 健二 and 上田 宏}, title = {Converting antibodies to rapid response SARS-CoV-2 homogeneous immunosensors via Quenchbody technology and crowding agent}, booktitle = {化学工学会第53 回秋季大会要旨集}, year = 2022, } @inproceedings{CTT100920373, author = {上田 宏 and DAI YANCEN and 佐藤 優子 and 朱 博 and 北口 哲也 and 木村 宏 and Farid J. Ghadessy}, title = {生細胞内タンパク質の動態観察とそれによる細胞分別が可能な蛍光抗体プローブIntra Q-bodyの構築}, booktitle = {第16回バイオ関連化学シンポジウム 要旨集}, year = 2022, } @inproceedings{CTT100877602, author = {Bo Zhu and Haimei Li and Yancen Dai and Akihito Inoue and Yinghui Yang and Kaori Kobayashi and Tetsuya Kitaguchi and Jinhua Dong and Hiroshi Ueda}, title = {Rapid construction of homogeneous immunosensors for SARS-CoV-2 nucleocapsid and spike proteins using Quenchbody and Quenchprobe technology}, booktitle = {Bioanalytical Sensors Gordon Research Conference}, year = 2022, } @inproceedings{CTT100920352, author = {Cheng Qian and Bo Zhu and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Development of a thermostable beta-glucuronidase enzyme switch by introducing mutations that weaken interactions between diagonal subunits}, booktitle = {2022年度生物工学若手研究者の集い 要旨集}, year = 2022, } @inproceedings{CTT100869546, author = {大室有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {発光酵素の特性を利用したタンパク質間相互作用検出系、及び新規小型発光酵素の開発}, booktitle = {}, year = 2022, } @inproceedings{CTT100875227, author = {Marie Mita and Izumi Sugawara and Kazuki Harada and Motoki Ito and Mai Takizawa and Kentaro Ishida and Hiroshi Ueda and Tetsuya Kitaguchi and Takashi Tsuboi.}, title = {Visualization of intracellular glucose metabolism dynamics by red fluorescent protein-based glucose indicators.}, booktitle = {第99回日本生理学会大会要旨集}, year = 2022, } @inproceedings{CTT100875230, author = {小林香央里 and 朱博 and 北口哲也 and 上田宏}, title = {蛍光免疫プローブQuenchprobeのダブルラベル化の検討}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875235, author = {Yancen Dai and Bo Zhu and Yuko Sato and Hiroshi Kimura and Farid J. Ghadessy and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of an immunofluorescence probe Q-body for the direct visualization of tumor marker p53 in fixed and living cells.}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875234, author = {Yanmin Chen and Yancen Dai and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of a multicolor Q-body construction method using Escherichia coli.}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100875232, author = {椿凜果 and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {蛍光免疫センサーCoiled Quenchbodyのがん光線力学療法への応用}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100920349, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Fabrication of BRET Q-body Using Various Antibody Fragments}, booktitle = {第10回日本生物工学会東日本支部コロキウム 要旨集}, year = 2022, } @inproceedings{CTT100875217, author = {Ippei Tsujimura and Hiroshi Ueda and Tetsuya Kitaguchi.}, title = {Development of an antibody-based red fluorescent probe and its characterization.}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875223, author = {安田貴信 and 上野慶行 and 栃尾尚哉 and 八木宏昌 and 北口哲也 and 木川隆則 and 上田宏}, title = {NMRによる新規免疫センサーHibodyの抗原結合に伴う発光活性増大機構の解明}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875219, author = {Sohei Hiasa and Takeru Fujimori and Saki Aiki and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi.}, title = {Development of green fluorescent protein-based indicators visualizing wide-range of cAMP dynamics for live-cell imaging.}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875222, author = {三田真理恵 and 菅原和 and 原田一貴 and 伊藤幹 and 滝澤舞 and 石田賢太郎 and 上田宏 and 北口哲也 and 坪井貴司}, title = {赤色蛍光タンパク質型グルコースセンサーの開発と糖代謝のライブセルイメージング}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875221, author = {本城麻衣 and 相原寛史 and Yanyan Hou and 上田宏 and 伊藤寿朗 and 北口哲也}, title = {抗体を分子認識ドメインとする蛍光タンパク質プローブを用いたジベレリンの定量}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875220, author = {Keisuke Yamashita and Ippei Tsujimura and Takashi Tsuboi and Hiroshi Ueda and Tetsuya Kitaguchi.}, title = {Generation of a genetically-encoded IP3 biosensor based on a green fluorescent protein.}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875216, author = {飯島快斗 and 上野慶行 and 北口哲也 and 上田宏}, title = {発光タグ融合抗体結合タンパク質を用いた新規抗原検出系の構築}, booktitle = {第94回日本生化学会大会要旨集}, year = 2021, } @inproceedings{CTT100875215, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {蛍光免疫センサープローブCoiled Q-probeを用いた各種低分子の検出と,FRET型プローブへの展開}, booktitle = {第94回日本生化学会大会要旨集}, year = 2021, } @inproceedings{CTT100875214, author = {安田貴信 and 井上暁人 and 北口哲也 and 上田宏}, title = {レシオ計測が可能な蛍光免疫センサーFRET Coiled Q-bodyにおけるDonor-Acceptor間距離の検討}, booktitle = {第94回日本生化学会大会要旨集}, year = 2021, } @inproceedings{CTT100864151, author = {大室有紀 and 古田忠臣 and 松井勇人 and 叶井正樹 and 上田宏}, title = {明るい小型発光酵素 "picALuc"}, booktitle = {}, year = 2021, } @inproceedings{CTT100875212, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上 明一 and 上田宏}, title = {酵母提示系を用いたナノ抗体由来の蛍光免疫センサ“mini Q-body”選択法の開発}, booktitle = {第 73回日本生物工学会大会要旨集}, year = 2021, } @inproceedings{CTT100875213, author = {Yancen Dai and Atsushi Izutani and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Intracellular detection of tumor suppressor protein p53 by Q-body technology}, booktitle = {第 73回日本生物工学会大会要旨集}, year = 2021, } @inproceedings{CTT100875155, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上 明一 and 上田宏}, title = {酵母細胞表層提示系を用いた抗ヒト血清アルブミン (HSA)ナノ抗体由来“mini Q-body”の選択}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100875159, author = {朱博 and 董金華 and 寧雪じょう and 井上暁人 and 北口哲也 and 上田宏}, title = {Quenchbodyを用いた1分間SARS-CoV-2及び変異株の検出法}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100875157, author = {パクジュンテ and 有坂亮汰 and 柴田貴広 and 内田浩二 and 董金華 and 北口哲也 and 上田宏}, title = {ゲノム編集法によるオープンサンドイッチ免疫測定用抗体の産生}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100875144, author = {Akihito Inoue and Yuki Ohmuro and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of mini Q-body using the nanobody to detect chemotherapy agent.}, booktitle = {}, year = 2021, } @inproceedings{CTT100875140, author = {Hiroshi Ueda and Jinhua Dong and Takanobu Yasuda and Masaki Takahashi and Kana Sasamoto and Hiroyuki Oyama and Izumi Morita and Hee-Jin Jeong and Tetsuya Kitaguchi and Norihiro Kobayashi.}, title = {PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor.}, booktitle = {}, year = 2021, } @inproceedings{CTT100875142, author = {Akihito Inoue and Yuki Ohmuro and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of mini Q-body using the nanobody to detect chemotherapy agent.}, booktitle = {}, year = 2021, } @inproceedings{CTT100854299, author = {Yuki Ohmuro and Tadaomi Furuta and Hayato Matsui and Masaki Kanai and Hiroshi Ueda}, title = {Development of the smallest luciferase “picALuc” among bright-light emitting luciferases}, booktitle = {}, year = 2021, } @inproceedings{CTT100875130, author = {上田宏 and 有坂亮汰 and 朴俊泰 and 董金華 and 柴田貴広 and 大室有紀 and 北口哲也 and 内田浩二}, title = {ゲノム編集法による酸化脂質類の非競合的免疫測定系の構築}, booktitle = {第74回日本酸化ストレス学会・第21回日本NO学会 合同学術集会要旨集}, year = 2021, } @inproceedings{CTT100854631, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {迅速簡便に高性能な蛍光免疫センサーを構築可能なCoiled Q-probeの創出}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854637, author = {Marie Mita and Mai Takizawa and Rei Nagata and Kazuki Harada and Hiroshi Ueda and Tetsuya Kitaguchi and Takashi Tsuboi.}, title = {Sucralose activates intracellular Ca2+ and cAMP signaling in enteroendocrine L cells.}, booktitle = {第98回日本生理学会大会要旨集}, year = 2021, } @inproceedings{CTT100854635, author = {Zhu Bo and Dong Jinhua and Xuerao Ning and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Development of quenchbody-based 1-minute SARS-CoV-2 detection assay.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854634, author = {Xuerao Ning and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of fluorescent biosensor Q-body for detecting recombinant proteins produced in bioprocess}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854633, author = {Yancen Dai and Atsushi Izutani and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Instant detection of tumor suppressor protein p53 by Q-body technology.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854632, author = {Junqian Wang and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Development of a novel immunosensor system based on antigen-dependent assembly of fluorescent proteins.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854629, author = {Takeru Fujimori and Saki Aiki and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi.}, title = {Development of turn-on type green fluorescent cAMP biosensors applicable to wide range of cAMP dynamics.}, booktitle = {第43回日本分子生物学会年会要旨集}, year = 2020, } @inproceedings{CTT100854630, author = {Jiulong Su and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda.}, title = {Development of patrol yeasts for the detection of toxic substances in foods.}, booktitle = {第43回日本分子生物学会年会要旨集}, year = 2020, } @inproceedings{CTT100854628, author = {Ippei Tsujimura and Hiroshi Aihara and Hiroshi Ueda and Tetsuya Kitaguchi.}, title = {Development of a red fluorescent probe inducing its intensity change by antigen binding.}, booktitle = {第43回日本分子生物学会年会要旨集}, year = 2020, } @inproceedings{CTT100854627, author = {Hiroshi Aihara and Yanyan Hou and Hiroshi Ueda and Toshiro Ito and Tetsuya Kitaguchi.}, title = {Development of a plant hormone probe employing antibody as molecular recognition domain for visualizing intracellular dynamics.}, booktitle = {第43回日本分子生物学会年会要旨集}, year = 2020, } @inproceedings{CTT100854518, author = {上田宏 and 董金華 and 三宅千絢 and 安田貴信 and 髙橋昌樹 and 大山浩之 and 森田いずみ and 北口哲也 and 小林典裕}, title = {抗体を蛍光免疫センサーQ-body 化可能なタンパク質PM Q-probe による各種バイオマーカーの測定}, booktitle = {化学工学会第51回秋季大会要旨集}, year = 2020, } @inproceedings{CTT100854516, author = {Jiulong Su and Yasuyuki Nakamura and Tetsuya Kitaguchi and Jun Ishii and Akihito Kondo and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda.}, title = {Development of patrol yeasts for the detection of toxic substances in foods.}, booktitle = {第93回日本生化学会大会要旨集}, year = 2020, } @inproceedings{CTT100854520, author = {井上暁人 and 安田貴信 and 北口哲也 and 上田宏}, title = {蛍光免疫センサーQ-bodyを提示した酵母細胞の構築と抗原検出}, booktitle = {化学工学会第51回秋季大会要旨集}, year = 2020, } @inproceedings{CTT100854513, author = {Takanobu Yasuda and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Creation and evaluation of immunosensors that enable ratiometric assay by rapid dual-color labeling of antibody fragments.}, booktitle = {第20回日本蛋白質科学会年会要旨集}, year = 2020, } @inproceedings{CTT100854510, author = {Yoshiyuki Ueno and Naoya Tochio and Junqian Wang and Tetsuya Kitaguchi and Takanori Kigawa and Hiroshi Ueda.}, title = {Mechanistic analysis of novel immunosensor Hibody to clarify the antigen-dependent protrusion of tag sequence attached to the antibody N-terminus.}, booktitle = {第20回日本蛋白質科学会年会要旨集}, year = 2020, } @inproceedings{CTT100837740, author = {有坂亮汰 and 北口哲也 and 上田宏}, title = {ハイブリドーマ細胞のゲノム編集によるオープンサンドイッチ免疫測定用人工抗体の生産}, booktitle = {化学工学会第85年会要旨集}, year = 2020, } @inproceedings{CTT100814877, author = {上田 宏}, title = {タンパク質工学的手法による各種蛍光・発光免疫センサーの開発}, booktitle = {電気学会 光・量子デバイス研究会「医療・バイオ応用を目指したナノ構造・ナノ界面」要旨集}, year = 2020, } @inproceedings{CTT100837737, author = {高橋昌樹 and 董金華 and 北口哲也 and 上田宏}, title = {抗体結合タンパク質Mからなる蛍光バイオセンサープローブ"Q-probe"の熱安定化と各種抗原検出への応用}, booktitle = {第42回日本分子生物学会年会要旨集}, year = 2019, } @inproceedings{CTT100837736, author = {相原寛史 and Yanyan Hou and 上田宏 and 伊藤寿朗 and 北口哲也}, title = {生細胞イメージングを目指した遺伝子コード型ジベレリンプローブの開発}, booktitle = {第42回日本分子生物学会年会要旨集}, year = 2019, } @inproceedings{CTT100837735, author = {千原貴美 and 三田真理恵 and 菅原和 and 相木彩希 and 早坂優希 and 原田一貴 and 上田宏 and 北口哲也 and 坪井貴司}, title = {蛍光タンパク質を用いた緑色蛍光輝度変化型乳酸センサーの開発}, booktitle = {第42回日本分子生物学会年会要旨集}, year = 2019, } @inproceedings{CTT100837734, author = {三田真理恵 and 伊藤幹 and 原田一貴 and 菅原和 and 永田怜 and 芦田翼 and 上田宏 and 坪井貴司 and 北口哲也}, title = {緑色蛍光グルコースセンサーが検出する人工甘味料による膵β細胞のグルコース恒常性攪乱}, booktitle = {第42回日本分子生物学会年会要旨集}, year = 2019, } @inproceedings{CTT100837725, author = {Cyrus Beh and Theresa Seah and Jiulong Su and Yuki Ohmuro-Matsuyama and Tetsuya Kitaguchi and Hiroshi Ueda and Shawn Hoon.}, title = {Facile method for creating arrays of lipid-capped elastomer microwells for bioinspired protocellular sensors.}, booktitle = {}, year = 2019, } @inproceedings{CTT100837731, author = {大室有紀 and 北口哲也 and 木村宏 and 上田宏}, title = {YFP断片融合抗体プローブを用いたヒストン翻訳後修飾検出系の構築}, booktitle = {第 71回日本生物工学会大会要旨集}, year = 2019, } @inproceedings{CTT100810593, author = {佐藤伸一 and 松村雅喜 and 上田宏 and 中村浩之}, title = {チロシン残基修飾による抗体の部位選択的修飾と蛍光免疫センサー分子作成法への応用}, booktitle = {}, year = 2019, } @inproceedings{CTT100837723, author = {Jiulong Su and Cyrus Beh and Tetsuya Kitaguchi and Yuki Ohmuro and Theresa Seah and Shawn Hoon and Hiroshi Ueda.}, title = {Creation of a novel sensor enzyme and its integration into protocell for digital detection of various anti-epitope tag antibodies.}, booktitle = {}, year = 2019, } @inproceedings{CTT100837722, author = {上田宏 and 安田貴信 and 上野慶行 and 井上暁人 and 髙橋昌樹 and 大室有紀 and 北口哲也 and 董金華}, title = {Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築}, booktitle = {第 71回日本生物工学会大会要旨集}, year = 2019, } @inproceedings{CTT100799986, author = {上田 宏}, title = {蛋白質工学の基礎技術:蛋白質機能の設計と分子進化}, booktitle = {第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会要旨集}, year = 2019, } @inproceedings{CTT100837726, author = {上野慶行 and 安田貴信 and 大室有紀 and 北口哲也 and 上田宏}, title = {N末にNanoluc由来タグを持つ抗体断片は抗原依存的にNanolucの発光活性を増大させる}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837727, author = {安田貴信 and 北口哲也 and 上田宏}, title = {コイルドコイル形成ペプチドを利用した蛍光ラベル化法による抗体断片の迅速FRETバイオセンサー化}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837728, author = {大室有紀 and 北口哲也 and 木村宏 and 上田宏}, title = {分割YFP融合Intrabodyを用いた生細胞内ヒストンアセチル化検出プローブの構築}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837729, author = {井上暁人 and 大室有紀 and 北口哲也 and 上田宏}, title = {ナノ抗体を用いた抗がん剤センサーmini Q-body の構築}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100799985, author = {上田宏}, title = {ノーベル化学賞を受賞したイノベーター: Gregory Winter博士}, booktitle = {}, year = 2019, } @inproceedings{CTT100771857, author = {Jiulong Su and Juntae Park and Hiroshi Ueda}, title = {Improved stability and lower background signal of sensitive immunosensor system based on mutant β-glucuronidase}, booktitle = {第91回日本生化学会大会要旨集}, year = 2018, } @inproceedings{CTT100771380, author = {高橋 里帆 and 大室 有紀 and 上田 宏}, title = {自ら光るクエンチ抗体BRET Q-bodyの発光特性}, booktitle = {第12回バイオ関連化学シンポジウム講演要旨集}, year = 2018, } @inproceedings{CTT100771381, author = {上田宏 and 大室有紀 and 北口哲也}, title = {均相系測定を可能にする抗原応答性標識抗体の創製}, booktitle = {}, year = 2018, } @inproceedings{CTT100770647, author = {上田宏}, title = {クエンチ抗体による環境中低分子の迅速高感度検出}, booktitle = {}, year = 2018, } @inproceedings{CTT100766829, author = {上田宏}, title = {新規免疫測定法開発におけるBLIの活用例}, booktitle = {日本ポールユーザーミーティング2018}, year = 2018, } @inproceedings{CTT100768650, author = {Hiroshi Ueda and Yuki Ohmuro}, title = {Homogeneous noncompetitive immunological detection of small molecules by ternary luciferase fragment complementation (OS-BLIA)}, booktitle = {}, year = 2018, } @inproceedings{CTT100759297, author = {上田宏}, title = {蛍光消光現象と発光酵素を利用した新原理免疫測定法の開発}, booktitle = {}, year = 2017, } @inproceedings{CTT100759298, author = {上田宏 and 三宅千絢 and 塚原知也 and 高橋昌樹 and 児島智樹 and 鄭熙陳 and 董金華 and 北口哲也 and 大橋広之}, title = {蛍光免疫センサーQ-body迅速構築のための新しい戦略}, booktitle = {化学工学会第49回秋季大会要旨集}, year = 2017, } @inproceedings{CTT100753069, author = {高橋里帆 and 大室有紀 and 上田 宏}, title = {発光酵素融合クエンチ抗体を用いた生物発光抗原検出系の構築}, booktitle = {第69回日本生物工学会大会講演要旨集}, year = 2017, } @inproceedings{CTT100751614, author = {Hiroshi Ueda}, title = {Development of antibody-based imaging probes utilizing fluorescence quenching phenomena}, booktitle = {}, year = 2017, } @inproceedings{CTT100745300, author = {高橋里帆 and 大室有紀 and 上田 宏}, title = {発光酵素融合クエンチ抗体を用いたBRET抗原検出系の構築}, booktitle = {第17回日本蛋白質科学会年会要旨集}, year = 2017, } @inproceedings{CTT100789934, author = {矢内健太郎 and 武藤正記 and Jin Wang and 田中祐圭 and 上田 宏 and 小野寺 武 and 都甲 潔 and 大河内美奈}, title = {爆発物検出に向けた抗体由来の分子認識ペプチドの探索}, booktitle = {}, year = 2017, } @inproceedings{CTT100737020, author = {三宅 千絢 and 鄭 煕陳 and 大室 有紀 and 董 金華 and 上田 宏}, title = {天然抗体をQuenchbody化するための抗体結合蛍光プローブの開発}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100737019, author = {田中 絵美理 and 鍾 蝉伊 and 大室 有紀 and 董 金華 and 上田 宏}, title = {生物発光共鳴エネルギー移動を利用した蛋白質認識抗体プローブの開発}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100737022, author = {大室 有紀 and 上田 宏 and 山地 秀樹}, title = {リチウム添加による昆虫細胞からの抗体分泌生産の向上}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100737021, author = {岡 裕也 and 鄭 煕陳 and 董 金華 and 上田 宏}, title = {蛍光抗体バイオセンサーQuenchbodyを用いたがん細胞検出と治療の試み}, booktitle = {化学工学会第82年会要旨集}, year = 2017, } @inproceedings{CTT100735729, author = {田中絵美理 and 鐘蝉伊 and 大室有紀 and 上田宏}, title = {血清アルブミン検出のための生物発光抗体プローブの開発}, booktitle = {第89回日本生化学会大会要旨集}, year = 2016, } @inproceedings{CTT100734853, author = {武藤正記 and 矢内健太郎 and 田中祐圭 and 上田宏 and Jin Wang and 小野寺武 and 都甲 潔 and 大河内美奈}, title = {抗体相補性決定領域に基づいた揮発性爆発化合物の特異的ペプチドプローブの開発}, booktitle = {}, year = 2016, } @inproceedings{CTT100723781, author = {上田 宏 and 蘇九龍 and 董 金華 and 岩井 宏徒}, title = {人工アロステリック酵素の創出による高感度ホモジニアス免疫測定法の開発}, booktitle = {第68回日本生物工学会大会要旨集}, year = 2016, } @inproceedings{CTT100725464, author = {Hiroshi Ueda and Yuya Oka and Chan-I Chung and Hee-Jin Jeong and Jinhua Dong}, title = {Antibody-based Fluorescent Probes: How Can We Convert Antigen Binding Signal to Fluorescence Signal?}, booktitle = {}, year = 2016, } @inproceedings{CTT100735730, author = {岩井宏徒 and 董金華 and 上田宏}, title = {円順列変異抗体断片Clampbodyを用いた抗原によるβ-ラクタマーゼの活性制御}, booktitle = {第16回日本蛋白質科学会年会要旨集}, year = 2016, } @inproceedings{CTT100734844, author = {Masaki Muto and Kentaro Yanai and Masayoshi Tanaka and Hiroshi Ueda and Takeshi Onodera and Kiyoshi Toko and Mina Okochi}, title = {Construction of peptide probe for detection of volatile explosive compounds}, booktitle = {}, year = 2016, } @inproceedings{CTT100734845, author = {武藤正記 and 矢内健太郎 and Jin Wang and 田中祐圭 and 上田宏 and 小野寺武 and 都甲潔 and 大河内美奈}, title = {爆発物検出センサの開発に向けた抗体アミノ酸配列に基づく認識プローブの設計}, booktitle = {}, year = 2016, } @inproceedings{CTT100734836, author = {矢内健太郎 and 武藤正記 and Jin Wang and 田中祐圭 and 上田宏 and 小野寺武 and 都甲潔 and 大河内美奈}, title = {ペプチドアレイを用いた抗体由来TNT結合ペプチドの配列最適化}, booktitle = {}, year = 2016, } @inproceedings{CTT100734838, author = {武藤正記 and 矢内健太郎 and Jin Wang and 田中祐圭 and 上田 宏 and 小野寺 武 and 都甲 潔 and 大河内美奈}, title = {抗TNT抗体の相補性決定領域に基づいた爆発物認識ペプチドの開発}, booktitle = {}, year = 2016, } @inproceedings{CTT100725463, author = {上田宏}, title = {組み換え抗体を用いた酸化脂質検出系の構築}, booktitle = {}, year = 2016, } @inproceedings{CTT100734834, author = {矢内健太郎 and 武藤正記 and 田中祐圭 and 上田宏 and 大河内美奈}, title = {抗体の相補性決定領域に基づいたTNT結合ペプチドの開発}, booktitle = {}, year = 2016, } @inproceedings{CTT100723803, author = {岡 裕也 and 鄭 熙陳 and 董 金華 and 上田 宏}, title = {蛍光免疫センサーQuenchbodyを用いたがん抗原蛋白質HER2の検出}, booktitle = {化学工学会第81年会要旨集}, year = 2016, } @inproceedings{CTT100723805, author = {Jiulong Su and Jinhua Dong and Hiroshi Ueda}, title = {Development of a novel homogeneous immunoassay using mutant beta-glucuronidase}, booktitle = {化学工学会第81年会要旨集}, year = 2016, } @inproceedings{CTT100724642, author = {Hiroshi Ueda}, title = {Development of biosensor Proteins Utilizing Fluorescence Quenching and Bioluminescent Reactions}, booktitle = {}, year = 2016, } @inproceedings{CTT100724644, author = {上田宏}, title = {組換え抗体と発光酵素を利用した新規測定法の開発}, booktitle = {}, year = 2016, } @inproceedings{CTT100724637, author = {董 金華 and 座古 保 and 上田 宏}, title = {新規免疫測定素子UQ-bodyを用いたアミロイドβオリゴマーの蛍光検出}, booktitle = {BMB2015要旨集}, year = 2015, } @inproceedings{CTT100724633, author = {鄭熙陳 and 松本 健司 and 板山 修也 and 阿部 亮二 and 董金華 and 新藤充 and 上田 宏}, title = {抗体タンパク質からの蛍光免疫センサーQuenchbodyの構築}, booktitle = {BMB2015要旨集}, year = 2015, } @inproceedings{CTT100724639, author = {Hiroshi Ueda and Hee-Jin Jeong and Takuya Kawamura and Manami Iida and Yumi Kawahigashi and Mutsumi Takigawa and Chan-I Chung and Jinhua Dong and Masuo Kondoh}, title = {Development of Quenchbody to image and quantify tumor-associated membrane protein claudin}, booktitle = {}, year = 2015, } @inproceedings{CTT100724638, author = {Hiroshi Ueda and Hideki Yamaji and Yuki Ohmuro-Matsuyama}, title = {Hinge region engineering of firefly luciferases for robust protein-protein interaction assay FlimPIA}, booktitle = {}, year = 2015, } @inproceedings{CTT100724640, author = {Shitao Zhao and Hee-Jin Jeong and Koichi Okumura and Hiroshi Ueda}, title = {Fast and sensitive detection of a neonicotinoid insecticide imidacloprid with a reagentless immunoassay reagent Quenchbody}, booktitle = {}, year = 2015, } @inproceedings{CTT100724641, author = {Chan-I Chung and Yuki Ohmuro-Matsuyama and Yuko Sato and Hiroshi Kimura and Hiroshi Ueda}, title = {Development of intrabody-based FRET probe for monitoring histone H3 acetylation in living cells}, booktitle = {}, year = 2015, } @inproceedings{CTT100724643, author = {大室有紀 and 五味恵子 and 山地秀樹 and 上田宏}, title = {蛋白質間相互作用検出系FlimPIAの実用化を目指したプローブの熱安定性向上}, booktitle = {BMB2015要旨集}, year = 2015, } @inproceedings{CTT100724183, author = {大室有紀 and 五味恵子 and 山地秀樹 and 上田宏}, title = {蛋白質間相互作用検出系FlimPIAにおけるプローブの熱安定性向上}, booktitle = {}, year = 2015, } @inproceedings{CTT100724182, author = {上田宏 and 五味恵子 and 山地秀樹 and 大室有紀}, title = {ヘイケボタル酵素を用いた蛋白質間相互作用検出系FlimPIAの構築}, booktitle = {}, year = 2015, } @inproceedings{CTT100724625, author = {鄭熙陳 and 松本健司 and 板山修也 and 阿部亮二 and 董金華 and 新藤充 and 上田宏}, title = {光化学修飾法によるQuenchbodyの構築}, booktitle = {第9回バイオ関連化学シンポジウム要旨集}, year = 2015, } @inproceedings{CTT100734827, author = {大河内 美奈 and 武藤 正記 and 田中 祐圭 and 上田 宏 and 小野寺 武 and 都甲 潔 and }, title = {TNT抗体の抗原結合部位解析に基づいたペプチドプローブの設計}, booktitle = {}, year = 2015, } @inproceedings{CTT100724630, author = {上田宏}, title = {抗原結合により光る抗体Q-body の開発とそのバイオ計測への応用}, booktitle = {第67回日本生物工学会大会要旨集}, year = 2015, } @inproceedings{CTT100724622, author = {飯田愛未 and 川東 祐美 and 滝川 睦美 and 鍾 蝉伊 and 董金華 and 近藤昌夫 and 上田 宏}, title = {がん関連膜蛋白質クローディン可視化のためのQuenchbodyの開発}, booktitle = {第9回バイオ関連化学シンポジウム要旨集}, year = 2015, } @inproceedings{CTT100695392, author = {上田宏}, title = {Quenchbodies - Quench-based antibody probes that emit antigen-dependent fluorescence}, booktitle = {Immunome Research}, year = 2015, } @inproceedings{CTT100724588, author = {Hiroshi Ueda and Hee-Jin Jeong and Yuki Ohmuro-Matsuyama and Jinhua Dong}, title = {Development of Biosensor Proteins Utilizing Fluorescence Quenching and Bioluminescent Reactions}, booktitle = {Program, 2015 International Symposium & Annual Meeting, KMB}, year = 2015, } @inproceedings{CTT100724616, author = {河村拓哉 and 川東 祐美 and 滝川 睦美 and 鍾 蝉伊 and 董金華 and 近藤昌夫 and 上田 宏}, title = {がん関連膜蛋白質クローディンを認識するQuenchbodyの開発}, booktitle = {第15回日本蛋白質科学会年会要旨集}, year = 2015, } @inproceedings{CTT100725462, author = {上田 宏 and 阿部亮二 and 大橋広行}, title = {夢の免疫測定素子Quenchbody(Q-body)について-食品安全への応用を中心に}, booktitle = {}, year = 2015, } @inproceedings{CTT100724593, author = {董 金華 and ジョンヒジン and 上田 宏}, title = {アミノ転移反応を利用したQuenchbodyの構築}, booktitle = {第15回日本蛋白質科学会年会要旨集}, year = 2015, } @inproceedings{CTT100685488, author = {Hiroshi Ueda and Takahiro Yamashita and Yuki Ohmuro-Matsuyama}, title = {Functional Complementation of Luciferase Half-Reactions: Application to a Robust Protein-Protein Interaction Assay FlimPIA}, booktitle = {}, year = 2014, } @inproceedings{CTT100674610, author = {上田宏 and 大室有紀}, title = {Functional complementation of luciferase half-reactions: application to a robust protein-protein interaction assay FlimPIA}, booktitle = {Luminescence}, year = 2014, } @inproceedings{CTT100692450, author = {Hiroshi Ueda and Naoki Arai and Yuji Tamura and Eizo Miyashita}, title = {An alternative explanation of movement encoding in monkey's primary motor cortex using joint angular velocity and joint torque during reaching tasks}, booktitle = {Neuroscience Research}, year = 2010, } @inproceedings{CTT100692449, author = {Eizo Miyashita and Hiroshi Ueda and Yuki Ueyama}, title = {The monkey primary motor cortex may generate motor commands and estimated arm states during reaching movements}, booktitle = {}, year = 2010, } @misc{CTT100920301, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {BRET nano Q-body: a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, year = 2024, } @misc{CTT100920300, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: a homogeneous detection system using split β-glucuronidase and label-free antibody}, year = 2023, } @misc{CTT100908473, author = {安田 貴信 and 大森 健太 and 上田宏 and 北口哲也}, title = {トラップ&リリースが拓いた免疫センサーの道と抗体薬物複合体の未来}, year = 2023, } @misc{CTT100887850, author = {Abdul Qawee Rani and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering}, year = 2023, } @misc{CTT100899553, author = {伊藤良浩 and 佐々木隆一 and 朝里さやか and 北口哲也 and 上田 宏}, title = {微小ドロップレット培養とバイオセンサーを用いたタンパク質高生産株スクリーニング法の開発}, year = 2022, } @misc{CTT100899552, author = {Sohei Hiasa and Takeru Fujimori and Saki Aiki and Hiroshi Ueda and Takashi Tsuboi and Tetsuya Kitaguchi}, title = {Development of green fluorescent protein-based cAMP indicators with expanded dynamic range and various working ranges}, year = 2022, } @misc{CTT100899550, author = {Bo Zhu and Cheng Qian and Haoxuan Tang and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces}, year = 2022, } @misc{CTT100875117, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface.}, year = 2021, } @misc{CTT100848806, author = {Hiroshi Ueda and Jinhua Dong}, title = {Biosensors: Homogeneous Detection}, year = 2021, } @misc{CTT100861308, author = {上田 宏}, title = {抗体関連タンパク質の蛍光修飾によるバイオセンサー化}, year = 2021, } @misc{CTT100843715, author = {Jinhua Dong and Hiroshi Ueda}, title = {Recent Advances in Quenchbody, a Fluorescent Immunosensor}, year = 2021, } @misc{CTT100780718, author = {上田宏}, title = {2018年ノーベル化学賞を受賞したGregory Winter博士}, year = 2018, } @misc{CTT100769945, author = {Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction}, year = 2018, } @misc{CTT100743320, author = {董 金華 and 上田 宏}, title = {FRETセンサーを用いた生細胞膜電位の定量的測定}, year = 2017, } @misc{CTT100711196, author = {董金華 and 上田宏}, title = {CRISPR/Cas9システムによるマウス・ヒト抗体遺伝子座の編集}, year = 2016, } @misc{CTT100711193, author = {上田宏}, title = {抗原結合により光る抗体Q-bodyの創出}, year = 2016, } @misc{CTT100711192, author = {阿部 亮二 and 上田 宏}, title = {「その場」検出を可能とする光る抗体Q-body}, year = 2016, } @misc{CTT100711194, author = {上田宏}, title = {抗体のセンサー化で生命現象を探る}, year = 2015, } @misc{CTT100711226, author = {Hiroshi Ueda}, title = {Improving Medical Care with Engineering}, year = 2015, } @misc{CTT100711191, author = {董 金華 and 上田 宏}, title = {生物発光タンパク質センサーを用いた薬剤のモニタリング}, year = 2015, } @misc{CTT100711190, author = {阿部 亮二 and 上田 宏}, title = {抗体断片の蛍光技術を用いた迅速・簡便な検査キットの開発}, year = 2015, } @misc{CTT100711195, author = {上田宏}, title = {免疫反応を測れる 光る抗体}, year = 2014, } @misc{CTT100669481, author = {大室有紀 and 上田 宏}, title = {ホタル発光酵素の反応機構を利用したタンパク質間相互作用検出系の開発}, year = 2014, } @misc{CTT100877445, author = {上田宏 and 北口哲也 and SuJiulong and 三宅 司郎  and 小林 典裕 }, title = {抗体断片を含む人工受容体を細胞膜に提示した酵母とこれを用いた抗原検出法 }, howpublished = {公開特許}, year = 2022, month = {}, note = {特願2020-145870(2020/08/31), 特開2022-040923(2022/03/11)} } @misc{CTT100917020, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {登録特許}, year = 2024, month = {}, note = {特願2021-524873(2020/06/03), WO 2020/246495(2020/10/12), 特許第7461661号(2024/03/27)} } @misc{CTT100869590, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/021899(2020/06/03), WO 2020/246495(2020/10/12)} } @misc{CTT100869602, author = {上田宏 and 北口哲也 and 大室有紀 and 上野慶行}, title = {レポータータンパク質断片を付加した組換え抗体を用いるホモジニアス免疫測定法}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/020281(2020/05/22), WO 2020/241486(2020/12/03)} } @misc{CTT100865567, author = {上田宏 and 綱 美香  and 原田 義孝 }, title = {低分子化合物の簡易検出法}, howpublished = {公開特許}, year = 2021, month = {}, note = {特願2020-056188(2020/03/26), 特開2021-156695(2021/10/07)} } @misc{CTT100869583, author = {上田宏 and SuJiulong and 北口哲也 and 大室有紀}, title = {ホモジニアス免疫測定法に適した酵素変異体}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/002264(2020/01/23), WO 2020/162203(2020/08/13)} } @misc{CTT100864142, author = {佐藤伸一 and 中村浩之 and 上田宏}, title = {蛍光標識抗体又は抗体断片}, howpublished = {公開特許}, year = 2021, month = {}, note = {特願2020-534145(2019/07/10), 再表2020/026733(2021/08/19)} } @misc{CTT100831058, author = {上田宏 and SuJiulong and 北口哲也 and 大室有紀 and Shawn Hoon and Cyrus Beh }, title = {バイオセンサー}, howpublished = {登録特許}, year = 2022, month = {}, note = {特願2018-207624(2018/11/02), 特開2020-068746(2020/05/07), 特許第7169582号(2022/11/02)} } @misc{CTT100820743, author = {上田宏 and 大室有紀 and 三宅千絢 and 塚原知也}, title = {抗原検出又は測定用キット}, howpublished = {登録特許}, year = 2021, month = {}, note = {特願2018-566814(2018/01/17), 再表2018-147018(2019/12/12), 特許第6983419号(2021/11/26)} } @misc{CTT100789767, author = {上田宏 and 董金華}, title = {融合タンパク質及びそれを用いた抗原の検出方法}, howpublished = {登録特許}, year = 2021, month = {}, note = {特願2017-563741(2016/12/21), 再表2017/130610(2018/11/15), 特許第6873483号(2021/04/23)} } @misc{CTT100744874, author = {上田宏 and 鄭熙陳 and 董金華 and 阿部 亮二}, title = {抗体のヌクレオチド結合部位(NBS)を利用して蛍光標識された抗体}, howpublished = {公開特許}, year = 2017, month = {}, note = {特願2015-174075(2015/09/03), 特開2017-049184(2017/03/09)} } @misc{CTT100701098, author = {上田宏 and 大室 有紀 and 黒澤 恵子}, title = {ルシフェラーゼ変異体及びそれを用いた分子間相互作用検出方法}, howpublished = {公開特許}, year = 2015, month = {}, note = {特願2015-079444(2015/04/08), 特開2015-211668(2015/11/26)} } @misc{CTT100675219, author = {上田宏 and 大室 有紀 and 和田 猛}, title = {ホタルが光る仕組みを利用した「たんぱく質の出会い」検出技術を開発}, year = 2010, note = {2010-2013}, }