@article{CTT100926938, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Mitsue Nagamine and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody}, journal = {Biosensors and Bioelectronics}, year = 2024, } @article{CTT100908475, author = {Kana Sasamoto and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M}, journal = {The Analyst}, year = 2023, } @article{CTT100908476, author = {Yoshihiro Ito and Ryuichi Sasaki and Sayaka Asari and Takanobu Yasuda and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Efficient Microfluidic Screening Method Using a Fluorescent Immunosensor for Recombinant Protein Secretions}, journal = {Small}, year = 2023, } @article{CTT100882627, author = {Jiulong Su and Bo Zhu and Akihito Inoue and Hiroyuki Oyama and Izumi Morita and Jinhua Dong and Takanobu Yasuda and Yoshiko Sugita-Konishi and Tetsuya Kitaguchi and Norihiro Kobayashi and Shiro Miyake and Hiroshi Ueda}, title = {The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning}, journal = {Biosensors and Bioelectronics}, year = 2023, } @article{CTT100863138, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface}, journal = {Scientific Reports}, year = 2021, } @article{CTT100858554, author = {Xuerao Ning and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Construction of Fluorescent Immunosensor Quenchbody to Detect His-Tagged Recombinant Proteins Produced in Bioprocess}, journal = {Sensors}, year = 2021, } @article{CTT100858035, author = {T. Yasuda and A. Inoue and T. Kitaguchi and H. Ueda}, title = {Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides}, journal = {Chemical Communications}, year = 2021, } @article{CTT100848715, author = {Riho Takahashi and Takanobu Yasuda and Yuki Ohmuro-Matsuyama and Hiroshi Ueda}, title = {BRET Q‐Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase−Dye−Antibody Fusion with Enhanced Response}, journal = {Analytical Chemistry}, year = 2021, } @article{CTT100827505, author = {J. Dong and C. Miyake and T. Yasuda and H. Oyama and I. Morita and T. Tsukahara and M. Takahashi and H.-J. Jeong and T. Kitaguchi and N. Kobayashi and H. Ueda}, title = {PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor}, journal = {Biosensors and Bioelectronics}, year = 2020, } @inproceedings{CTT100920344, author = {Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A bioluminescent immunosensor based on “Trap & Release” of luciferase-derived peptide fused to antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920345, author = {Cheng Qian and Ayumu Ninomiya and Natsuki Shibukawa and Hiroshi Ueda and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi}, title = {PMBiT: A bioluminescent probe for large antigen detection}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920347, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {Fabrication of a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100920343, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A one-pot detection system using β-glucuronidase-based enzyme switch and label-free antibody}, booktitle = {The Joint Congress of 21st International Union for Pure and Applied Biophysics}, year = 2024, } @inproceedings{CTT100915130, author = {安田 貴信 and 朱 博 and 上田 宏 and 北口 哲也}, title = {発光酵素断片のトラップ&リリースに基づく抗原検出法}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100921012, author = {前田侑也 and 安田貴信 and 小野田晃}, title = {トリアゾールカルボアルデヒド基により蛍光色素をN末端に修飾した抗体の調製}, booktitle = {}, year = 2024, } @inproceedings{CTT100920536, author = {小野義光 and 有坂亮汰 and 二宮歩武 and 柴田貴広 and 内田浩二 and 安田貴信 and 朱博 and 董金華 and 上田宏 and 北口哲也}, title = {ゲノム編集によるオープンサンドイッチ免疫測定用抗体の構築と均一測定系への展開}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920535, author = {Li Tian and Keisuke Yamashita and Takanobu Yasuda and Takashi Tsuboi and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Single fluorescent protein-based biosensor for monitoring Intracellular IP3 dynamics}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100911877, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {A customizable one-pot detection system using β-glucuronidase switch and label-free antibody}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915126, author = {井上暁人 and 朱博 and 安田貴信 and 水谷圭佑 and 小林健 and 北口哲也}, title = {タンパク質言語モデルを用いた一変異効果予測による蛍光免疫センサーの機能向上}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100915131, author = {渋川 夏希 and 笹本 佳那 and 瀬尾 秀宗 and 安田 貴信 and 朱 博 and 太田 邦史 and 上田 宏 and 北口 哲也}, title = {バイオセンサーに変換可能な抗体の取得に向けた蛍光プローブタンパク質の開発}, booktitle = {化学工学会第89年会 要旨集}, year = 2024, } @inproceedings{CTT100920534, author = {笹本佳那 and 安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {順平行コイルドコイルペプチド型蛍光プローブによる蛍光免疫センサの応答向上}, booktitle = {第12回日本生物工学会東日本支部コロキウム 要旨集}, year = 2024, } @inproceedings{CTT100908478, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {生物発光免疫センサーの発光活性増大機構の解明とその原理の 全長抗体への展開}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920374, author = {井上暁人 and 安田貴信 and 大内将司 and 北口哲也}, title = {ウサギ抗体を用いたクエンチ型cAMP蛍光免疫センサーの開発}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920375, author = {Zixu Feng and 相原寛史 and 桜庭俊 and Yanyan Hou and 吉田啓亮 and 安田貴信 and Bo Zhu and 上田宏 and 伊藤寿朗 and 北口哲也}, title = {生細胞イメージングに適用可能なジベレリン蛍光免疫センサーへの改良}, booktitle = {第2回日本抗体学会学術大会要旨集}, year = 2023, } @inproceedings{CTT100920360, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Bo Zhu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Fabrication of Nanobody-based Ratiometric Bioluminescent Quenchbody for Simple and Rapid Detection}, booktitle = {第75回日本生物工学会大会 要旨集}, year = 2023, } @inproceedings{CTT100920359, author = {二宮歩武 and Qian Cheng and 安田貴信 and 朱博 and 北口哲也}, title = {抗原抗体反応が抗体結合タンパク質によって阻害されることを利用した免疫センサーの開発}, booktitle = {第75回日本生物工学会大会 要旨集}, year = 2023, } @inproceedings{CTT100908477, author = {安田貴信 and 朱博 and 上田宏 and 北口哲也}, title = {抗体結合タンパク質を利用した全長抗体の生物発光センサー化}, booktitle = {化学工学会第54回秋季大会要旨集}, year = 2023, } @inproceedings{CTT100920330, author = {Kana Sasamoto and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Development of “Coiled Q-probe” that can effectively convert antibodies into fluorescent immunosensors}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100920328, author = {Akihito Inoue and Takanobu Yasuda and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Selection and Trp-mutation evolution of a nanobody-based fluorescent immunosensor "nano Q-body" by yeast surface display}, booktitle = {33rd Anniversary World Congress on Biosensors}, year = 2023, } @inproceedings{CTT100887891, author = {井上暁人 and 安田貴信 and 朱博 and 北口哲也 and 村上明一 and 上田宏}, title = {酵母細胞表層提示系を用いたナノ抗体由来”nano Q-body”のTrp変異進化}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100887855, author = {佐藤 里杏人 and 安田 貴信 and 朱 博 and 北口 哲也 and 加藤 直洋 and 上田 宏}, title = {3分割ルシフェラーゼを用いた免疫測定法における相互作用の測定と数理モデルの作製}, booktitle = {化学工学会第88年会要旨集}, year = 2023, } @inproceedings{CTT100899576, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上明一 and 上田宏}, title = {酵母提示系によるナノ抗体由来の蛍光免疫センサnano Q-bodyの分子進化法の開発}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100899577, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {逆並行結合抑制型ペプチドペアによる抗体を蛍光免疫センサに変換するプローブタンパク質Coiled Q-probeの蛍光応答向上}, booktitle = {第95回日本生化学会大会要旨集}, year = 2022, } @inproceedings{CTT100900707, author = {安田貴信 and 笹本佳那 and 北口哲也 and 上田宏}, title = {逆平行結合抑制ペプチドペアを用いたクエンチ抗体の構築と評価}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100887890, author = {井上暁人 and 鈴木大凱 and YANG Yinghui and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {コルチゾール認識ナノ抗体の蛍光免疫センサー化}, booktitle = {第16回バイオ関連化学シンポジウム要旨集}, year = 2022, } @inproceedings{CTT100899573, author = {倉田晴生 and 安田貴信 and 北口哲也 and 上田宏}, title = {発光タグを付加したペプチドを用いた免疫センサーの構築}, booktitle = {化学工学会第53 回秋季大会要旨集}, year = 2022, } @inproceedings{CTT100875232, author = {椿凜果 and 安田貴信 and 朱博 and 北口哲也 and 上田宏}, title = {蛍光免疫センサーCoiled Quenchbodyのがん光線力学療法への応用}, booktitle = {化学工学会第87年会要旨集}, year = 2022, } @inproceedings{CTT100920349, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Fabrication of BRET Q-body Using Various Antibody Fragments}, booktitle = {第10回日本生物工学会東日本支部コロキウム 要旨集}, year = 2022, } @inproceedings{CTT100875223, author = {安田貴信 and 上野慶行 and 栃尾尚哉 and 八木宏昌 and 北口哲也 and 木川隆則 and 上田宏}, title = {NMRによる新規免疫センサーHibodyの抗原結合に伴う発光活性増大機構の解明}, booktitle = {第44回日本分子生物学会年会要旨集}, year = 2021, } @inproceedings{CTT100875214, author = {安田貴信 and 井上暁人 and 北口哲也 and 上田宏}, title = {レシオ計測が可能な蛍光免疫センサーFRET Coiled Q-bodyにおけるDonor-Acceptor間距離の検討}, booktitle = {第94回日本生化学会大会要旨集}, year = 2021, } @inproceedings{CTT100875215, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {蛍光免疫センサープローブCoiled Q-probeを用いた各種低分子の検出と,FRET型プローブへの展開}, booktitle = {第94回日本生化学会大会要旨集}, year = 2021, } @inproceedings{CTT100875213, author = {Yancen Dai and Atsushi Izutani and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda}, title = {Intracellular detection of tumor suppressor protein p53 by Q-body technology}, booktitle = {第 73回日本生物工学会大会要旨集}, year = 2021, } @inproceedings{CTT100875212, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上 明一 and 上田宏}, title = {酵母提示系を用いたナノ抗体由来の蛍光免疫センサ“mini Q-body”選択法の開発}, booktitle = {第 73回日本生物工学会大会要旨集}, year = 2021, } @inproceedings{CTT100875155, author = {井上暁人 and 安田貴信 and 北口哲也 and 村上 明一 and 上田宏}, title = {酵母細胞表層提示系を用いた抗ヒト血清アルブミン (HSA)ナノ抗体由来“mini Q-body”の選択}, booktitle = {化学工学会第52回秋季大会要旨集}, year = 2021, } @inproceedings{CTT100875140, author = {Hiroshi Ueda and Jinhua Dong and Takanobu Yasuda and Masaki Takahashi and Kana Sasamoto and Hiroyuki Oyama and Izumi Morita and Hee-Jin Jeong and Tetsuya Kitaguchi and Norihiro Kobayashi.}, title = {PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor.}, booktitle = {}, year = 2021, } @inproceedings{CTT100854633, author = {Yancen Dai and Atsushi Izutani and Takanobu Yasuda and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Instant detection of tumor suppressor protein p53 by Q-body technology.}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854631, author = {笹本佳那 and 安田貴信 and 北口哲也 and 上田宏}, title = {迅速簡便に高性能な蛍光免疫センサーを構築可能なCoiled Q-probeの創出}, booktitle = {化学工学会第86年会要旨集}, year = 2021, } @inproceedings{CTT100854520, author = {井上暁人 and 安田貴信 and 北口哲也 and 上田宏}, title = {蛍光免疫センサーQ-bodyを提示した酵母細胞の構築と抗原検出}, booktitle = {化学工学会第51回秋季大会要旨集}, year = 2020, } @inproceedings{CTT100854518, author = {上田宏 and 董金華 and 三宅千絢 and 安田貴信 and 髙橋昌樹 and 大山浩之 and 森田いずみ and 北口哲也 and 小林典裕}, title = {抗体を蛍光免疫センサーQ-body 化可能なタンパク質PM Q-probe による各種バイオマーカーの測定}, booktitle = {化学工学会第51回秋季大会要旨集}, year = 2020, } @inproceedings{CTT100854513, author = {Takanobu Yasuda and Akihito Inoue and Tetsuya Kitaguchi and Hiroshi Ueda.}, title = {Creation and evaluation of immunosensors that enable ratiometric assay by rapid dual-color labeling of antibody fragments.}, booktitle = {第20回日本蛋白質科学会年会要旨集}, year = 2020, } @inproceedings{CTT100837722, author = {上田宏 and 安田貴信 and 上野慶行 and 井上暁人 and 髙橋昌樹 and 大室有紀 and 北口哲也 and 董金華}, title = {Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築}, booktitle = {第 71回日本生物工学会大会要旨集}, year = 2019, } @inproceedings{CTT100837727, author = {安田貴信 and 北口哲也 and 上田宏}, title = {コイルドコイル形成ペプチドを利用した蛍光ラベル化法による抗体断片の迅速FRETバイオセンサー化}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @inproceedings{CTT100837726, author = {上野慶行 and 安田貴信 and 大室有紀 and 北口哲也 and 上田宏}, title = {N末にNanoluc由来タグを持つ抗体断片は抗原依存的にNanolucの発光活性を増大させる}, booktitle = {第19回日本蛋白質科学会年会要旨集}, year = 2019, } @misc{CTT100920301, author = {Yinghui Yang and Akihito Inoue and Takanobu Yasuda and Hiroshi Ueda and Bo Zhu and Tetsuya Kitaguchi}, title = {BRET nano Q-body: a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing}, year = 2024, } @misc{CTT100915123, author = {朱博 and 安田貴信 and 北口哲也}, title = {切らずに開く酵素スイッチ}, year = 2024, } @misc{CTT100908474, author = {安田貴信 and 北口哲也}, title = {互いに引き合う螺旋状ペプチド ~二量体形成から細胞療法まで~}, year = 2024, } @misc{CTT100920300, author = {Bo Zhu and Yukihiko Yamasaki and Takanobu Yasuda and Cheng Qian and Zhirou Qiu and Hiroshi Ueda and Tetsuya Kitaguchi}, title = {Customizable OpenGUS immunoassay: a homogeneous detection system using split β-glucuronidase and label-free antibody}, year = 2023, } @misc{CTT100908472, author = {安田 貴信 and 北口哲也}, title = {互いに引き合う螺旋状ペプチド : 二量体形成から細胞療法まで}, year = 2023, } @misc{CTT100908473, author = {安田 貴信 and 大森 健太 and 上田宏 and 北口哲也}, title = {トラップ&リリースが拓いた免疫センサーの道と抗体薬物複合体の未来}, year = 2023, } @misc{CTT100875117, author = {Akihito Inoue and Takanobu Yasuda and Bo Zhu and Tetsuya Kitaguchi and Akikazu Murakami and Hiroshi Ueda.}, title = {Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface.}, year = 2021, } @misc{CTT100907136, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, year = 2022, } @misc{CTT100866472, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, year = 2022, } @misc{CTT100866471, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, year = 2022, } @misc{CTT100866470, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, year = 2022, } @misc{CTT100869590, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {公開特許}, year = 2020, month = {}, note = {PCT/JP2020/021899(2020/06/03), WO 2020/246495(2020/10/12)} } @misc{CTT100917020, author = {上田宏 and 北口哲也 and 大室有紀 and 安田貴信 and 井上暁人}, title = {複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法}, howpublished = {登録特許}, year = 2024, month = {}, note = {特願2021-524873(2020/06/03), WO 2020/246495(2020/10/12), 特許第7461661号(2024/03/27)} } @phdthesis{CTT100907136, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, school = {東京工業大学}, year = 2022, } @phdthesis{CTT100866472, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, school = {東京工業大学}, year = 2022, } @phdthesis{CTT100866471, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, school = {東京工業大学}, year = 2022, } @phdthesis{CTT100866470, author = {安田貴信}, title = {高性能な蛍光・発光免疫センサー創出のための分子設計とその解析}, school = {東京工業大学}, year = 2022, }