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Title
Japanese: 
English:Direct Observation of Multiple and Stochastic Transition States by a Feedback-controlled Single-molecule Force Measurement 
Author
Japanese: 深川 暁宏, Michio HIROSHIMA, Isao SAKANE, 徳永 万喜洋.  
English: Akihiro FUKAGAWA, Michio HIROSHIMA, Isao SAKANE, Makio TOKUNAGA.  
Language English 
Journal/Book name
Japanese: 
English:ANALYTICAL SCIENCES 
Volume, Number, Page Vol. 25    No. 1    pp. 5-7
Published date Jan. 2009 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.2116/analsci.25.5
Abstract To overcome the ensemble-averaging barrier, single-molecule experiments have been performed, but energy landscapes comprising multiple intermediates have not yet been defined. We performed mechanical unfolding of staphylococcal nuclease using intermoleculer force microscopy, modified AFM with high resolution and feedback control of the positioning. The force dropped vertically just after its peak, and multiple transition states were detected as force peaks. The multiple and stochastic intermediates found in the present study provide new important information on protein folding.

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