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Title
Japanese: 
English:Newly generated T cell receptor microclusters initiate and sustain T cell activaion by recruitment of Zap70 and SLP-76 
Author
Japanese: Tadashi Yokosuka, 十川 久美子, Wakana Kobayashi, Michio Hiroshima, Akiko Hashimoto-Tane, 徳永 万喜洋, Michael L Dustin, Takashi Saito.  
English: Tadashi Yokosuka, Kumiko Sakata-Sogawa, Wakana Kobayashi, Michio Hiroshima, Akiko Hashimoto-Tane, Makio Tokunaga, Michael L Dustin, Takashi Saito.  
Language English 
Journal/Book name
Japanese: 
English:nature immunology 
Volume, Number, Page volume 6    number 12    pp. 1253-1262
Published date Dec. 2005 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
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DOI https://doi.org/10.1038/ni1272
Abstract T cell receptor (TCR) activation and signaling precede immunological synapse formation and are sustained for hours after initiation. However, the precise physical sites of the initial and sustained TCR signaling are not definitively known. We report here that T cell activation was initiated and sustained in TCR-containing microclusters generated at the initial contact sites and the periphery of the mature immunological synapse. Microclusters containing TCRs, the tyrosine kinase Zap70 and the adaptor molecule SLP-76 were continuously generated at the periphery. TCR microclusters migrated toward the central supramolecular cluster, whereas Zap70 and SLP-76 dissociated from these microclusters before the microclusters coalesced with the TCR-rich central supramolocular cluster. Tyrosine phosphorylation and calcium influx were induced as microclusters formed at the initial contact sites. Inhibition of signaling prevented recruitment of Zap70 into the microclusters. These results indicated that TCR-rich microclusters initiate and sustain TCR signaling.

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