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Title
Japanese: 
English:On-chip Single Embryo Coculture with Microporous Membrane-supported Endometrial Cells 
Author
Japanese: 山本貴富喜.  
English: Takatoki Yamamoto.  
Language English 
Journal/Book name
Japanese: 
English:IEEE Transactions on NanoBioscience 
Volume, Number, Page        
Published date Feb. 2010 
Publisher
Japanese: 
English:IEEE 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://ieeexplore.ieee.org/xpl/tocresult.jsp?isYear=2009&isnumber=5185450&Submit32=View+Contents
 
DOI https://doi.org/10.1109/TNB.2009.2035275
Abstract In vitro culture (IVC) of the mammalian embryo is an essential technique in reproductive technology and other related life science disciplines. Although embryos are usually cultured in groups, a single embryo culture has been highly desired for IVC to investigate developmental processes. In this study, we proposed and developed the first single embryo coculture device, which allows making an array of a single embryo coculture with endometrial cells by controlling the culture environment in a microfluidic device. To realize this concept, we investigated three key issues: selection of a culture medium for the embryo coculture with endometrial cells using a mouse embryo and endometrial cells; evaluation of an on-microporous-membrane coculture of endometrial cells and an embryo to control the polarization of endometrial cells on the membrane; and evaluation of the coculture of endometrial cells and the embryo in the microfluidic device. We successfully obtained an array of a single coculture of embryo with endometrial cells in a microfluidic device. This concept will open and enhance the management of an individual embryo for assisted reproductive technology, livestock breeding, and fundamental stage research by further development.

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