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Title
Japanese:免疫分子動態の可視化システム 
English:Molecular imaging and analysis of microclusters responsible for initiating T cell receptor signaling 
Author
Japanese: 十川久美子, 徳永万喜洋.  
English: Kumiko Sakata-Sogawa, makio tokunaga.  
Language Japanese 
Journal/Book name
Japanese:日本臨床 
English: 
Volume, Number, Page Vol. 65    No. 2    pp. 242-246
Published date Feb. 2007 
Publisher
Japanese: 
English: 
Conference name
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Conference site
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Abstract T cell receptors(TCR) are activated by a specific antigen and interact with other signaling molecules, such as kinases and adaptors. Aiming at analyzing precisely the dynamic process of T cell signaling, we used a combined system of a planar bilayer and TIRF microscopy. This system allowed us to observe the T cell activation process from the initial cell-bilayer contact(time 0). Our observation revealed that microclusters with TCR were generated at the initial contact to gather into central supramolecular cluster, the immunological synapse, which was believed to be responsible for T cell receptor signaling. Furthermore the microclusters were generated continuously at the periphery even at the sustained state and they migrated toward the central cluster. These results suggested the important role of microclusters in T cell activation.

©2007 Tokyo Institute of Technology All rights reserved.