Home >

news Help

Publication Information


Title
Japanese: 
English:Location of the ATPase site of myosin determined by three-dimensional electron microscopy 
Author
Japanese: 徳永 万喜洋, Kazuo Sutoh, Chikashi Toyoshima, Takeyuki Wakabayashi.  
English: Makio Tokunaga, Kazuo Sutoh, Chikashi Toyoshima, Takeyuki Wakabayashi.  
Language English 
Journal/Book name
Japanese: 
English:Nature 
Volume, Number, Page Vol. 329    No. 6140    pp. 635-638
Published date Oct. 1987 
Publisher
Japanese: 
English:Macmillan Journals Ltd 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Abstract Both ATP hydrolysis by myosin and the accompanying cyclic association-dissociation of actin and myosin are essential for muscle contraction. It is important for understanding the molecular mechanism of contraction to know the three-dimensional locations of the two major funcional sites of myosin:the ATPase site and the actin-binding site. We have determined the position of the ATPase site of myosin using three-dimensional image reconstruction from electron micrographs and site-specific labelling with the avidin-biotin system. The ATPase site is about 5nm from the tip of the myosin head and is about 4 nm away from the actin-binding site of myosin. This is the first report of the three-dimensional location of an enzyme active site by electron microscopy.

©2007 Tokyo Institute of Technology All rights reserved.