Attention location could be different from fixation point, although they are closely related. To predict attention state of human is important for development of human information systems and for understanding of attention mechanism. Although several techniques have been proposed to predict attention locations recently, none of them seems reliable enough for practical use. In our previous research, we found that there is a relationship between the luminance at attentional location and the size of pupil. Then, we investigated in this research the effect of attention shifts produced by exogenous and endogenous cues on the pupil size using the visual pattern having several areas with different luminance. Results indicated that pupil expanded when the luminance at attention location is low. In addition attention shifts by exogenous cue seemed to have a larger effect on pupil responses.