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Title
Japanese: 
English:Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K 
Author
Japanese: 内山 大輔, 大友 康平, 小井川 浩之, 南後 守, 出羽 毅久, 藤芳 暁, 松下 道雄.  
English: Daisuke Uchiyama, Kohei Otomo, Hiroyuki Oikawa, Mamoru Nango, Takehisa Dewa, SATORU FUJIYOSHI, Michio Matsushita.  
Language English 
Journal/Book name
Japanese: 
English:Physical Chemistry and Chemical Physics 
Volume, Number, Page Vol. 13        p. 11615-11619
Published date June 28, 2011 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
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Conference site
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DOI https://doi.org/10.1039/c1cp20172g
Abstract As a model of photosynthetic unit (PSU), self-assembled aggregates of pigment-protein complexes from photosynthetic bacteria were prepared in a lipid bilayer by reconstitution of the light-harvesting 2 (LH2) complex and light-harvesting 1-reaction center (LH1-RC) complex through detergent removal of their micelles in the presence of lipids. By performing polarization-controlled fluorescence and fluorescence-excitation spectroscopy on single aggregates at a temperature of 5 K, the composition of individual aggregates was determined and excitation energy transfer (EET) between constituent complexes was observed. LH2 and LH1-RC from a bacterium, Rhodobacter (Rb.) sphaeroides, were found to form a trimeric aggregate in which EET takes place from one LH2 to two LH1-RCs. In contrast, a heterodimer of LH2 and LH1-RC in which EET works was found to assemble from a combination of complexes of different bacterial species, that is, LH2 from Rb. sphaeroides and LH1-RC from Rhodopseudomonas (Rps.) palustris.

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