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Title
Japanese: 
English:Three-dimensional laser-scanning confocal reflecting microscope for multicolor single-molecule imaging at 1.5 K 
Author
Japanese: 丸尾美奈子, 稲川博敬, 虎谷泰靖, 近藤 徹, 松下道雄, 藤芳 暁.  
English: Minako Maruo, Hironori Inagawa, Yasuharu Toratani, Toru Kondo, Michio Matsushita, SATORU FUJIYOSHI.  
Language English 
Journal/Book name
Japanese: 
English:Chemical Physics Letters 
Volume, Number, Page Vol. 591        p. 233-236
Published date Jan. 20, 2014 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1016/j.cplett.2013.11.039
Abstract A three-dimensional (3D) laser-scanning confocal microscope was developed for multicolor 3D co-localization of single fluorescent molecules immobilized at a temperature of 1.5 K. The image quality was tested by taking 3D emission image of single quantum dots with three different excitation wavelengths of 635, 532 and 405 nm. The 3D image size was all within 1.2 times of the diffraction-limited size. From the centroid position of the 3D image taken with 635- and 532-nm excitations, 3D co-localization precision of single dot was determined to be 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.

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