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Title
Japanese:Design of Photoaffinity Probe Molecules for Identification and Modification of Target Proteins 
English:Design of Photoaffinity Probe Molecules for Identification and Modification of Target Proteins 
Author
Japanese: Hiroyuki Nakamura, Hyun Seung Ban, Kazuki Shimizu, Hidemitsu Minegishi, Shinichi Sato.  
English: Hiroyuki Nakamura, Hyun Seung Ban, Kazuki Shimizu, Hidemitsu Minegishi, Shinichi Sato.  
Language English 
Journal/Book name
Japanese:Journal of Photopolymer Science and Technology 
English:Journal of Photopolymer Science and Technology 
Volume, Number, Page Vol. 27        pp. 453-458
Published date Sept. 2014 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.2494/photopolymer.27.453
Abstract Techniques for the visualization of target proteins by small-molecule probes in living systems are highly important to investigate the function, dynamics, localization, and crosstalk of individual proteins. We have studied inhibitors of hypoxia-inducible factors (HIF), which are heterodimeric (α/β) transcriptional factors and major physiological stimuli for expression of angiogenesis factors. Inhibition of tumor-induced angiogenesis prevents growth of many types of solid tumors, thus is considered to provide a novel approach for cancer treatment. We recently developed carborane-containing phenoxyacetanilides as potent inhibitors of HIF-1α activation under hypoxia. We designed their photoaffinity labeling molecules to clarify the action mechanism against the HIF inhibition. Using the photoaffinity labeling molecules, we identified that heat shock protein (HSP) 60 is the target protein and clarified that HSP60 has an important role for stabilization of HIF-1α under hypoxia.

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