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Title
Japanese: 
English:Cooperative enhancement of deoxyribozymeactivity by chemical modification and addedcationic copolymer 
Author
Japanese: 齋藤 健, 嶋田 直彦, 丸山厚.  
English: Ken Saito, Naohiko Shimada, ATSUSHI MARUYAMA.  
Language English 
Journal/Book name
Japanese: 
English:Science and Technology of Advanced Materials 
Volume, Number, Page Vol. 17        p. 437-442
Published date June 29, 2016 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
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File
DOI http://dx.doi.org/10.1080/14686996.2016.1208627
Abstract Deoxyribozymes (DNAzymes) having RNA-cleaving activity have widely been explored as tools for therapeutic and diagnostic purposes. Both the chemical cleaving step and the turnover step should be improved for enhancing overall activity of DNAzymes. We have shown that cationic copolymer enhanced DNAzyme activity by increasing turnover efficacy. In this paper, effects of the copolymer on DNAzymes modified with locked nucleic acids (LNA) or 2′-O-methylated (2′-OMe) nucleic acids were studied. The copolymer increased activity of these chemically modified DNAzymes. More than 30-fold enhancement in multiple-turnover catalytic activity was observed with 2′-OMe-modified DNAzyme in the presence of the copolymer. DNAzyme catalytic activity was successfully enhanced by cooperation of the added copolymer and chemical modification of DNAzyme.

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