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Title
Japanese:Comparative subcellular localization analysis of magnetosome proteins reveals a unique localization behavior of Mms6 protein onto magnetite crystals 
English:Comparative subcellular localization analysis of magnetosome proteins reveals a unique localization behavior of Mms6 protein onto magnetite crystals 
Author
Japanese: Atsushi Arakaki, Daiki Kikuchi, Masayoshi Tanaka, Ayana Yamagishi, Takuto Yoda, Tadashi Matsunaga.  
English: Atsushi Arakaki, Daiki Kikuchi, Masayoshi Tanaka, Ayana Yamagishi, Takuto Yoda, Tadashi Matsunaga.  
Language English 
Journal/Book name
Japanese:Journal of Bacteriology 
English:Journal of Bacteriology 
Volume, Number, Page        
Published date Aug. 2016 
Publisher
Japanese:American Society for Microbiology 
English:American Society for Microbiology 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://jb.asm.org/lookup/doi/10.1128/JB.00280-16
 
DOI https://doi.org/10.1128/JB.00280-16
Abstract <p>The magnetosome is an organelle specialized for inorganic magnetite crystal synthesis in magnetotactic bacteria. The complex mechanism of magnetosome formation is regulated by magnetosome proteins in a stepwise manner. Protein localization is a key step for magnetosome development; however, a global study of magnetosome protein localization remains to be conducted. Here, we comparatively analyzed the subcellular localization of a series of GFP tagged magnetosome proteins. The protein localizations were categorized into 5 groups (short-length linear, middle-length linear, long-length linear, cell membrane, and intracellular dispersing), which were related to the protein functions. Mms6, which regulates magnetite crystal growth, localized along magnetosome chain structures under magnetite-forming (microaerobic) conditions, but was dispersed in the cell under non-forming (aerobic) conditions. Correlative fluorescence and electron microscopy analyses revealed that Mms6 preferentially localized to magnetosomes enclosing magnetite crystals. We suggest that a highly organized spatial-regulation mechanism controls magnetosome protein localization during magnetosome formation in magnetotactic bacteria.</p>

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