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Title
Japanese: 
English:Selective Purification and Chemical Labeling of Target Protein on Ruthenium Photocatalyst-Immobilized Affinity Beads 
Author
Japanese: M. Tsushima, 佐藤 伸一, 中村 浩之.  
English: M. Tsushima, S. Sato, H. Nakamura.  
Language English 
Journal/Book name
Japanese: 
English:Chem.Comm. 
Volume, Number, Page Vol. 53        Page 4838-4841
Published date Apr. 1, 2017 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1039/c7cc01595j
Abstract Selective purification and chemical labeling of a target protein in a protein mixture were simultaneously achieved on the surface of affinity beads functionalized with ligands, such as benzenesulfona- mide and methotrexate (MTX), and a ruthenium complex containing 2,20-bipyridine-4,40-dicarboxylic acid (dcbpy). Chemical labeling of the target protein with a tyrosine radical trapper (TRT) proceeded on the surface of the beads when the target protein was in close proximity to the ruthenium photocatalyst. Both the protein purifi- cation and chemical labeling abilities of the affinity beads functio- nalized with ruthenium photocatalyst were not compromised after recycling several times. Dihydrofolate reductase (DHFR) endogenously expressed in HeLa cells was detected by chemical labeling with biotin-TRT on the affinity beads with high sensitivity compared to the conventional silver staining method.

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