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Title
Japanese:Star-Shaped Thermoresponsive Polymers with Various Functional Groups for Cell Sheet Engineering 
English:Star-Shaped Thermoresponsive Polymers with Various Functional Groups for Cell Sheet Engineering 
Author
Japanese: Yu Sudo, Ryuki Kawai, Hideaki Sakai, Ryohei Kikuchi, Yuta Nabae, Teruaki Hayakawa, Masa-aki Kakimoto.  
English: Yu Sudo, Ryuki Kawai, Hideaki Sakai, Ryohei Kikuchi, Yuta Nabae, Teruaki Hayakawa, Masa-aki Kakimoto.  
Language English 
Journal/Book name
Japanese:Langmuir 
English:Langmuir 
Volume, Number, Page Vol. 34    No. 2    pp. 653-662
Published date Dec. 19, 2017 
Publisher
Japanese:American Chemical Society 
English:American Chemical Society 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://dx.doi.org/10.1021/acs.langmuir.7b04213
 
DOI https://doi.org/10.1021/acs.langmuir.7b04213
Abstract This study demonstrates the facile preparation of poly(N-isopropylacrylamide) (PNIPAM)-immobilized Petri dishes by drop-casting a star-shaped copolymer of hyperbranched polystyrene (HBPS) possessing PNIPAM arms (HBPS-g-PNIPAM) functionalized with polar groups. HBPS was synthesized via reversible addition窶吐ragmentation chain transfer (RAFT) self-condensing vinyl polymerization (SCVP), and HBPS polymers with different terminal structures were prepared by changing the monomer structure. HBPS-g-PNIPAM was synthesized by the grafting of PNIPAM from each terminal of HBPS. To tune the cell adhesion and detachment properties, polar functional groups such as carboxylic acid and dimethylamino groups were introduced to HBPS-g-PNIPAM. Based on surface characterization using scanning transmission electron microscopy (STEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements, the advantage of the hyperbranched structure for the PNIPAM immobilization was evident in terms of the uniformity, stability, and thermoresponsiveness. Successful cell sheet harvesting was demonstrated on dishes coated with HBPS-g-PNIPAM. In addition, the cell adhesion and detachment properties could be tuned by the introduction of polar functional groups.

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