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Title
Japanese:Rapid colorimetric antibody detection using a dual-function peptide probe for silver nanoparticle aggregation and antibody recognition. 
English:Rapid colorimetric antibody detection using a dual-function peptide probe for silver nanoparticle aggregation and antibody recognition. 
Author
Japanese: Mina Okochi, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda.  
English: Mina Okochi, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda.  
Language English 
Journal/Book name
Japanese:Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 
English:Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 
Volume, Number, Page Vol. 32    No. 1    pp. 93-7
Published date Jan. 2016 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://europepmc.org/abstract/med/26753712
 
DOI https://doi.org/10.2116/analsci.32.93
Abstract Simple and rapid tools for antibody detection are beneficial for therapeutic monoclonal antibody development. Using a synthetic cationic antibody-recognizing peptide, a label-free colorimetric assay for antibody detection was established, in which a solution containing silver nanoparticles (AgNPs) changes color depending on particle aggregation/dispersion. Among the peptide probes we previously screened as IgG binding, one (NKFRGKYK) has four cationic amino acids and a pI of 10.46. Hence, we hypothesized that the peptide would both bind IgG and induce anionic AgNP aggregation via electrostatic interactions. This dual functionality of the IgG binding peptide could be useful for colorimetric detection. The detection of IgG in a solution containing culture media was investigated, and IgG was successfully detected at 100 to 500 nM within 2 min. This method is promising for high-throughput selection of IgG-producing cells since it is fast, readily observable, and does not involve complicated nanoparticle functionalization.

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