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Title
Japanese: 
English:A simple, real-time assay of horseradish peroxidase using biolayer interferometry. 
Author
Japanese: Takaaki Kojima, Ayako Nakane, 朱 博, Almasul Alfi, Hideo Nakano.  
English: Takaaki Kojima, Ayako Nakane, Bo Zhu, Almasul Alfi, Hideo Nakano.  
Language English 
Journal/Book name
Japanese:Bioscience, biotechnology, and biochemistry 
English:Bioscience, biotechnology, and biochemistry 
Volume, Number, Page Vol. 83    No. 10    pp. 1822-1828
Published date May 23, 2019 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1080/09168451.2019.1621156
 
DOI https://doi.org/10.1080/09168451.2019.1621156
Abstract Horseradish peroxidase (HRP) isoenzyme C1a is one of the most widely used enzymes for various analytical methods in bioscience research and medical fields. In these fields, real-time monitoring of HRP activity is highly desirable because the utility of HRP as a reporter enzyme would be expanded. In this study, we developed a simple assay system enabling real-time monitoring of HRP activity by using biolayer interferometry (BLI). The HRP activity was quantitatively detected on a BLI sensor chip by tracing a binding response of tyramide, a substrate of HRP, onto an immobilized protein. This system could be applied to analyses related to oxidase activity, as well as to the functional analysis of recombinant HRP.

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