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Title
Japanese: 
English:Construction of a DNA Library on Microbeads Using Whole Genome Amplification. 
Author
Japanese: KOJIMA Takaaki, 朱 博, NAKANO Hideo.  
English: KOJIMA Takaaki, ZHU Bo, NAKANO Hideo.  
Language English 
Journal/Book name
Japanese: 
English:In: Kroneis T. (eds) Whole Genome Amplification, Methods in Molecular Biology 
Volume, Number, Page        
Published date Jan. 1, 2015 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1007/978-1-4939-2990-0_6
 
DOI https://doi.org/10.1007/978-1-4939-2990-0_6
Abstract A polymerase chain reaction (PCR) in water droplets with water-in-oil emulsion (emulsion PCR) facilitates parallel amplification of a single-molecule template. The amplified DNA can be immobilized onto microbeads bound to primer DNA. The product, termed a "bead library", has various applications such as next-generation sequencing (NGS) and the directed evolution of various functional biomolecules. Here, we describe a method for genomic library construction on microbeads using emulsion PCR.

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