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Title
Japanese:Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins. 
English:Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins. 
Author
Japanese: 對馬理彦, 佐藤伸一, 丹羽達也, 田口英樹, 中村浩之.  
English: Michihiko Tsushima, Shinichi Sato, Tatsuya Niwa, HIDEKI TAGUCHI, Hiroyuki Nakamura.  
Language English 
Journal/Book name
Japanese:Chemical communications (Cambridge, England) 
English:Chemical communications (Cambridge, England) 
Volume, Number, Page Vol. 55    No. 88    Page 13275-13278
Published date Oct. 2019 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1039/c9cc05231c
 
DOI https://doi.org/10.1039/c9cc05231c
Abstract Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.

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