Home >

news Help

Publication Information


Title
Japanese: 
English:Molecular characterization of the role of the Schizosaccharomyces pombe nip1<sup>+</sup>/ctp1<sup>+</sup>gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex 
Author
Japanese: Akamatsu, Y., 村山泰斗, Yamada, T., Nakazaki, T., 筒井康博, Ohta, K., 岩崎博史.  
English: Akamatsu, Y., Yasuto Murayama, Yamada, T., Nakazaki, T., Yasuhiro Tsutsui, Ohta, K., Hiroshi Iwasaki.  
Language English 
Journal/Book name
Japanese:Molecular and Cellular Biology 
English:Molecular and Cellular Biology 
Volume, Number, Page Vol. 28    No. 11    pp. 3639-3651
Published date June 2008 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-44349086462&partnerID=MN8TOARS
 
DOI https://doi.org/10.1128/MCB.01828-07
Abstract The Schizosaccharomyces pombe nip1+/ctp1+ gene was previously identified as an slr (synthetically lethal with rad2) mutant. Epistasis analysis indicated that Nip1/Ctp1 functions in Rhp51-dependent recombinational repair, together with the Rad32 (spMre11)-Rad50-Nbs1 complex, which plays important roles in the early steps of DNA double-strand break repair. Nip1/Ctp1 was phosphorylated in asynchronous, exponentially growing cells and further phosphorylated in response to bleomycin treatment. Overproduction of Nip1/Ctp1 suppressed the DNA repair defect of an nbs1-s10 mutant, which carries a mutation in the FHA phosphopeptide-binding domain of Nbs1, but not of an nbs1 null mutant. Meiotic DNA double-strand breaks accumulated in the nip1/ctp1 mutant. The DNA repair phenotypes and epistasis relationships of nip1/ctp1 are very similar to those of the Saccharomyces cerevisiae sae2/com1 mutant, suggesting that Nip1/Ctp1 is a functional homologue of Sae2/Com1, although the sequence similarity between the proteins is limited to the C-terminal region containing the RHR motif. We found that the RxxL and CxxC motifs are conserved in Schizosaccharomyces species and in vertebrate CtIP, originally identified as a cofactor of the transcriptional corepressor CtBP. However, these two motifs are not found in other fungi, including Saccharomyces and Aspergillus species. We propose that Nip1/Ctp1 is a functional counterpart of Sae2/Com1 and CtIP.

©2007 Tokyo Institute of Technology All rights reserved.