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Title
Japanese: 
English:The Swi5-Sfr1 complex stimulates Rhp51/Rad51 - and Dmc1-mediated DNA strand exchange in vitro 
Author
Japanese: 春田奈美, 黒川裕美子, 村山泰斗, 赤松由布子, Unzai, S., Tsutsui, Y., 岩崎博史.  
English: Haruta, N., Yumiko Kurokawa, Yasuto Murayama, Akamatsu, Y., Unzai, S., Tsutsui, Y., Hiroshi Iwasaki.  
Language English 
Journal/Book name
Japanese:Nature Structural and Molecular Biology 
English:Nature Structural and Molecular Biology 
Volume, Number, Page Vol. 13    No. 9    pp. 823-830
Published date Sept. 2006 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-33748357743&partnerID=MN8TOARS
 
DOI https://doi.org/10.1038/nsmb1136
Abstract Nucleoprotein filaments made up of Rad51 or Dmc1 recombinases, the core structures of recombination, engage in ATP-dependent DNA-strand exchange. The ability of recombinases to form filaments is enhanced by recombination factors termed 'mediators'. Here, we show that the Schizosaccharomyces pombe Swi5-Sfr1 complex, a conserved eukaryotic protein complex, at substoichiometric concentrations stimulates strand exchange mediated by Rhp51 (the S. pombe Rad51 homolog) and Dmc1 on long DNA substrates. Reactions mediated by both recombinases are completely dependent on Swi5-Sfr1, replication protein A (RPA) and ATP, although RPA inhibits the reaction when it is incubated with single-stranded DNA (ssDNA) before the recombinase. The Swi5-Sfr1 complex overcomes, at least partly, the inhibitory effect of RPA, representing a novel class of mediator. Notably, the Swi5-Sfr1 complex preferentially stimulates the ssDNA-dependent ATPase activity of Rhp51, and it increases the amounts of Dmc1 bound to ssDNA.

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