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Title
Japanese: 
English:Atomic structure of the RuvC resolvase: A holliday junction-specific endonuclease from E. coli 
Author
Japanese: Ariyoshi, M., Vassylyev, D.G., 岩崎博史, Nakamura, H., Shinagawa, H., Morikawa, K..  
English: Ariyoshi, M., Vassylyev, D.G., Hiroshi Iwasaki, Nakamura, H., Shinagawa, H., Morikawa, K..  
Language English 
Journal/Book name
Japanese:Cell 
English:Cell 
Volume, Number, Page Vol. 78    No. 6    pp. 1063-1072
Published date Sept. 1994 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-0028120546&partnerID=MN8TOARS
 
DOI https://doi.org/10.1016/0092-8674(94)90280-1
Abstract The crystal structure of the RuvC protein, a Holliday junction resolvase from E. coli, has been determined at 2.5 A resolution. The enzyme forms a dimer of 19 kDa subunits related by a dyad axis. Together with results from extensive mutational analyses, the refined structure reveals that the catalytic center, comprising four acidic residues, lies at the bottom of a cleft that nicely fits a DNA duplex. The structural features of the dimer, with a 30 A spacing between the two catalytic centers, provide a substantially defined image of the Holliday junction architecture. The folding topology in the vicinity of the catalytic site exhibits a striking similarity to that of RNAase H1 from E. coli.

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