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Title
Japanese: 
English:ATP-dependent resolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase 
Author
Japanese: Fukuoh, A., 岩崎博史, Ishioka, K., Shinagawa, H..  
English: Fukuoh, A., Hiroshi Iwasaki, Ishioka, K., Shinagawa, H..  
Language English 
Journal/Book name
Japanese:EMBO Journal 
English:EMBO Journal 
Volume, Number, Page Vol. 16    No. 1    pp. 203-209
Published date Jan. 1997 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-0031028107&partnerID=MN8TOARS
 
DOI https://doi.org/10.1093/emboj/16.1.203
Abstract The RecG protein of Escherichia coli is a DNA helicase that promotes branch migration of the Holliday junctions. We found that overproduction of RecG protein drastically decreased copy numbers of ColE1-type plasmids, which require R-loop formation between the template DNA and a primer RNA transcript (RNA II) for the initiation of replication. RecG efficiently inhibited in vitro ColE1 DNA synthesis in a reconstituted system containing RNA polymerase, RNase HI and DNA polymerase I. RecG promoted dissociation of RNA II from the R-loop in a manner that required ATP hydrolysis. These results suggest that overproduced RecG inhibits the initiation of replication by prematurely resolving the R-loops formed at the replication origin region of these plasmids with its unique helicase activity. The possibility that RecG regulates the initiation of a unique mode of DNA replication, oriC-independent constitutive stable DNA replication, by its activity in resolving R-loops is discussed.

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