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Title
Japanese: 
English:SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: Functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA 
Author
Japanese: Shiba, T., 岩崎博史, Nakata, A., Shinagawa, H..  
English: Shiba, T., Hiroshi Iwasaki, Nakata, A., Shinagawa, H..  
Language English 
Journal/Book name
Japanese:Proceedings of the National Academy of Sciences of the United States of America 
English:Proceedings of the National Academy of Sciences of the United States of America 
Volume, Number, Page Vol. 88    No. 19    pp. 8445-8449
Published date Oct. 1991 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
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Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-0026058539&partnerID=MN8TOARS
 
DOI https://doi.org/10.1073/pnas.88.19.8445
Abstract The ruv operon is induced by treatments that damage DNA and is regulated by the LexA repressor. It encodes two proteins, RuvA and RuvB, that are involved in DNA repair, recombination in RecE and RecF pathways, and mutagenesis. RuvB protein was previously purified and has ATP-binding activity and weak ATPase activity. To study the biochemical properties of RuvA and its interaction with RuvB, we purified RuvA protein to near homogeneity from an over-producing strain. RuvA bound more efficiently to single-stranded DNA than to double-stranded DNA. RuvA bound to DNA greatly enhanced the ATPase activity of RuvB; the enhancing effect of various forms of DNA was in the order of supercoiled DNA greater than single-stranded DNA greater than linear double-stranded DNA. UV irradiation further enhanced the ATPase stimulatory effect of supercoiled DNA dose dependently. The RuvA-RuvB complex has an activity that renatures the cruciform structure in supercoiled DNA. From these experiments and previous work, we infer that the RuvA-RuvB complex may promote branch migration in recombination and may correct irregular structures in DNA, such as cruciforms and hairpins, to facilitate DNA repair using ATP as the energy source.

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