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Title
Japanese: 
English:Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase a: Evidence for a multifunctional role of Mcl1 in DNA replication and repair 
Author
Japanese: 筒井康博, Morishita, T., Natsume, T., Yamashita, K., 岩崎博史, Yamao, F., Shinagawa, H..  
English: Yasuhiro Tsutsui, Morishita, T., Natsume, T., Yamashita, K., Hiroshi Iwasaki, Yamao, F., Shinagawa, H..  
Language English 
Journal/Book name
Japanese:Current Genetics 
English:Current Genetics 
Volume, Number, Page Vol. 48    No. 1    pp. 34-43
Published date July 2005 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
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Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-23144459109&partnerID=MN8TOARS
 
DOI https://doi.org/10.1007/s00294-005-0584-2
Abstract Schizosaccharomyces pombe rad2 is involved in Okazaki fragments processing during lagging-strand DNA replication. Previous studies identified several slr mutants that are co-lethal with rad2Delta and sensitive to methyl methanesulfonate as single mutants. One of these mutants, slr3-1, is characterized here. Complementation and sequence analyses show that slr3-1 (mcl1-101) is allelic to mcl1(+), which is required for chromosome replication, cohesion and segregation. mcl1-101 is temperature-sensitive for growth and is highly sensitive to DNA damage. mcl1 cells arrest with 2C DNA content and chromosomal DNA double-strand breaks accumulate at the restrictive temperature. Mcl1p, which belongs to the Ctf4p/SepBp family, interacts both genetically and physically with DNA polymerase alpha. Mutations in rhp51 and dna2 enhance the growth defect of the mcl1-101 mutant. These results strongly suggest that Mcl1p is a functional homologue of Saccharomyces cerevisiae Ctf4p and plays a role in lagging-strand synthesis and Okazaki fragment processing, in addition to DNA repair.

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