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Title
Japanese: 
English:Crystal structure of the RuvA-RuvB complex: A structural basis for the holliday junction migrating motor machinery 
Author
Japanese: Yamada, K., Miyata, T., Tsuchiya, D., Oyama, T., Fujiwara, Y., Ohnishi, T., S. Shinagawa, Ariyoshi, M., Mayanagi, K., Morikawa, K., 岩崎博史.  
English: Yamada, K., Miyata, T., Tsuchiya, D., Oyama, T., Fujiwara, Y., Ohnishi, T., S. Shinagawa, Ariyoshi, M., Mayanagi, K., Morikawa, K., Hiroshi Iwasaki.  
Language English 
Journal/Book name
Japanese:Molecular Cell 
English:Molecular Cell 
Volume, Number, Page Vol. 10    No. 3    pp. 671-681
Published date Sept. 2002 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-18644379392&partnerID=MN8TOARS
 
DOI https://doi.org/10.1016/S1097-2765(02)00641-X
Abstract We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.

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