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Title
Japanese: 
English:Structural analyses of synthetic immobile Holliday junction. 
Author
Japanese: Shida, T., 岩崎博史, Shinagawa, H., Kyogoku, Y..  
English: Shida, T., Hiroshi Iwasaki, Shinagawa, H., Kyogoku, Y..  
Language English 
Journal/Book name
Japanese:Nucleic acids symposium series 
English:Nucleic acids symposium series 
Volume, Number, Page     No. 29    pp. 181-182
Published date 1993 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://www.scopus.com/inward/record.url?eid=2-s2.0-0027430588&partnerID=MN8TOARS
 
Abstract In order to study the structural properties of Holliday junctions, we designed and synthesized oligodeoxyribonucleotides to construct the models of the Holliday junctions with no sequence symmetry at the sites of branching called immobile junctions. Electrophoretic and spectroscopic analyses of the immobile junctions indicated that the Holliday junctions constructed from four deoxy 24-mers and four 18-mers were stable in 0.1 M NaCl at approximately 5 x 10(-6) M strand concentration at room temperature. Addition of MgCl2 to the solution of HJs resulted significantly in an increase in melting temperature of HJs.

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