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Title
Japanese:A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors. 
English:A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors 
Author
Japanese: Afshar Negar, Argunhan Bilge, ﾊﾟﾙﾊｯﾄ ﾏﾙﾀﾞﾝ, 谷口 剛樹, 坪内 英生, 岩崎 博史.  
English: Negar Afshar, Bilge Argunhan, Maierdan Palihati, Goki Taniguchi, Hideo Tsubouchi, Hiroshi Iwasaki.  
Language English 
Journal/Book name
Japanese:eLife 
English:eLife 
Volume, Number, Page Vol. 10        e64131
Published date Jan. 25, 2021 
Publisher
Japanese: 
English:eLife Sciences Publications 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
File
Official URL http://europepmc.org/abstract/med/33493431
 
DOI https://doi.org/10.7554/elife.64131
Abstract Homologous recombination (HR) is essential for maintaining genome stability. Although Rad51 is the key protein that drives HR, multiple auxiliary factors interact with Rad51 to potentiate its activity. Here, we present an interdisciplinary characterization of the interactions between Rad51 and these factors. Through structural analysis, we identified an evolutionarily conserved acidic patch of Rad51. The neutralization of this patch completely abolished recombinational DNA repair due to defects in the recruitment of Rad51 to DNA damage sites. This acidic patch was found to be important for the interaction with Rad55-Rad57 and essential for the interaction with Rad52. Furthermore, biochemical reconstitutions demonstrated that neutralization of this acidic patch also impaired the interaction with Rad54, indicating that a single motif is important for the interaction with multiple auxiliary factors. We propose that this patch is a fundamental motif that facilitates interactions with auxiliary factors and is therefore essential for recombinational DNA repair.

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