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Title
Japanese:Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant 
English:Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant 
Author
Japanese: 蒲池利章, 伊藤栄紘, 吉森孝成, 堀内浩太.  
English: Toshiaki Kamachi, Hidehiro Ito, Kosei Yoshimori, Kohta Horiuchi.  
Language English 
Journal/Book name
Japanese:Frontiers in Microbiology 
English:Frontiers in Microbiology 
Volume, Number, Page Vol. 12       
Published date Mar. 22, 2021 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL http://dx.doi.org/10.3389/fmicb.2021.639266
 
DOI https://doi.org/10.3389/fmicb.2021.639266
Abstract <jats:p>Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol production system wherein MDH expression is controlled by using methanotroph mutants. We used the MxaF knockout mutant of <jats:italic>Methylosinus trichosporium</jats:italic> OB3b. It could only grow with MDH (XoxF) which has a cerium ion in its active site and is only expressed by bacteria in media containing cerium ions. In the presence of 0 μM copper ion and 25 μM cerium ion, the mutant grew normally. Under conditions conducive to methanol production (10 μM copper ion and 0 μM cerium ion), cell growth was inhibited and methanol accumulated (2.6 μmol·mg<jats:sup>−1</jats:sup> dry cell weight·h<jats:sup>−1</jats:sup>). The conversion efficiency of the accumulated methanol to the total amount of methane added to the reaction system was ~0.3%. The aforementioned conditions were repeatedly alternated by modulating the metal ion composition of the bacterial growth medium.</jats:p>

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