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Title
Japanese: 
English:Mapping the diffusion pattern of 1O2 along DNA duplex by guanine photooxidation with an appended biphenyl photosensitizer 
Author
Japanese: 金森功吏, 金子翔大, 浜本航治, 湯浅英哉.  
English: Takashi Kanamori, Shota Kaneko, Koji Hamamoto, HIDEYA YUASA.  
Language English 
Journal/Book name
Japanese: 
English:Scientific Reports 
Volume, Number, Page Vol. 13        288
Published date Jan. 23, 2023 
Publisher
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English: 
Conference name
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Conference site
Japanese: 
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DOI https://doi.org/10.1038/s41598-023-27526-2
Abstract To realize nucleic acid-targeting photodynamic therapy, a photosensitizer should be attached at the optimal position on a complementary oligonucleotide, where a guanine photooxidation is maximized. Here we show the photooxidation of 22 DNA duplexes with varied lengths between a 1O2-generating biphenyl photosensitizer attached at a midchain thymine in a strand and the single guanine reactant in the other strand. The best photooxidation efficiencies are achieved at 9, 10, and 21 base intervals, which coincides with the pitch of 10.5 base pairs per turn in a DNA duplex. The low efficiencies for near and far guanines are due to quenching of the biphenyl by guanine and dilution of 1O2 by diffusion, respectively. The 1O2-diffusion mapping along DNA duplex provides clues to the development of efficient and selective photosensitizer agents for nucleic acid-targeting photodynamic therapy, as well as an experimental demonstration of diffusion of a particle along cylindrical surface in molecular level.

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