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Title
Japanese:Intracellular photocatalytic-proximity labeling for profiling protein-protein interactions in microenvironments. 
English:Intracellular photocatalytic-proximity labeling for profiling protein-protein interactions in microenvironments. 
Author
Japanese: Tsushima M, Shinichi Sato, Kazuki Miura, 丹羽達也, Taguchi H, Hiroyuki Nakamura.  
English: Tsushima M, Shinichi Sato, Kazuki Miura, Tatsuya Niwa, Taguchi H, Hiroyuki Nakamura.  
Language English 
Journal/Book name
Japanese:Chemical communications (Cambridge, England) 
English:Chemical communications (Cambridge, England) 
Volume, Number, Page        
Published date Feb. 8, 2022 
Publisher
Japanese: 
English: 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
Official URL https://doi.org/10.1039/d1cc05764b
 
DOI https://doi.org/10.1039/d1cc05764b
Abstract Intracellular photocatalytic-proximity labeling (iPPL) was developed to profile protein-protein interactions in the microenvironment of living cells. Acriflavine was found to be an efficient cell-membrane-permeable photocatalyst for introduction into the genetically HaloTag-fused protein of interest for iPPL with a radical labeling reagent, 1-methyl-4-arylurazole. iPPL was applied to the histone-associated protein H2B in HaloTag-H2B expressing HEK293FT cells. The proteins directly interacting with histones and RNA-binding proteins were selectively labeled in the intracellular environment, suggesting that the iPPL method has a smaller labeling radius (CA. 6 nm) than the BioID and APEX methods.

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