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Title
Japanese:Dynamics of Single-Stranded RNA Looping Probed and Photoregulated by Sulfonated Pyrene 
English:Dynamics of Single-Stranded RNA Looping Probed and Photoregulated by Sulfonated Pyrene 
Author
Japanese: Jie Xu, Sachiko Tojo, Mamoru Fujitsuka, Kiyohiko Kawai.  
English: Jie Xu, Sachiko Tojo, Mamoru Fujitsuka, Kiyohiko Kawai.  
Language English 
Journal/Book name
Japanese:ChemistrySelect 
English:ChemistrySelect 
Volume, Number, Page Vol. 5    No. 26    pp. 8002-8008
Published date July 13, 2020 
Publisher
Japanese:Wiley-VCH Verlag GmbH & Co. KGaA 
English:Wiley-VCH Verlag GmbH & Co. KGaA 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1002/slct.202002231
Abstract RNAs especially the noncoding RNAs which lack protein-coding capacity but have critical functions in a wide range of cellular processes have received increased attention. They are reported to be associated with normal development and physiol., and their disruption would cause cancer. RNAs are dynamic mols. and the structural transition of them is fundamental to many RNA-involved cellular processes. The study of transition dynamics is critical to gaining knowledge on how the transition occurs, what factors govern the transition, and how to externally regulate the transition. Previously, we have reported the synthesis of sulfonated pyrene, SPy, and its application as a photoregulator for single-stranded DNA (ssDNA) looping. Herein, we studied the dynamics of single-stranded RNA (ssRNA) looping in aqueous solution by using SPy as both a probe and a photoregulator. The rate of end-to-end loop formation in ssRNA regulated by SPy upon photostimulation suggests a retarding impact of the ribose 2'-OH. The results also attest that (rU)n sequences are endowed with higher flexibility compared with (dT)n sequences and demonstrate the slowing down effect of C5-CH3 group in thymine base on the looping dynamics of polythymine sequences. The photoregulated ssRNA looping observed herein occurs at ﾎｼs to tens of ﾎｼs time scales. All the findings provide important insight into the flexibility and structural transition dynamics of oligonucleotides.

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