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Title
Japanese:Kinetics of Photoinduced Reactions at the Single-Molecule Level: The KACB Method 
English:Kinetics of Photoinduced Reactions at the Single-Molecule Level: The KACB Method 
Author
Japanese: Kiyohiko Kawai, Atsushi Maruyama.  
English: Kiyohiko Kawai, Atsushi Maruyama.  
Language English 
Journal/Book name
Japanese:Chemistry - A European Journal 
English:Chemistry - A European Journal 
Volume, Number, Page Vol. 26    No. 35    pp. 7740-7746
Published date Feb. 2020 
Publisher
Japanese:Wiley-VCH Verlag GmbH & Co. KGaA 
English:Wiley-VCH Verlag GmbH & Co. KGaA 
Conference name
Japanese: 
English: 
Conference site
Japanese: 
English: 
DOI https://doi.org/10.1002/chem.202000439
Abstract A review. The kinetics of photoinduced reactions can be approached by laser flash photolysis techniques. Although such techniques allow for a detailed understanding of the important photophysics of mols., they normally require a substantial amount of sample for measurements (>1 nmol), and thus, they are difficult to apply to anal. and diagnostic applications. The photophysics of a fluorescent mol. can be accessed by monitoring the kinetics of the fluctuation of fluorescence, which is called blinking. Blinking is a phenomenon that can be monitored only if mols. are observed at the single-mol. level. In bulk solution, blinking kinetics can be measured by using fluorescence correlation spectroscopy (FCS), which normally requires >105 times less sample than that required for laser flash photolysis. Blinking is controlled to extract fruitful microenvironmental information around a fluorescent mol., by using a method named kinetic anal. based on the control of fluorescence blinking (KACB). This Concept highlights the adaptation of the KACB method to study the local conformation of DNA with <1 pmol of DNA sample.

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