Site-directed antibodies were raised against three synthetic peptides whose sequences correspond to a region around the reactive llysine residue and two protease-sensitive regions of subfragment 1 (S1) of skeletal musle myosin (one at the junction of the 23,000 Mr and 50,000 Mr segments, the J1 junction; and the other at the junction of the 50,000 Mr and 20,000 Mr segments of the heavy chain, the J2 junction). The antisera cross-reacted with intact myosin with titres of 5×10 4 (anti-J1 antiserum) and 10 4 (anti-J2 and anti-reactive lysine residue antisera). Site-specific antibodies purified by S1-Sepharose readily bound to myosin. Electron microscopic examinations of antibody-myosin complexes revealed that the J1 and J2 junctions are located 15 nm and 16 nm from the head-rod junction, respectively, while the reactive lysine residue region is 13nm from the junction.
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