Position of the Amino Terminus of Myosin Light Chain 1 and Light Chain 2 Determined by Electron Microscopy with Monoclonal Antibody
著者
和文:
徳永 万喜洋,
Masashi Suzuki,
Kimiko Saeki,
Takeyuki Wakabayashi.
英文:
Makio Tokunaga,
Masashi Suzuki,
Kimiko Saeki,
Takeyuki Wakabayashi.
言語
English
掲載誌/書名
和文:
英文:
J. Mol. Biol.
巻, 号, ページ
Vol. 194
No. 2
pp. 245-255
出版年月
1987年5月
出版者
和文:
英文:
会議名称
和文:
英文:
開催地
和文:
英文:
アブストラクト
The position of the N terminus of myosin light chain 1 (LC1) and myosin light chain 2 (LC2) of rabbit skeletal muscle was mapped on the myosin head with a monoclonal antibody (SⅠ304), which recognizes the amino acid sequence N-trimethylalanyl-prolyl-lysyl-lysyl at the N terminus of LC1 and LC2. The complex of the antibody and myosin was observed by electron microscopy. By selective cleavage of the N terminus of LC1 or LC2 with papain or chymotrypsin, the position of the N terminus of LC1 and LC2 was determined separately. The N terminus of LC2 is located at the head-rod junction. The N terminus of LC1 is 11nm(±3nm, standard deviation) from the head-rod junction. This position is near the actin-binding site of the myosin head.
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