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タイトル
和文: 
英文:Structural analyses of synthetic immobile Holliday junction. 
著者
和文: Shida, T., 岩崎博史, Shinagawa, H., Kyogoku, Y..  
英文: Shida, T., Hiroshi Iwasaki, Shinagawa, H., Kyogoku, Y..  
言語 English 
掲載誌/書名
和文:Nucleic acids symposium series 
英文:Nucleic acids symposium series 
巻, 号, ページ     No. 29    pp. 181-182
出版年月 1993年 
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開催地
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公式リンク http://www.scopus.com/inward/record.url?eid=2-s2.0-0027430588&partnerID=MN8TOARS
 
アブストラクト In order to study the structural properties of Holliday junctions, we designed and synthesized oligodeoxyribonucleotides to construct the models of the Holliday junctions with no sequence symmetry at the sites of branching called immobile junctions. Electrophoretic and spectroscopic analyses of the immobile junctions indicated that the Holliday junctions constructed from four deoxy 24-mers and four 18-mers were stable in 0.1 M NaCl at approximately 5 x 10(-6) M strand concentration at room temperature. Addition of MgCl2 to the solution of HJs resulted significantly in an increase in melting temperature of HJs.

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