This study assessed the on-road gaseous and particulate emissions from a gasoline direct injection (GDI) hybrid electric vehicle (HEV) using portable emissions measurement systems (PEMS). Testing was conducted, while the vehicle was exercised over three routes with different topological and environmental characteristics, representing urban, highway, and mountain (high-altitude) driving conditions. The gaseous emissions (NOx, CO, THC, and CO2) were found to be the highest during mountain driving compared to urban and highway driving. PM mass and soot mass emissions were comparable between the Downtown LA (urban driving conditions) and Mt Baldy (rural driving conditions with altitude changes) routes but higher compared to Highway route. NOx emissions and soot mass emissions over all test routes exhibited reasonably good correlations with vehicle acceleration but poor correlations with road grade.