論文

• Sasuga, S., Abe, R., Nikaido, O., Kiyosaki, S., Sekiguchi, H., Ikai, A., TOSHIYA OSADA. Interaction between pheromone and its receptor of the fission yeast Schizosaccharomyces pombe examined by a force spectroscopy study, Journal of Biomedicine and Biotechnology, Vol. 2012, 2012.
• Hakari, T., Sekiguchi, H., TOSHIYA OSADA, Kishimoto, K., Afrin, R., Ikai, A.. Nonlinear displacement of ventral stress fibers under externally applied lateral force by an atomic force microscope, Cytoskeleton, Vol. 68, No. 11, pp. 628-638, 2011.
• Lesoil, C., Nonaka, T., Sekiguchi, H., TOSHIYA OSADA, Miyata, M., Afrin, R., Ikai, A.. Molecular shape and binding force of Mycoplasma mobile's leg protein Gli349 revealed by an AFM study, Biochemical and Biophysical Research Communications, Vol. 391, No. 3, pp. 1312-1317, 2010.
• Mahichi Faezeh, Aidan Synnott, Keiko Yamamichi, TOSHIYA OSADA, YASUNORI TANJI. Recombination of T2 bacteriophage using IP008 phage long tail fiber genes expands host range while retaining lytic activity, FEMS Microbiol. Letter, Vol. 295, pp. 211-217, 2009.
• 1. Kim H, Arakawa H, Hatae N, Sugimoto Y, Matsumoto O, Osada T, Ichikawa A, Ikai A. Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM, Ultramicroscopy, Vol. 106, pp. 652-662, 2006.
• Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M.. Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons., Chem Senses, Vol. 30, pp. 111-119, 2005.
• Kim, H., Satoshi Tsuruta, Hideo Arakawa, Osada, T., Ikai, A. Quantitative analysis of the number of antigens immobilized on a glass surface by AFM., Ultramicroscopy, Vol. 100, pp. 203-210, 2004.
• Uehara H. Osada, T., Ikai, A. Quantitative measurement of mRNA at different loci within an individual living cell, Ultramicroscopy, Vol. 100, pp. 197-201, 2004.
• Takigami S, Wakabayashi Y, Ohsako S, Ohkura S, Okamura H, Ikai A, Ichikawa M, Osada T. Fetal development of vomeronasal system in the goat, Brain Res Dev Brain Res, Vol. 149, pp. 113-120, 2004.
• Ikai, A., Afrin, R., Hertadi, R., Sekiguchi, H., Kim, H., Uehara, H., Wang, T., Alam, M.T., Arakawa, H., Osada, T. Nano-mechanics of protein molecules and cellular structures: A review of recent work done in the laboratory of biodynamics, Recent Res. Devel. Applied Physics, Vol. 6, pp. 717-732, 2003.
• Afrin R., Arakawa, H., Osada, T., Ikai, A. Extraction of Membrane Proteins from Living Cell Surface Using the Atomic Force Microscope and Covalent Crosslinkers, Cell Biophysics and Biochemistry, Vol. 39, pp. 101-118, 2003.
• 11. Muramoto, K., Osada, T., Kato-Negishi, M., Kuroda, Y., Ichikawa, M. Increase in the Number of Tyrosine Hydroxylase-Containing Neurons in the Primary Culture System of Rat Accessory Olfactory Bulb Induced by Coculture with Vomeronasal Pockets, Neuroscience, Vol. 116, No. 985-994, 2003.
• 10. Kim, H., Arakawa, H., Osada, T., Ikai, A. Quantification of Cell Adhesion Force with AFM, Ultramicroscopy, Vol. 97, pp. 359-363, 2003.
• Osada, T., Itoh, A., Ikai, A. Mapping of the receptor-associated protein (RAP) binding protein on living fibroblast cells using an atomic force microscope, Ultramicroscopy, Vol. 97, pp. 353-357, 2003.
• Osada, T., Uehara, H., Kim, H., Ikai A. mRNA analysis of single living cells, Journal of Nanobiotechnology, Vol. 1, pp. 2, 2003.
• Idiris A, Ohtsubo K, Yoza K, Osada T, Nakamichi N, Matsumura T, Ikai A. Molecular cloning and structural characterization of the hagfish proteinase inhibitor of the alpha-2-macroglobulin family, Journal of Protein Chemistry, Vol. 22, pp. 89-98, 2003.
• 12. Matsuoka, M, Osada, T, Yoshida-Matsuoka J., Ikai, A., Ichikawa, M., Norita, M., Costanzo, R. A comparative immunocytochemical study of development and regeneration of　chemosensory neurons in the rat vomeronasal system, Brain Research, Vol. 946, pp. 52-63, 2002.
• 15. Ikai, A., Afrin, R., Itoh, A., Thogersen, H. C., Hayashi, Y., Osada, T. Force Measurement for Membrane Protein Manipulation, Colloids and Surfaces B: Biointerfaces, Vol. 23, pp. 33-43, 2002.
• Yingchun Sun, Hideo Arakawa, Toshiya Osada, Atsushi Ikai. Tapping and Contact Mode Imaging of Native Chromosomes and Extraction of Genomic DNA using AFM Tips, Applied Surface Science, Vol. 188, pp. 499-505, 2002.
• Kim Hyonchol, Hideo Arakawa, Toshiya Osada, Atsushi Ikai. Quantification of Cell Adhesion Interactions by AFM: Effects of LPS/PMA on the adhesion of C6 glioma cell to collagen type I, Applied Surface Science, Vol. 188, pp. 493-498, 2002.
• 14. Kim, H., Arakawa, H., Osada, T., Ikai, A. Quantification of Fibronectin and Cell Surface Interactions by AFM, Colloids and Surfaces B: Biointerfaces, Vol. 25, pp. 33-43, 2002.
• Kim Hyonchol, Hideo Arakawa, Toshiya Osada, Atsushi Ikai . Quantification of Fibronectin and Cell Surface Interactions by AFM, Colloids and Surfaces B: Biointerfaces , 2001.
• Atsushi Ikai, Rehana Afrin, Arimich Itoh, Hans Christian Thogersen, Yoshiaki Hayashi, Toshiya Osada. Force Measurement for Membrane Protein Manipulation, Colloids and Surfaces B, 2001.
• J. Yoshida-Matsuoka, T. Osada, Y. Mori, M. Ichikawa . A developmental study using three antibodies (VOBM1, VOBM2, and VOM2): immunocytochemical and electron microscopical analysis of the luminal surface of the rat vomeronasal sensory epithelium., Anat. Embryol. , Vol. 199, pp. 215-224., 1999.
• 長田俊哉, 伊東有道, 荒川秀雄, 猪飼篤 . 原子間力顕微鏡によるフェロモン受容器の研究, Brain Medical , Vol. 11, pp. 54-59, 1999.
• Osada T, Ikai A, Costanzo RM, Matsuoka M, Ichikawa M. Continual neurogenesis of vomeronasal neurons in vitro, J Neurobiol, Vol. 40, pp. 226-233, 1999.
• Shu Takigami, Toshiya Osada, J. Yoshida-Matsuoka, Masato Matsuoka, Yuji Mori, Masumi Ichikawa . The expressed localization of rat putative pheromone receptors., Neurosci. Lett., Vol. 272, pp. 115-118., 1999.
• Osada, T., Takezawa, S., Itoh, A., Arakawa, H. Ichikawa, M., Ikai, A.. The distribution of sugar chains on the vomeronasal epithelium observed with the atomic force microscope, Chmical Senses, Vol. 24, pp. 1-6, 1999.
• TOSHIYA OSADA. Replacement of receptor cells in the hamster vomeronasal epithelium after nerve transection, Chemical Senses, Vol. 23, No. 2, pp. 171-179, 1998.
• TOSHIYA OSADA. Atomic force microscopy of histological sections using a new electron beam etching method, J. Microsc., Vol. 189, pp. 43-49, 1998.
• TOSHIYA OSADA. Neurite-promoting effect of α┣D22┫D2-macroglobulin in rat cerebral cortex is mainly associated with α┣D22┫D2-macroglobulin receptor, Brain Research, Vol. 737, pp. 269-274, 1996.
• 長田俊哉. 鋤鼻嗅覚神経細胞の発生と再生(共著), 蛋白質核酸酵素, Vol. 41, pp. 2070-2076, 1996.
• TOSHIYA OSADA. Differential binding patterns of three antibodies in the rat vomeronasal organ and accessory 01 factory bulb, Cell and Tissue Research, Vol. 281, pp. 243-248, 1995.
• M. Ichikawa, T. Osada . Morphology of vomeronasal organ cultures from fetal rat., Anat. Embryol., Vol. 191, pp. 25-32, 1995.
• TOSHIYA OSADA. Coculture of the vomeronasal organ, Anat. Embryol., Vol. 192, pp. 415-424, 1995.
• T. Osada, M. Ichikawa, R. M. Costanzo. Is nestin a marker for chemosensory precursor cells? , Brain Reseach., Vol. 683, pp. 254-257, 1995.
• T. Osada, K. Kito, K. Ookata, P.P.C. Graziadei, A.Ilai, M Ichikawa . Monoclonal antibody (VOM2) specific for the luminal surface of the rat vomeronasal sensory epithelium., Neurosci. Lett., Vol. 170, pp. 47-50, 1994.
• M. Ichikawa, T. Osada, P.P.C. Graziadei . Vicia Villosa agglutinin inhibits the fasciculation of vomeronasal axons in fetal rat vomeronasal organ culture. , Brain Res., Vol. 668, pp. 252-255, 1994.
• M. Ichikawa, S. Takami, T. Osada, P.P.C. Graziadei. Differential developmental of binding sites of two lectins in the vomeronasal axons of the rat accessory olfactory bulb. , Develop. Brain Res., Vol. 78, pp. 1-9, 1993.
• A. Ikai, S. Mitsuda, T.Nakagawa, T. Osada. a 2-macroglobulin:the molecule and its biomedical　applications., New Functionality Materials, Vol. B, pp. 217-221, 1993.
• S. Mitsuda, T.Nakagawa, T. Osada, T. Shimamoto, H. Nakazato, A. Ikai . A receptor mediated delivery of an HIV-1 derived peptide vaccine., Biochem. Biophys. Res. Commun., Vol. 194, pp. 1155-1160, 1993.
• S. Mitsuda, T.Nakagawa, T. Osada, A. Ikai . A receptor mediated antigen delivery and incorporation system: Administrations of a2-macroglobulin-cytochrome c conjugate induced high concentrations of antibodies against cytochrome c in mice., Biochem. Biophys. Res. Commun., Vol. 191, pp. 1326-1331, 1993.
• TOSHIYA OSADA. The active site titration of proteinases by using α┣D22┫D2-macroglolulin and high-performance liquif chromato gcaphy, Analytical Biochemistry, 1992.
• TOSHIYA OSADA. Bandeiraea Simplicifolin lectin I and Vicia Villosa agjlntinin bind specifically to the vomeronasal abons in the accessory olfactory bulb of the rat, Neuroscience Research, pp. 13, 1992.
• TOSHIYA OSADA. Reaction of proteinares with α┣D22┫D2-macroglobulin from the American house-shoe crab, ┣DBLimulus(/)-┫DB., J. Biol. Chem., pp. 266, 1991.
• TOSHIYA OSADA. Purification and characterization of α-macroglobulin and ovomacroglobulin of green turtle, J. Biochem., pp. 103, 1988.
• TOSHIYA OSADA. Polymerization of turtle α-macroglobulin through sulfhydryls reveals the location of ex-thiolester bonds., J. Ultrastruc. Mol. Struc. Res., pp. 101, 1988.
• TOSHIYA OSADA. Conformational change of α-macroglobulin and ovomacroglobulin of green turtle., J. Biochem., pp. 103, 1988.
• TOSHIYA OSADA. Antibodies against vila proteins can be produced effectively in response to the increased uptake of α┣D22┫D2-macroglobulin : viral protein conjugate by macrophage., Biochem. Biophys. Res. Commun., pp. 150, 1988.
• TOSHIYA OSADA. Endocytotic internalization of α┣D22┫D2M : α-galactosidase conjugate in fibroblasts from Fabry's hemizygote., Biochem. Biophys. Res Commun., pp. 142, 1987.
• TOSHIYA OSADA. Struetural change of α┣D22┫D2- and ovomacroglobulins, J. Protein. Chemistry, pp. 6, 1987.
• TOSHIYA OSADA. T cell proliferation can be specifically augmented by macrophages fed with specific antigrn : α┣D22┫D2M conjngate., Biochem. Biophys. Res. Commun., pp. 146, 1987.
• T. Osada, Y. Kuroda, A. Ikai . The fate of internalized a2M : a-galactosidase conjugate in fibroblasts from Fabry's hemizygote., Biochem. Biophys. Res. Commun., Vol. 143, pp. 954-958, 1987.
• T. Osada, M. Nishigai, A.Ikai . Open quaternary structure of the hagfish proteinase inhibitor with similar properties to human alpha-2-macroglobulin., J. Ultrastruc. Mol. Struct. Res., Vol. 96, pp. 136-145, 1986.
• H. Arakawa, T.Osada, A.Ikai. Unusual properties of crocodilian ovomacroglobulin shown in its methylamine treatment and sulfhydryl titration. , Arch. Biochem. Biopys. , Vol. 244, pp. 447-453, 1986.
• M. Nishigai, T. Osada, A. Ikai. Structural changes in alpha-2- and ovomacroglobulin studied by gel chromatography and electron microscopy., Biochem. Biophys. Acta , Vol. 831, pp. 236-241, 1985.

国内会議発表 (査読なし・不明)

• 田中潤子, 安部聡, 長田俊哉, 上野隆史. GPCR 内包を目指したタンパク質ハイブリッド結晶の構築, 第 10 回 CSJ 化学フェスタ, Oct. 2020.

その他の論文・著書など

• Kim, Hyonchol, Uehara, Hironori, Afrin, Rehana, Sekiguchi, Hiroshi, Arakawa, Hideo, TOSHIYA OSADA, Ikai, Atsushi. Application of Atomic Force Microscopy to the Study of Expressed Molecules in or on a Single Living Cell, BIOSYSTEMS INVESTIGATED BY SCANNING PROBE MICROSCOPY, SPRINGER, pp. 555-581, 2010.
• Osada, T., Uehara, H., Kim, H., Ikai A. Clinical laboratory implication of single living cell mRNA analysis, Clinical Chemistry, Vol. 38, pp. 239-257, 2004.

学位論文

• Molecular transport system using α2M and its receptor,  本文,  Doctor of Science,  東京大学,  1988/--/--, 

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